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pFastBac1-ScVip1-536-1107-delta848-918-R547A-H548A-R551A

Plasmid

#238314

Purpose

ScVip1-536-1107-delta848-918-R547A-H548A-R551A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-delta848-918-R547A-H548A-R551A

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-E576A-K623A-Q625S-K627A

Plasmid

#238327

Purpose

ScVip1-536-1107-E576A-K623A-Q625S-K627A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-E576A-K623A-Q625S-K627A

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hRNF31_2b)-PGKpuro2ABFP-W

Plasmid

#208415

Purpose

Lentiviral gRNA plasmid targeting human RNF31 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

RNF31 (RNF31 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hRNF31_1k)-PGKpuro2ABFP-W

Plasmid

#208414

Purpose

Lentiviral gRNA plasmid targeting human RNF31 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

RNF31 (RNF31 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-K558A-K605A-K732A-K817A

Plasmid

#238325

Purpose

ScVip1-536-1107-K558A-K605A-K732A-K817A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-K558A-K605A-K732A-K817A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-GFP-pA U6 shRNA (Rat NPAS4 #1)

Plasmid

#233270

Purpose

To express GFP from a CMV promoter and to express and shRNA targeting Rat Npas4

Depositor

Jonathan Ploski, Glenn Schafe

Article

Ploski et al PLoS One. 2011;6(8):e23760. doi: 10.1371

Insert

GFP and shRNA targeting Rat NPAS4 (Npas4 Rat)

Use

AAV

Tags

GFP

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-GFP-pA U6 shRNA (Rat NPAS4 #5)

Plasmid

#233271

Purpose

To express GFP from a CMV promoter and to express and shRNA targeting Rat Npas4

Depositor

Jonathan Ploski, Glenn Schafe

Article

Ploski et al PLoS One. 2011;6(8):e23760. doi: 10.1371

Insert

GFP and shRNA targeting Rat NPAS4 (Npas4 Rat)

Use

AAV

Tags

GFP

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFPc1 QFR IP3R3

Plasmid

#236454

Purpose

transient overexpression of IP3R3 in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nature. 2017 Jun 22;546(7659):554-558. d

Insert

IP3R3 (Itpr3 Rat)

Expression

Mammalian

Mutation

Q-FR/A-AA

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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MZB035

Plasmid

#217834

Purpose

Bacterial expression of human AP3D1 (1-617) and AP3S1 AP-3 core hemicomplex

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Inserts

AP3D1 (AP3D1 Human)
AP3S1 (AP3S1 Human)

Tags

GST

Expression

Bacterial

Mutation

Removed residues 618-1153 from AP3D1

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_6xHis-TEV-mCherry-WIPI2d IDR (364-425aa)

Plasmid

#223790

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

WIPI2d IDR (WIPI2 Human)

Tags

6xHis-TEV-mCherry

Expression

Bacterial

Mutation

aa 364-425 only

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-mEGFP-WIPI2 IDR (364-425aa)

Plasmid

#223758

Purpose

Stable expression and inducible recruitment of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

WIPI2 IDR (WIPI2 Human)

Use

Lentiviral

Tags

FKBP-mEGFP

Mutation

aa 364-425 only

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_6xHis-TEV-mCherry-WIPI2d (K87A/K88A)

Plasmid

#223751

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

WIPI2d (WIPI2 Human)

Tags

6xHis-TEV-mCherry

Expression

Bacterial

Mutation

K87A/K88A

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1_GST-LC3C

Plasmid

#223727

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

LC3C (MAP1LC3C Human)

Tags

GST

Expression

Bacterial

Mutation

aa 2-126 only, with Glycine exposed

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG_GST-TEV-WIPI1

Plasmid

#223798

Purpose

Expression of recombinant protein for purification

Depositor

James Hurley, Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

WIPI1 (WIPI1 Human)

Tags

GST-TEV

Expression

Mammalian

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-EGFR

Plasmid

#222325

Purpose

Synchronize the trafficking of EGFR from the ER.

Depositor

Franck Perez

Article

Scharaw et al J Cell Biol. 2016 Nov 21;215(4):543-558.

Insert

Streptavidin-KDEL and EGFR fused to SBP-EGFP (EGFR Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pPICZ A-SLC-His+Strep-Opa1 (SB254)

Plasmid

#227603

Purpose

Inducible coexpression of His-tagged SLC25A46 and Strep-tagged Opa1 in Pichia pastoris

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Inserts

SLC25A46 (SLC25A46 Human)
Opa1

Tags

10xHis and Twin-StrepTag

Expression

Yeast

Mutation

contains aa 253-960 only

Promoter

AOX1

Available Since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pMX-Flag-GFP_TMEM88B

Plasmid

#227954

Purpose

Co-localisation experiment

Depositor

Alessandro Ori

Article

Ghoochani et al Cell. 2026 Feb 5;189(3):765-782.e31. doi

Insert

TMEM88B

Use

Lentiviral

Tags

3xFLAG-EGFP

Expression

Mammalian

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pPICZ A-SLC25A46[R247A,R347A]-His (SB257)

Plasmid

#227606

Purpose

Inducible expression of His-tagged SLC25A46 with R247A and R347A mutations in Pichia pastoris

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Tags

10xHis

Expression

Yeast

Mutation

R247A,R347A

Promoter

AOX1

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pPICZ A-Strep-Opa1(MGD) (SB252)

Plasmid

#227601

Purpose

Inducible expression of Strep-tagged Opa1(MGD) in Pichia pastoris

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

OPA1 (OPA1 Human)

Tags

Twin-StrepTag

Expression

Yeast

Mutation

contains aa 253-580 (MGD) and aa 938-960

Promoter

AOX1

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SLC25A46[R257A,R347A]-P2A-Electra1 (pAH36)

Plasmid

#227620

Purpose

Overexpression of SLC25A46 with R247A and R347A mutations in mammalian cell lines

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Tags

P2A-mElectra1

Expression

Mammalian

Mutation

R257A,R347A

Promoter

CMV, T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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