We narrowed to 5,501 results for: golden gate

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PB-hEF1A-MG-U6-sgRNA

Plasmid

#175505

Purpose

All-in-one piggyBac transposon destination vector for hEF1a expression of Mega Gate cloned elements and hU6 expression of Golden Gate cloned guide RNAs

Depositor

George Church

Publication

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

hEF1a, U6

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBP_sgRNA

Plasmid

#190128

Purpose

Level 0 MoClo plasmid for Golden Gate cloning of sgRNAs

Depositor

Veronica Godoy-Carter

Publication

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Type

Empty backbone

Use

CRISPR

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pICSL4723-P1

Plasmid

#191786

Purpose

Level P Golden gate accepter

Depositor

Wendy Harwood

Publication

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Type

Empty backbone

Expression

Plant

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCR8/GW:U6

Plasmid

#85981

Purpose

Golden Gate Level 0 cassette encoding the U6 promoter

Depositor

Thomas Mock

Publication

Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

Insert

T. pseudonana U6 promoter

Use

CRISPR; Diatom (t. pseudonana)

Promoter

none

Available since

Jan. 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBR_attB(bxb)_ccdB_lox_v2

Plasmid

#198040

Purpose

Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

Depositor

Richard Davis

Publication

Blanch-Asensio et al Nat Protoc. 2024 Aug 23. doi: 10.1038/s4

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAGM4723:TpCC_Urease

Plasmid

#85982

Purpose

Golden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAs

Depositor

Thomas Mock

Publication

Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

Inserts

Cas9
Urease-targeting gRNA 1
Urease-targeting gRNA 2

Use

CRISPR; Diatom (t. pseudonana)

Tags

NLS and YFP

Promoter

FCP and U6

Available since

Jan. 18, 2017

Availability

Academic Institutions and Nonprofits only

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EC10161

Plasmid

#154060

Purpose

Level 0.5 Golden Gate backbone, containing loxP sites.

Depositor

Cristobal Uauy

Publication

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

N/A

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pICSL90016

Plasmid

#117514

Purpose

Cas9_2 Golden Gate Level 0

Depositor

Jonathan D Jones

Publication

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:AATG:Cas9_2:GCTT:BsaI

Use

CRISPR

Tags

NLS_SV40

Expression

Plant

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ135A

Plasmid

#69277

Purpose

Golden Gate entry vector; 5th gRNA under AtU6 promoter

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pMOD_C'0000a

Plasmid

#127226

Purpose

Empty Backbone for golden gate cloning compatible with Cermak., et al Toolkit.

Depositor

Daniel Voytas

Publication

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Type

Empty backbone

Expression

Plant

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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EKv413(Gold Cas-guide insert4 2 gene version)

Plasmid

#248342

Purpose

gRNA insert vector for Golden Gate Assembly, 2 gene or 4 gRNAs version

Depositor

Elena Kozgunova

Publication

Kozgunova et al bioRxiv 2025.07.29.667574

Type

Empty backbone

Use

CRISPR

Available since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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EKv414(Gold Cas-guide insert6 3 gene version)

Plasmid

#248343

Purpose

gRNA insert vector for Golden Gate Assembly, 3 gene or 6 gRNAs version

Depositor

Elena Kozgunova

Publication

Kozgunova et al bioRxiv 2025.07.29.667574

Type

Empty backbone

Use

CRISPR

Available since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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EKv376(gRNAscaffold-tRNA for cloning)

Plasmid

#248333

Purpose

Plasmid contains gRNA scaffold-tRNA and is used to amplify fragments for Golden Gate assembly

Depositor

Elena Kozgunova

Publication

Kozgunova et al bioRxiv 2025.07.29.667574

Insert

gRNAscaffold-tRNA

Use

CRISPR

Available since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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sgRNA_expression_vector

Plasmid

#210212

Purpose

An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.

Depositor

Albert Jeltsch

Publication

Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi

Type

Empty backbone

Expression

Bacterial and Mammalian

Promoter

U6

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pYPQ136A

Plasmid

#69278

Purpose

Golden Gate entry vector; 6th gRNA under AtU6 promoter

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ145

Plasmid

#69297

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 5 gRNAs

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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IBMc460

Plasmid

#161940

Purpose

Carries Golden Gate substitution insert for IBM with gp41-1 intein. PPhlF as C-lobe promoter

Depositor

Baojun Wang

Publication

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB1A3-BbsI-GY-gp-41-1N-cat-PPhlF-RiboJ-B32-gp41-1C-SS-SapI

Expression

Bacterial

Promoter

PPhlF

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ2369 - intron GG1 - 250bp

Plasmid

#159877

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

intron GG1 - 250bp

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMOD_D0000a

Plasmid

#127230

Purpose

Empty Backbone for golden gate cloning compatible with Cermak., et al Toolkit.

Depositor

Daniel Voytas

Publication

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Type

Empty backbone

Expression

Plant

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pICH47742:FCP:Cas9YFP

Plasmid

#85986

Purpose

Golden Gate Level 1 cassette encoding a Cas9-YFP fusion under the FCP promoter

Depositor

Thomas Mock

Publication

Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9

Use

CRISPR; Golden gate assembly

Tags

SV40 NLS, YFP

Promoter

FCP

Available since

Jan. 18, 2017

Availability

Academic Institutions and Nonprofits only

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