Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 1,884 results for: nicola

Showing: 1121 - 1140 of 1884 results

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FUS LC-RGG1 QtoS

Plasmid

#226616

Purpose

All glutamines in the LC domain to serine

Depositor

Nicolas Fawzi

Publication

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC-RGG1 QtoS (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

All glutamine to serine in the LC domain

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC noYQ_noYT

Plasmid

#226617

Purpose

T7S / Q160S double mutation

Depositor

Nicolas Fawzi

Publication

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC noYQ_noYT (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

T7S / Q160S double mutation in LC domain to remov…

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC-RGG1 wild type

Plasmid

#226596

Purpose

Wild type sequence of FUS LC-RGG1 domains

Depositor

Nicolas Fawzi

Publication

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC-RGG1 WT (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

wild type LC-RGG1

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 12S->G

Plasmid

#226597

Purpose

12 serines to glycines

Depositor

Nicolas Fawzi

Publication

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 12S->A (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

12 serines to alanine

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 12S->A

Plasmid

#226598

Purpose

12 serines to alanines

Depositor

Nicolas Fawzi

Publication

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 12S->A (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

12 serines to alanine

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 12S->N

Plasmid

#226599

Purpose

12 serines to asparagines

Depositor

Nicolas Fawzi

Publication

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 12S->N (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

12 serines to asparagine

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
FUS LC 4QQ->AA

Plasmid

#226600

Purpose

4 glutamine pairs to alanine pairs at positions 27, 28, 92, 93, 132, 133, 157, 158

Depositor

Nicolas Fawzi

Publication

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 4QQ->AA (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

4 QQ pairs replaced with AA pairs at positions 27…

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 4QQ->GG

Plasmid

#226601

Purpose

4 glutamine pairs to glycine pairs at positions 27, 28, 92, 93, 132, 133, 157, 158

Depositor

Nicolas Fawzi

Publication

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 4QQ->GG (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

4 QQ pairs replaced with GG pairs at positions 27…

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 4QQ->NN

Plasmid

#226602

Purpose

4 glutamine pairs to asparagine pairs at positions 27, 28, 92, 93, 132, 133, 157, 158

Depositor

Nicolas Fawzi

Publication

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 4QQ->NN (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

4 glutamine pairs to asparagine pairs at position…

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
FUS LC 4S->A#1

Plasmid

#226603

Purpose

4 serines to alanine at residue positions 30, 44, 48, 53

Depositor

Nicolas Fawzi

Publication

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC S->A#1 (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

4 serines to alanine in sequence position subset …

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
FUS LC 4S->A#2

Plasmid

#226604

Purpose

4 serines to alanine at residue positions 70, 84, 89, 95

Depositor

Nicolas Fawzi

Publication

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 4S->A#2 (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

4 serines to alanine in sequence position subset …

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
FUS LC 4S->A#3

Plasmid

#226605

Purpose

4 serines to alanine at residue positions 108, 117, 119, 127

Depositor

Nicolas Fawzi

Publication

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 4S->A#3 (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

4 serines to alanine in sequence position subset …

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 8Y->S

Plasmid

#226606

Purpose

8 tyrosines to serine

Depositor

Nicolas Fawzi

Publication

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 8Y->S (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

8 tyrosines to serine

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
FUS LC StoG

Plasmid

#226607

Purpose

All serines to glycines

Depositor

Nicolas Fawzi

Publication

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC TtoS (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

All threonine to serine

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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hnRNPAB X2-FL

Plasmid

#216515

Purpose

Expresses MBP-tagged full length hnRNPAB X2

Depositor

Nicolas Fawzi

Publication

O'Connell et al Sci Rep. 2024 Nov 13;14(1):27890. doi: 1

Insert

hnRNPAB X2 FL

Tags

6xHis tags-MBP

Expression

Bacterial

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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hnRNPAB X2-RBD

Plasmid

#216516

Purpose

Expresses MBP-tagged RNA binding domain of hnRNPAB X2

Depositor

Nicolas Fawzi

Publication

O'Connell et al Sci Rep. 2024 Nov 13;14(1):27890. doi: 1

Insert

hnRNPAB X2-RBD

Tags

6xHis tags-MBP

Expression

Bacterial

Mutation

deleted amino acids 2-64 and 226-304

Available since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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hnRNPAB X2-IDR

Plasmid

#216517

Purpose

Expresses MBP-tagged intrinsically disordered region of hnRNPAB X2

Depositor

Nicolas Fawzi

Publication

O'Connell et al Sci Rep. 2024 Nov 13;14(1):27890. doi: 1

Insert

hnRNPAB X2-IDR

Tags

6xHis tags-MBP

Expression

Bacterial

Mutation

deleted amino acids 2-225

Available since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-Y115S/Y132S/Y139S

Plasmid

#216408

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y115/Y132/Y139 to serine mutations

Depositor

Nicolas Fawzi

Publication

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD-Y115S/Y132S/Y139S

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Tyrosine 115, 132 and 139 to Serine, and …

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-A51P/A56P

Plasmid

#216400

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56 to proline mutations

Depositor

Nicolas Fawzi

Publication

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD-A51P/A56P

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Alanine 51 and 56 to Proline, and deleted…

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-Q443P

Plasmid

#216401

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Q443 to proline mutation

Depositor

Nicolas Fawzi

Publication

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD-Q443P

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Glutamine 443 to Proline, and deleted ami…

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 1121 - 1140 of 1884 results

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