We narrowed to 27,048 results for: STI;
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Plasmid#89325PurposeInducible expression of C448Y spastin M1 and M87 in mammalian cellsDepositorAvailable SinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only
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pTRE/SP M1C448Y
Plasmid#89326PurposeInducible expression of M1C448Y spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
ExpressionMammalianMutation1-194 bp of 5'UTR deleted, C448YPromoterTet-responsive P tightAvailable SinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999)
Plasmid#185914PurposepCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8e-nSpCas9-NG-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…PromoterCMV and T7Available SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLIB-HIS-TEV-scCAK1
Plasmid#177014PurposeGenerate baculovirus for insect cell expression of scCAK1 with N-terminal Polyhistidine-TEVDepositorInsertCAK1 cyclin-dependent protein kinase-activating kinase CAK1 (CAK1 Budding Yeast)
TagsPolyhistidine-TEVExpressionInsectAvailable SinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164)
Plasmid#185919PurposepCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8.20m-nSpCas9-NG-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…PromoterCMV and T7Available SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
hprtSAGFP
Plasmid#41594PurposeIn vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequencesDepositorInsertSA-GFP reporter
UseMouse Targeting; Dsb repair reporter substrateExpressionMammalianAvailable SinceDec. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
plenti6-SLC7A11/xCT-V5
Plasmid#170427PurposeEepresses SLC7A11 (also named xCT) in mammalian cellsDepositorInsertsolute carrier family 7 member 11 (SLC7A11 Human)
UseLentiviralTagsV5ExpressionMammalianAvailable SinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)
Plasmid#185913PurposepCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337RPromoterCMV and T7Available SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.0 FLAG TRPV4
Plasmid#45751DepositorAvailable SinceAug. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRosetta_v2
Plasmid#136477PurposeLentiviral GFP Reporter assay for determining appropriate dose of puromycin, blasticidin, and hygromycinDepositorInsertHygroR-T2A-BlastR-P2A-PuroR-F2A-EGFP
UseLentiviralPromoterPGKAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AC EJ7-GFP
Plasmid#113617PurposeReporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFPDepositorInsertEJ7-GFP variant of EGFP
ExpressionMammalianAvailable SinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AC 4-μHOM
Plasmid#113619PurposeReporter for Alt-EJ, variant of EJ7-GFP. Double strand breaks are induced with the 7a & 7h, or 7a & 7i sgRNAs, with CAS9. EJ using 4 nt of microhomology causes a deletion mutation and restores GFPDepositorInsert4-μHOM variant of EGFP
ExpressionMammalianAvailable SinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
iTol2Amp-γ-crystallin:RFP
Plasmid#108455PurposeThis plamid can be used to recombine the iTol2-Amp-γ-crystallin:RFP into a BAC.DepositorInsertTol2-Amp-y-cryst-mCherry
UseGatewayAvailable SinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
Piggybac-TetO-hNurr1-blast
Plasmid#176485PurposeTetO-hNurr1 in a PiggyBac vector with Blasticidin resistanceDepositorAvailable SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
I48 ELP
Plasmid#68937PurposeElastin-like polypeptide (VPGxG) with 48 repeats of Isoleucine guest residueDepositorInsertI48 ELP
ExpressionBacterialPromoterT7Available SinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8.20m-nSpCas9-VRQR-P2A-EGFP (KAC1166)
Plasmid#185918PurposepCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8.20m-nSpCas9-VRQR-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337RPromoterCMV and T7Available SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Barr2 flag
Plasmid#14685DepositorAvailable SinceJune 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLV_TRET_hNgn1_UBC_Bla
Plasmid#61473Purpose3rd generation lentiviral vector; TetON promoter driving human Ngn1; constitutive expression of Blasticidine selection markerDepositorInsertsUseLentiviralAvailable SinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b SARS-CoV PLpro
Plasmid#61693Purposeinducible expression of SARS PLpro proteaseDepositorInsertSARS-CoV PLpro
TagsHisExpressionBacterialPromoterT7Available SinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-Cys-ELP(40nm)-LPETGG
Plasmid#90475PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGGDepositorInsertCys-ELP(40nm)-LPETGG
TagsCysteine and Sortase-TagExpressionBacterialPromoterT7Available SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only