We narrowed to 4,045 results for: golden gate
-
Plasmid#239504PurposeExpress GAL-inducible cytosolic sfCherry3C large fragment and Pex22 peroxisomal chauffeur with medium affinity SH3 peptideDepositorInsertsfCherry3C(1-10)-dPTS1
ExpressionYeastAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1171
Plasmid#239505PurposeExpress GAL-inducible cytosolic sfCherry3C large fragment and Pex22 peroxisomal chauffeur with high affinity SH3 peptideDepositorInsertsfCherry3C(1-10)-dPTS1
ExpressionYeastAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1517
Plasmid#239518PurposeExpress ClPT1(37-407) membrane enzyme cargo and Pex22 peroxisomal chauffeurDepositorInsert2xCrk_SH3-mTurquoise2-ClPT1(37-407)
ExpressionYeastAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1520
Plasmid#239520PurposeExpress CpPT1(52-408) membrane enzyme cargo and inactive Pex22 peroxisomal chauffeurDepositorInsert2xCrk_SH3-mTurquoise2-CpPT1(52-408)
ExpressionYeastAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1518
Plasmid#239519PurposeExpress CpPT1(52-408) membrane enzyme cargo and Pex22 peroxisomal chauffeurDepositorInsert2xCrk_SH3-mTurquoise2-CpPT1(52-408)
ExpressionYeastAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1138
Plasmid#239499PurposeExpress GAL-inducible peroxisomal sfCherry3C large fragment and Pex22 peroxisomal chauffeur with medium affinity SH3 peptideDepositorInsertsfCherry3C(1-10)-ePTS1
ExpressionYeastAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1139
Plasmid#239500PurposeExpress GAL-inducible peroxisomal sfCherry3C large fragment and Pex22 peroxisomal chauffeur with high affinity SH3 peptideDepositorInsertsfCherry3C(1-10)-ePTS1
ExpressionYeastAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_2H7W
Plasmid#234220PurposeHeterologous protein expression of chagasin in Escherichia coliDepositorInsert2H7W (Tc00.1047053506801.80 Trypanosoma cruzi)
Tags10x HisTag-Smt3ExpressionBacterialPromoterT7Available SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_2NT3
Plasmid#234208PurposeHeterologous protein expression of response regulator homolog in Escherichia coliDepositorInsert2NT3
Tags10x HisTag-Smt3ExpressionBacterialPromoterT7Available SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
U22m
Plasmid#121025PurposeMoClo golden gate assembly BC part (high expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).DepositorInsertBCD6
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C32m
Plasmid#120967PurposeMoClo golden gate assembly CD part for CinR (Quorum-sensing receptor for 3OC14). Please see Supplemental Documents for annotated Genbank file.DepositorInsertohCD carb CinR
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C36m
Plasmid#120970PurposeMoClo golden gate assembly CD part for RhlI (quorum-sensing synthase for 3OC14). Please see Supplemental Documents for annotated Genbank file.DepositorInsertohCD carb RhlI
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C40m
Plasmid#120973PurposeMoClo golden gate assembly CD part for blank part (random 50bp sequence) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertBlank (Random 50bp sequence)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C59m
Plasmid#120976PurposeMoClo golden gate assembly CD part for mScarlet (RFP variant; see https://www.fpbase.org/protein/mscarlet/). Please see Supplemental Documents for annotated Genbank file.DepositorInsertmScarlet
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C13m
Plasmid#120960PurposeMoClo golden gate assembly CD part for Cerulean (modified CFP; variant of https://www.fpbase.org/protein/cerulean/) - Please see Supplemental Documents for annotated Genbank fileDepositorInsertCerulean
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P1m
Plasmid#120934PurposeMoClo golden gate assembly AB part plasmid for J23101 (strength 1791x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson); AB overhangs.DepositorInsertJ23101
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICSL11086_GG_Wheat_live_Cas9_rev
Plasmid#126882PurposeEncodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.DepositorInsertWheat_live_Cas9
UseCRISPRAvailable SinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV10.F6.loxP
Plasmid#63201PurposepCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceOct. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pECNUS4_MP
Plasmid#228358PurposeABE8e-SpCas9-NG Multiplex Acceptor clone for insertion of up to six gRNA cassettes via Golden Gate with BpiI and T4 ligase, blue-white screeningDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterRPS5AAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
(B) G418 marker (R) (SBE 137)
Plasmid#195011Purposegeneticin (G418) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tNOSDepositorInsertpXYL+G418+tNOS
ExpressionBacterialPromoterpXYLAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only