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sg_Atm_i5-ipUSEPR-TR657

Plasmid

#228918

Purpose

Knockdown of Atm in mammalian cells

Depositor

Sika Zheng

Article

Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

Insert

sg_Atm_i5 (Atm Mouse)

Use

Lentiviral

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pFNC-4

Plasmid

#227668

Purpose

Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. SmR, easily curable via sucrose counterselection.

Depositor

Pablo Ivan Nikel

Article

Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

Insert

SmR

Expression

Bacterial

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-hfYFP-TEV-mScarlet-i

Plasmid

#191196

Purpose

Fusion of hfYFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

hfYFP & mScarlet-I fusion

Tags

6xHis and mScarlet-I

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-LSSmGFP-TEV-mScarlet-i

Plasmid

#191199

Purpose

Fusion of LSSmGFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP & mScarlet-I fusion

Tags

6xHis and mScarlet-I

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pB-3xFlag-dCas13-MBD6MBD

Plasmid

#228233

Purpose

For targeted tethering of the MBD domain of human MBD6 using dCas13

Depositor

Chuan He

Article

Zou et al Nature. 2024 Oct 2. doi: 10.1038/s41586-

Inserts

dCas13
MBD6 (MBD6 Human)

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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gRNA_SRR134.3_miRFP670

Plasmid

#163754

Purpose

gRNA expression vector containing the miRFP670 fluorescent marker to target the region downstream of the SRR134 SOX2 enhancer in human cells.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

gRNA_SRR134.3

Use

CRISPR

Tags

miRFP670

Expression

Mammalian

Promoter

U6

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pET3a-XlH3[ChEx]

Plasmid

#221200

Purpose

Xenopus laevis histone H3 with Nterminal tail swapped for ChEx domain of ScChd1.

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

Xl-H3[ChEx]

Expression

Bacterial

Available Since

July 11, 2024

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-hph-lacZ

Plasmid

#218370

Purpose

Empty backbone for lacZ fusions

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available Since

May 2, 2024

Availability

Academic Institutions and Nonprofits only

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pX458-tRNA-SpCas9

Plasmid

#195183

Purpose

Vector for transient Cas9 and EGFP expression. Small tRNA promoter for sgRNA cloning by GoldenGate.

Depositor

Stephanie Roessler

Insert

Cas9

Use

CRISPR

Promoter

tRNA-Gln

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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GAL4(1-147)

Plasmid

#195029

Purpose

The control vector GAL4 (1-147) was generated by introducing a stop codon immediately after the GAL4 (1-147) sequence in the GAL4-Creb5 (1-128) vector

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

GAL4 (1-147)

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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IAA17-4flag-HIS5

Plasmid

#207015

Purpose

For ORF tagging with IAA17-4Flag. IAA17 is an auxin-inducible degron (AID).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-OptoSTIM1(CRY2clust)

Plasmid

#184715

Purpose

OptoSTIM1(CRY2clust)

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

OptoSTIM1

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAXY0011

Plasmid

#205580

Purpose

NpuDnaE(C)_KAN(193-265)_RUBY

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

NpuDnaE(C)

Expression

Plant

Promoter

35S

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAXY0010

Plasmid

#205579

Purpose

KAN(1-192)-NpuDnaE(N)_GFPuv

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

NpuDnaE(N)

Expression

Plant

Promoter

35S

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAXY0009

Plasmid

#205578

Purpose

NpuDnaE(C)_KAN(132-265)_RUBY

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

NpuDnaE(C)

Expression

Plant

Promoter

35S

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAXY0008

Plasmid

#205577

Purpose

KAN(1-131)-NpuDnaE(N)_GFPuv

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

NpuDnaE(N)

Expression

Plant

Promoter

35S

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pTrc-CysGA-Linker only

Plasmid

#201080

Purpose

GS linker inserted into CysGA at G364

Depositor

Shu Quan

Article

Ren et al Proc Natl Acad Sci U S A. 2021 Mar 30;11

Insert

CysGA-Linker only

Expression

Bacterial

Promoter

pTrc

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAXY0018

Plasmid

#205587

Purpose

p3flag_HygR(1-52)-NpuDnaE(N)

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

HygR-NpuDnaE(N)_homo-codon-OP

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pGWB511-TP-UnaG-FLAG

Plasmid

#203479

Purpose

For Agrobacterium transformation. A plastid transit peptide fused UnaG-FLAG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Depositor

Yutaka Kodama

Article

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

Plastid transit peptide fused UnaG-FLAG

Expression

Bacterial and Plant

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pH6-2xmNeon

Plasmid

#178462

Purpose

A tandem mNeonGreen fluorescent protein (2xmNeon) with an N-terminal 6xHis-tag in pET28a.

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

mNeonGreen dimer

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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