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pAc5.1/V5-HisA-RvCAHS3-GS-AcGFP1

Plasmid

#192469

Purpose

Transient expression of RvCAHS3 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cells

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS3

Use

Tags

AcGFP1

Expression

Insect

Mutation

Promoter

Ac5 promoter

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pEThT-RvCAHS8

Plasmid

#192467

Purpose

Bacterial expression of RvCAHS8

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS8

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pEThT-RvCAHS3

Plasmid

#192466

Purpose

Bacterial expression of RvCAHS3

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS3

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAcGFP1-N1-RvCAHS12-min-GS

Plasmid

#192465

Purpose

Expression of RvCAHS12-min-GS_linker-AcGFP1 in mammalian cells

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS12-min

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAcGFP1-N1-RvCAHS3-min-GS

Plasmid

#192463

Purpose

Expression of RvCAHS3-min-GS_linker-AcGFP1 in mammalian cells

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS3-min

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pM-mRuby2

Plasmid

#176550

Purpose

Expression of mRuby2 for auxotrophic selection in the absence of methionine

Depositor

Markus Ralser

Article

Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

Insert

yomRuby2

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH1(GAP)

Available since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pST_CAHS1_PARRC_150-189 (pBS0658)

Plasmid

#185201

Purpose

For the mammalian expression of the tardigrade protein CAHS1_PARRC_150-189. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS1_PARRC_150-189

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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PvLEA4_repeats_k3_26_mCh-SspB (pBS1075)

Plasmid

#185299

Purpose

Mammalian expression of sleeping chironomid protein PvLEA4_repeats_k3_26 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation.

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_shuffle_3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
DHN_VrDHN1a_mCh-SspB (pBS1074)

Plasmid

#185298

Purpose

Mammalian expression of riverbank grape plant protein DHN_VrDHN1a attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation.

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k3_26

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pvLEA22mer_shuffle_3_mCh-2xFKBP (pBS1119)

Plasmid

#185314

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_shuffle_3 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_shuffle_3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_APOE_HUMAN_A234G (pBS0816)

Plasmid

#185267

Purpose

For the mammalian expression of the human protein APOE_HUMAN_A234G. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_A234G

Use

Tags

Expression

Mammalian

Mutation

A234G

Promoter

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pST_Q19228_CAEEL_93-127 (pBS0735)

Plasmid

#185231

Purpose

For the mammalian expression of the C. elegans protein Q19228_CAEEL_93-127. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

Q19228_CAEEL_93-127

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_D4NWF2_DrHD_b03_dom3 (pBS0681)

Plasmid

#185207

Purpose

For the mammalian expression of the Deinococcus radiodurans protein D4NWF2_DrHD_b03_dom3. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

D4NWF2_DrHD_b03_dom3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_APOA4_ANDDA_21-367 (pBS0742)

Plasmid

#185234

Purpose

For the mammalian expression of the Chinese giant salamander protein APOA4_ANDDA_21-367. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOA4_ANDDA_21-367

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_Q7JLY3_CAEEL_48-124 (pBS0738)

Plasmid

#185232

Purpose

For the mammalian expression of the C. elegans protein Q7JLY3_CAEEL_48-124. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

Q7JLY3_CAEEL_48-124

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_Q19228_CAEEL_51-88 (pBS0734)

Plasmid

#185230

Purpose

For the mammalian expression of the C. elegans protein Q19228_CAEEL_51-88. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

Q19228_CAEEL_51-88

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_Q19228_CAEEL_51-127_noLink (pBS0733)

Plasmid

#185229

Purpose

For the mammalian expression of the C. elegans protein Q19228_CAEEL_51-127_noLink. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

Q19228_CAEEL_51-127_noLink

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_pvLEA22mer_shuffle_5 (pBS0731)

Plasmid

#185228

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_shuffle_5. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_shuffle_5

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_pvLEA22mer_shuffle_3 (pBS0729)

Plasmid

#185227

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_shuffle_3. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_shuffle_3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_pvLEA22mer_shuffle_1 (pBS0727)

Plasmid

#185226

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_shuffle_1. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_shuffle_1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge