We narrowed to 15,769 results for: LEA

1. pGBW-m4046883

   Plasmid

   #145693

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp3

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;FLAG_2

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pGBW-m4046735

   Plasmid

   #149234

   Purpose

   Bacterial expression plasmid for SARS-CoV-2 ORF8 protein

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 ORF8 protein (ORF8 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;HA

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   May 11, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. EC0_2_VP64

   Plasmid

   #163714

   Purpose

   Plasmid encoding VP64 - SV40 NLS as a Type 4a part to be used in the Dueber YTK system (MoClo kit)

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Insert

   VP64 + SV40 NLS
   

   Use

   Synthetic Biology; Moclo vector level 0 (type 4a)

   Available Since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-NLS-ZapCmR1.1

   Plasmid

   #59011

   Purpose

   Nuclear zinc-binding FRET biosensor

   Depositor

   Amy Palmer

   Article

   Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

   Insert

   NLS-ZapCmR1.1 genetically encoded Zn(II) sensor
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   See comment

   Promoter

   CMV

   Available Since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pRS315e_pGal-dCas9-SH3

   Plasmid

   #79613

   Purpose

   Expresses dCas9-SH3 in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Tags

   SH3 domain

   Expression

   Yeast

   Mutation

   D10A and H840A for nuclease deficient Cas9

   Promoter

   pGal1

   Available Since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-MCS-YFP-NLS

   Plasmid

   #145274

   Purpose

   Mammalian Expression of Nuclear YFP with N-terminal MCS and SV40 NLS

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   MCS and YFP
   

   Expression

   Mammalian

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGBW-m4136460

   Plasmid

   #149532

   Purpose

   Bacterial expression plasmid for SARS-CoV-2 E (envelope)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   6xHIS;Linker_GGSG;MBP;Linker_GGSG;Cleavable TEV

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pGBW-m4046841

   Plasmid

   #145578

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 helicase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;FLAG_2

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. p3E-SGTAP no-pA

   Plasmid

   #82599

   Purpose

   3' entry vector containing streptavidin binding protein (SBP) followed by a TEV cleavage site and 2 protein G copies without pA for tandem affinity purification

   Depositor

   Kryn Stankunas

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   SBP-TEV-2x protein G
   

   Promoter

   None

   Available Since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. Split-RESCUE-C

    Plasmid

    #170154

    Purpose

    AAV vector carrying the RESCUE-DDC-λN (RESCUE is the evolved ADAR2 variant for C-U editing) with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    RESCUE-DDC-λN-NES
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21a - SodB

    Plasmid

    #89613

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase B protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Superoxide dismutase B
    

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Available Since

    April 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. ARB342

    Plasmid

    #124025

    Purpose

    Encodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear mAzamiGreen

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    LS-A4_EF1a-NLS::mAzamiGreen-bgPA_R1
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. ptCMV-153/47-VR-NG

    Plasmid

    #50704

    Purpose

    Final destination vector

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

    Insert

    TALEN-FokI nuclease backbone
    

    Use

    TALEN

    Tags

    Flag

    Promoter

    CMV

    Available Since

    May 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3 Glt1b

    Plasmid

    #98706

    Purpose

    mammalian expression of Glt1b

    Depositor

    Paul Rosenberg

    Article

    Chen et al J Neurosci. 2002 Mar 15;22(6):2142-52.

    Insert

    Glt1b (Slc1a2 Rat)
    

    Expression

    Mammalian

    Mutation

    I348V and N365Y (please see depositor comments se…

    Promoter

    CMV

    Available Since

    July 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA-FRT-FAK-kinase0

    Plasmid

    #59122

    Purpose

    FAK fret control sensor without FERM domain with no linker

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGK415-­phlTA2-­1E

    Plasmid

    #165970

    Purpose

    Expresses phlTA(2-­1E) mutant

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    phlF
    

    Use

    Synthetic Biology

    Tags

    Nuclear localization signal and three copies of V…

    Expression

    Yeast

    Mutation

    P5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…

    Promoter

    pPGK1 mutant

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. V32M,L84M CoV-2 ORF8

    Plasmid

    #159934

    Purpose

    Protein overexpression in E.coli

    Depositor

    James Hurley

    Article

    Flower et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

    Insert

    CoV-2 ORF8 18-121 (ORF8 CoV-2)
    

    Tags

    6x Histidine, TEV cleavable

    Expression

    Bacterial

    Mutation

    V32M, L84M

    Promoter

    T7

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. PHF13 (3O70)

    Plasmid

    #28154

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PHD finger protein 13 (PHF13 Human)
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    PHD-type zinc finger domain (residues 232-281).

    Available Since

    Feb. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pGBW-m4136462

    Plasmid

    #149533

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    6xHIS;Linker_GGSG;MBP;Linker_GGSG;Cleavable TEV

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pGBW-m4046932

    Plasmid

    #145750

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp9

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp9 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge