We narrowed to 56,480 results for: plasmid
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Plasmid#169605PurposeTier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (PmPGK1-Fluc-pA::A2-pA::PCREm4-Nluc-pA).DepositorInsertPPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
ExpressionMammalianPromoterPCREm4 / PPGKAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4_HLA-myc-GRM3 C127A-VC
Plasmid#140450PurposeMammalian expression plasmid for c-myc-tagged mGluR3 fused to the C-terminal half of split fluorescent Venus (VC)DepositorInsertGRM3 (GRM3 Human)
TagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationCysteine 127 to alanineAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH614
Plasmid#169603PurposeTier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (PmPGK1-Fluc-pA::PCREm4-Nluc-pA::A3-pA).DepositorInsertPPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
ExpressionMammalianPromoterPCREm4 / PPGKAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNIR-Fb21
Plasmid#184679PurposeNIR-Fb to spike-Cov-19 RBDDepositorAvailable SinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT5T_Rev7_1-211_SA112_KVK_Champ1_328-355
Plasmid#105638PurposeExpresses mouse monomeric Rev7 and fragment of Champ1 protein (amino acids 328-355) from bicistronic plasmidDepositorExpressionBacterialMutationMutations: F11S, G12A, V132K, C133V, A135KPromoterT7Available SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRBad-Z-C-wtBlc
Plasmid#168256PurposeC-fragment. This plasmid can be co-transformed with either Plasmid 168258: pET11a-Z-N-wtBlc or Plasmid 168473: pET11a-Z-N-DiB2 to obtain wtBlc‐split-Zip or DiB2‐split-Zip protein, correspondinglyDepositorInsertLeucine Zipper + C-fragment of wtBlc
ExpressionBacterialPromoteraraBADAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF9c/14
Plasmid#162820PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF9c/14DepositorInsertUb-(N-TAG)CoV2_ORF9c/14
UsePiggybacExpressionMammalianMutationCoV2_ORF9c/14(Met1,Amber)PromoterEF1alphaAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b(CoV1h)
Plasmid#162822PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b(CoV1h)DepositorInsertUb-(N-TAG)CoV2_ORF3b(CoV1h)
UsePiggybacExpressionMammalianMutationCoV2_ORF3b(CoV1h)(Met1,Amber)PromoterEF1alphaAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
GTU-c
Plasmid#81093PurposeGene Tagging vector URA/CFP - Plasmid for yeast enhanced monomeric CFP (yemCFP) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)DepositorInsertyemCFP
UseCre/LoxTagsyemCFPExpressionYeastAvailable SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
GTU-y
Plasmid#81095PurposeGene Tagging vector URA/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)DepositorInsertmCitrine
UseCre/LoxTagsmCitrineExpressionYeastMutationMutated mCitrine for monomeric isoform (A206K L22…Available SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
MIP 247 CoMiP 4in1 with shRNA p53
Plasmid#63726PurposeTo express Oct4, Klf4, Sox2, c-Myc and hairpin RNA p53. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).TagsIRES-dtomatoExpressionMammalianMutationcodon-optimisedPromoterSFFV for Pct4, Klf4, Sox2 and c-myc; U6 for p53Available SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-CIRBP
Plasmid#11607DepositorInsertCold Inducible RNA Binding Protein (CIRBP Human)
TagsHAExpressionMammalianMutationno mutationsAvailable SinceJuly 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pETM-30-GST-Dmp68_B
Plasmid#145962PurposeBacterial Expression of Dmp68DepositorInsertDmp68 (Rm62 Fly)
ExpressionBacterialAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-HA-rM3D(Gs)-IRES-mCitrine
Plasmid#50468PurposeGs-coupled rM3D-IRES-mCitrine under the control of CaMKIIa promoterDepositorInsertrM3D-IRES-mCitrine
UseAAVTagsHAMutationSee supplemental documents for DREADD mutationsPromoterCaMKIIaAvailable SinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-sCAG-mBaoJin-oPRE
Plasmid#218684PurposeAAV vector plasmid expressing mBaoJin under a short variant of the CAG (sCAG) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.DepositorInsertmBaoJin
UseAAVExpressionMammalianPromoterShort variant of the CAG promoterAvailable SinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-F-bpA-3xSV40pA-F-td-sfGFP
Plasmid#133573PurposeCAG tdGFP FLP/FRT reporter piggyBac transposon (bpA 3x SV40pA stop)DepositorInsertTandem dimer superfolder GFP
UseFlp/frtExpressionMammalianAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCW-Tet-Cas9-BFP-PGK-Blast
Plasmid#196720PurposeTet-inducible expression of Cas9-T2A-TagBFPDepositorInsertCas9-T2A-TagBFP
UseCRISPR and LentiviralTags3xFLAG-SV40NLS and Nucleoplasmin NLSPromoterTRE, PGKAvailable SinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG2-EGFP-C-IFT144
Plasmid#218740PurposeExpresses C-terminally EGFP-tagged IFT144 in mammalian cellsDepositorAvailable SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-hM3D(Gq)-mCherry
Plasmid#50478PurposeGq-coupled hM3D DREADD fused with mCherry under the control of GFAP promoterDepositorHas ServiceAAV5InserthM3D(Gq)-mCherry
UseAAVTagsmCherryMutationSee supplemental documents for DREADD mutationsPromoterGFAPAvailable SinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-SAGFP
Plasmid#127100PurposeSA-GFP substrate (Addgene #41594) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. SA-GFP cassette contains PGK-puromycin.DepositorInsertSA-GFP reporter
ExpressionMammalianAvailable SinceJune 24, 2019AvailabilityAcademic Institutions and Nonprofits only