We narrowed to 23,602 results for: crispr

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Tad-CBE(ACC2)

Plasmid

#235061

Purpose

Expresses Tad-CBE(ACC2) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(ACC2)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCXLE-dCas9VP192-T2A-EGFP-shP53

Plasmid

#69535

Purpose

Episomal plasmid encoding dCas9VP192 and p53 shRNA

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Insert

dCas9-dCas9VP192-GFP-shp53

Use

CRISPR

Tags

EGFP

Promoter

CAG

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pSLQ2806-2 pHR: U6-Sasgv2TRE3G CMV-mCherry

Plasmid

#84250

Purpose

Expresses optimized Sa sgTRE3G gRNA with a mCherry fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sa sgTRE3G

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pgRNAtet-lvaA

Plasmid

#106397

Purpose

Guide RNA plasmid targeting lvaA on BBR1-UP origin

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

sgRNA towards lvaA in Pseudomonas putida

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pRDB_270

Plasmid

#231111

Purpose

C>T base editor

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Insert

TadA-CDd-nCas9-SpG-UGI-HA

Use

CRISPR and Lentiviral

Mutation

D10A

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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gRNA_GFP-T1

Plasmid

#41819

Purpose

Expresses a guide RNA (gRNA) to target GFP (T1 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_GFP-T1

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pCyCBE

Plasmid

#226505

Purpose

A cytidine deaminase-mediated base-editing plasmid in cyanobacteria

Depositor

Weizhong Chen

Article

Li et al Commun Biol. 2024 Aug 14;7(1):994. doi:

Inserts

APOBEC1-Cas9D10A
sgRNA
sacB gene

Use

CRISPR

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pBA620

Plasmid

#186240

Purpose

LbuCas13a Negative Control (RFP targeting)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

pTet-LbuCas13a + crRNA negative control

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBA630

Plasmid

#186241

Purpose

RfxCas13d Negative Control (RFP targeting)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

pTet-RfxCas13d + crRNA negative control

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(CCG3)

Plasmid

#235074

Purpose

Expresses Tad-CBE(CCG3) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(CCG3)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-dCas9-VPR-pgk-hph

Plasmid

#233066

Purpose

Piggybac CRISPRa construct

Depositor

Markus Landthaler

Article

Vučićević et al bioRxiv 2024.09.12.612198

Insert

dCas9-VPR

Use

CRISPR

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pGRACE

Plasmid

#230910

Purpose

Tracing of CRISPR activity in whole animals.

Depositor

Michael Boutros

Article

Port et al bioRxiv 2024.11.26.625385

Insert

GFP

Use

CRISPR

Expression

Insect

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(ACC3)

Plasmid

#235062

Purpose

Expresses Tad-CBE(ACC3) in mammalian cells.

Depositor

Weixin Tang

Article

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Insert

Tad-CBE(ACC3)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, SV40NLS and bpNLS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBA615

Plasmid

#186239

Purpose

eLbuCas13a Negative Control (RFP targeting)

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Insert

pTet-eLbuCas13a + crRNA negative control

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE TRE dCas9

Plasmid

#50915

Purpose

Tet-regulatable dCas9 lentiviral expression vector

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A, H840A

Promoter

TRE

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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AiO-Cas12a: All in one enAsCas12a expression and gRNA cloning backbone

Plasmid

#228364

Purpose

mU6-driven expression of enAsCas12a gRNAs with EF1a-enAsCas12a-2A-Puro

Depositor

Michael Boettcher

Article

El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral; Enascas12a nuclease and en…

Expression

Mammalian

Promoter

mU6

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-3xFLAG-NLS-SpCas9-NLS

Plasmid

#51307

Purpose

Cas9 expression plasmid

Depositor

Yonglong Chen

Article

Guo et al Development. 2014 Jan 8.

Insert

3xFLAG-NLS-SpCas9-NLS

Use

CRISPR; Xenopus tropicalis expression

Tags

3x Flag and NLS

Promoter

CMV

Available Since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pMLS714

Plasmid

#188886

Purpose

MiniMos Transposon vector carrying Pmex-5::2xNLS::Cas9 and floxed Cbr-Unc-119

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Pmex-5::Cas9

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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ALKBH5H204A-dCas9

Plasmid

#134784

Purpose

Expression fusion protein ALKBH5H204A-dCas9 in Mammalian cells

Depositor

Shu-Bing Qian

Article

Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

Insert

ALKBH5 and inactive dCas9

Expression

Mammalian

Mutation

H204A

Promoter

CMV

Available Since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pMJ826

Plasmid

#39316

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Histidine 840 to Alanine (H840A)

Promoter

T7

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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