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mTagRFP-T-H2B-6

Plasmid

#58019

Purpose

Localization: Nucleus/Histones, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

H2B (H2BC11 Human)

Tags

mTagRFP

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pdCas9-omega

Plasmid

#114632

Purpose

Addgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAP

Depositor

Anushree Chatterjee

Article

Otoupal et al Commun Biol. 2018 Sep 3;1:129. doi: 10.1

Insert

dCas9-omega

Use

CRISPR

Expression

Bacterial

Promoter

Tet promoter

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pGK:HygroR-CMV:mKate2-B_UTR

Plasmid

#68480

Purpose

Lentivirus expressing mKate2 with UTR sensor cassette and hygromycin resistance

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Hygromycin Resistance
mKate2-B_UTR

Use

Lentiviral

Expression

Mammalian

Mutation

shRNA sensor cassette "B" added to 3&#x…

Promoter

CMV and pGK

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pKanCMV-mRuby3-18aa-Tubulin

Plasmid

#74256

Purpose

Mammalian expression of N-terminal fusion of mRuby3 to Tubulin

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

Tubulin

Tags

mRuby3

Expression

Mammalian

Promoter

CAG and CMV

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ10333

Plasmid

#183955

Purpose

CAG-dCas12a(WT)-HA-miniVPR-mCherry-T2A-HygroR

Depositor

Stanley Qi

Article

Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

Insert

Nuclease-dead Lb Cas12a

Use

CRISPR

Tags

HA, Hygromycin Resistance, and mCherry

Expression

Mammalian

Mutation

D832A

Promoter

CAG

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SUMO-A'

Plasmid

#209115

Purpose

Bacterial expression of CC-GEMS ligand SUMO-A'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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human EB1 in PET28a for protein expression

Plasmid

#39297

Depositor

Tim Mitchison, Jennifer Tirnauer

Article

Tirnauer et al Mol Biol Cell. 2002 Oct . 13(10):3614-26

Insert

EB1 (MAPRE1 Human)

Tags

6HIS

Expression

Bacterial

Promoter

T7

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pHR V5-RNF25 dRING_IRES-mCherry

Plasmid

#198387

Purpose

RNF25 (?RING) expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

RNF25 (RNF25 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

RING deletion (135-201)

Promoter

EF1A

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-LIN-41

Plasmid

#52716

Purpose

retroviral expression of human LIN-41 / TRIM71

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc CDC4 R465C

Plasmid

#16653

Depositor

Bert Vogelstein

Article

Rajagopalan et al Nature. 2004 Mar 4. 428(6978):77-81.

Insert

CDC4 R465C (FBXW7 Human)

Tags

myc

Expression

Mammalian

Mutation

R465C

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
PLK3 1-334-pEGFP

Plasmid

#23266

Depositor

Wei Dai

Article

Yang et al Cancer Res. 2008 Jun 1. 68(11):4077-85.

Insert

PLK3 (PLK3 Human)

Tags

pEGFP

Expression

Mammalian

Mutation

aa 1-334

Available Since

Dec. 20, 2012

Availability

Academic Institutions and Nonprofits only

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mCherry-C1-GOLGA4-GRIP

Plasmid

#220068

Purpose

overexpreeses the TGN protein GRIP with a mCherry fluorophore

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 22;7:2

Insert

GOLGA4-GRIP (GOLGA4 Human)

Expression

Mammalian

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMC-6-GFP-P2A-NptII-9

Plasmid

#197741

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

EGFP-P2A-NptII bicistronic CDS

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5 mSynCAM 2

Plasmid

#115497

Purpose

expression of mouse full-length SynCAM 2

Depositor

Thomas Biederer

Article

Fogel et al J Neurosci. 2007 Nov 14;27(46):12516-30.

Insert

Cadm2 (Cadm2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiX2-PURO-shLKB1-Ms

Plasmid

#61231

Purpose

Lentivirus expressing shRNA to mouse LKB1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

shLkb1 (Ms/Hu) (Stk11 Mouse, Human)

Use

Lentiviral

Promoter

H1/TO

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-CMV-CD4-sTC-M13-GFP-P2A-CaM-V5-sTN

Plasmid

#219781

Purpose

CaST-P2A for HEK cell expression

Depositor

Christina Kim

Article

Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

Insert

CD4-sTC-M13-GFP-P2A-CaM-V5-sTN

Use

AAV

Tags

HA and V5

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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mEos2-Mito-7

Plasmid

#57401

Purpose

Localization: Mitochondria, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Mito (COX8A Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pLKO mouse shRNA 1 raptor

Plasmid

#21339

Depositor

David Sabatini

Article

Thoreen et al J Biol Chem. 2009 Mar 20. 284(12):8023-3

Insert

raptor shRNA 1 (Rptor Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 7, 2009

Availability

Academic Institutions and Nonprofits only
ERroGFP_iE_C204Q_pCDNA3

Plasmid

#48629

Purpose

Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 204 to Glutamine mutation (roGFP C147, E147b, C204Q)

Depositor

David Ron

Article

Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

Insert

ERroGFP

Tags

FLAG

Expression

Mammalian

Mutation

insertion E147B, C204Q

Available Since

Oct. 8, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-CD4-FLAG

Plasmid

#51604

Purpose

expresses CD4 with C-terminal FLAG tag

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

CD4 (CD4 Human)

Tags

FLAG and myc

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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