We narrowed to 23,296 results for: crispr

1. hCas9_D10A

   Plasmid

   #41816

   Purpose

   Expresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   Cas9_D10A
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A nickase

   Promoter

   CMV

   Available Since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28-FnCas12a-TEV

   Plasmid

   #174658

   Purpose

   FnCas12a expression plasmid

   Depositor

   Huimin Zhao, Jin Wang

   Article

   Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

   Insert

   FnCas12a
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBA620

   Plasmid

   #186240

   Purpose

   LbuCas13a Negative Control (RFP targeting)

   Depositor

   Jennifer Doudna

   Article

   Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

   Insert

   pTet-LbuCas13a + crRNA negative control
   

   Expression

   Bacterial

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBA630

   Plasmid

   #186241

   Purpose

   RfxCas13d Negative Control (RFP targeting)

   Depositor

   Jennifer Doudna

   Article

   Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

   Insert

   pTet-RfxCas13d + crRNA negative control
   

   Expression

   Bacterial

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSL103-SpCas9-FLAG-3XNLS

   Plasmid

   #182032

   Purpose

   Expression of recombinant Streptococcus pyogenes Cas9 protein carrying C-terminal FLAG and 3XNLS for genome editing as Cas9 RNP

   Depositor

   Steven Lin

   Article

   Lin et al Bio Protoc. 2022 May 20;12(10):e4420. do

   Insert

   Streptococcus pyogenes cas9 (cas9 Synthetic)
   

   Use

   CRISPR

   Tags

   12X His tag, 3XNLS, FLAG, and MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-CAG-dCas9-VPR-pgk-hph

   Plasmid

   #233066

   Purpose

   Piggybac CRISPRa construct

   Depositor

   Markus Landthaler

   Article

   Vučićević et al bioRxiv 2024.09.12.612198

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Available Since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBA615

   Plasmid

   #186239

   Purpose

   eLbuCas13a Negative Control (RFP targeting)

   Depositor

   Jennifer Doudna

   Article

   Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

   Insert

   pTet-eLbuCas13a + crRNA negative control
   

   Expression

   Bacterial

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMJ806

   Plasmid

   #39312

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tad-CBE(CCG3)

   Plasmid

   #235074

   Purpose

   Expresses Tad-CBE(CCG3) in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE(CCG3)-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, bpNLS and bpNLS

   Expression

   Mammalian

   Available Since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmCherry-U6-RNF103-CHMP3

    Plasmid

    #140584

    Purpose

    Expresses a gRNA and mCherry in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    gRNA RNF103-CHMP3
    

    Use

    CRISPR

    Available Since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. gRNA_GFP-T1

    Plasmid

    #41819

    Purpose

    Expresses a guide RNA (gRNA) to target GFP (T1 target sequence) for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    gRNA_GFP-T1
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRDA_336

    Plasmid

    #179095

    Purpose

    BE Cas expression

    Depositor

    John Doench, David Root

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    BE3.9 (Cas9-NG)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2-3xFLAG-NLS-SpCas9-NLS

    Plasmid

    #51307

    Purpose

    Cas9 expression plasmid

    Depositor

    Yonglong Chen

    Article

    Guo et al Development. 2014 Jan 8.

    Insert

    3xFLAG-NLS-SpCas9-NLS
    

    Use

    CRISPR; Xenopus tropicalis expression

    Tags

    3x Flag and NLS

    Promoter

    CMV

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. AiO-Cas12a: All in one enAsCas12a expression and gRNA cloning backbone

    Plasmid

    #228364

    Purpose

    mU6-driven expression of enAsCas12a gRNAs with EF1a-enAsCas12a-2A-Puro

    Depositor

    Michael Boettcher

    Article

    El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral; Enascas12a nuclease and en…

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pScI_dCas9-CDA_J23119-sgRNA

    Plasmid

    #108550

    Purpose

    Bacterial Target-AID vector with sgRNA

    Depositor

    Akihiko Kondo

    Article

    Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

    Inserts

    dCas9-PmCDA1
    Streptococcus pyogenes sgRNA
    

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    D10A and H840A for SpCas9

    Promoter

    J23119 and lambda OR

    Available Since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUAS-PE2-attB

    Plasmid

    #149550

    Purpose

    Expression of PE2 enzyme under control of the Gal4-regulated UAS promoter. Can be used to generate transgenic flies with white+ selection.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    PE2
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    UAS

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Tad-CBE(GCC3)

    Plasmid

    #235078

    Purpose

    Expresses Tad-CBE(GCC3) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(GCC3)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, bpNLS and bpNLS

    Expression

    Mammalian

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. Tad-CBE(GCG4)

    Plasmid

    #235080

    Purpose

    Expresses Tad-CBE(GCG4) in mammalian cells.

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

    Insert

    Tad-CBE(GCG4)-SpCas9 D10A
    

    Use

    CRISPR

    Tags

    2xUGI, bpNLS and bpNLS

    Expression

    Mammalian

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAID6

    Plasmid

    #136989

    Purpose

    Yeast integration plasmid for expression of dLbCpf1-VP, dSpCas9-RD1152, SaCas9, and Csy4

    Depositor

    Huimin Zhao

    Article

    Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

    Inserts

    dLbCpf1-VP
    dSpCas9-RD1152
    SaCas9
    Csy4
    

    Expression

    Yeast

    Promoter

    ENO2, TDH3, TEF1, and TPI1

    Available Since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. 4xNLS-pMJ915v2

    Plasmid

    #88917

    Purpose

    For expression of modified SpyCas9 in e.coli.

    Depositor

    Jennifer Doudna

    Article

    Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Inserts

    Cas9
    T7
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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