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We narrowed to 60,327 results for: cloning

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Showing: 11681 - 11700 of 60327 results

  1. pNYCOMPS-N-Lic-His-FLAG

    Plasmid
    #182506
    Purpose
    pET based vector for protein expression in E.coli for targets fused with N-terminal Flag and His
    Depositor
    Renato Bruni
    Type
    Empty backbone
    Tags
    10xHis-FLAG-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    182506-plasmid-map-sequence-id-358073

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  2. pLV Nup88-V5

    Plasmid
    #175117
    Purpose
    Lentiviral expression of mouse Nup88-V5
    Depositor
    Larry Gerace
    Insert
    Nup88 (Nup88 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175117-plasmid-map-sequence-id-351804

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  3. pLV Rbmxl1-V5

    Plasmid
    #175124
    Purpose
    Lentiviral expression of mouse Rbmxl1-V5
    Depositor
    Larry Gerace
    Insert
    Rbmxl1 (Rbmxl1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    175124-plasmid-map-sequence-id-351936

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  4. mU6-sgNeo1-hU6-sgNT

    Plasmid
    #177211
    Purpose
    Expresses a Neomycin-targeting gRNA (mU6), a non-targeting gRNA (hU6) and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgNeo/sgNT1
    Use
    Lentiviral
    Promoter
    mU6/hU6
    Available Since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177211-plasmid-map-sequence-id-349887

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  5. pKSB- attP 3xP3-GFPsv40 GGGG AAGA

    Plasmid
    #174534
    Purpose
    attP site and 3xP3-GFP fluorescence marker cassette for gene knock-in in mosquitoes
    Depositor
    Eric Marois
    Insert
    attP and 3xP3-GFP-SV40 BsaI cassette
    Expression
    Insect
    Promoter
    3xP3
    Available Since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    174534-plasmid-map-sequence-id-343228

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  6. pKSB- PUb-GFPsv40 GGGG AAGA

    Plasmid
    #174536
    Purpose
    PUb-GFP fluorescence marker cassette for gene knock-in in mosquitoes
    Depositor
    Eric Marois
    Insert
    PUb-GFP-SV40 BsaI cassette
    Expression
    Insect
    Promoter
    Aedes aegypti Polyubiquitin
    Available Since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    174536-plasmid-map-sequence-id-343240

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  7. pKSB- PUb-GFPTub56D GGGG AAGA

    Plasmid
    #174537
    Purpose
    PUb-GFP fluorescence marker cassette for gene knock-in in mosquitoes, with DmTub56D terminator
    Depositor
    Eric Marois
    Insert
    PUb-GFP-Tub56Dterminator BsaI cassette
    Expression
    Insect
    Promoter
    Aedes aegypti Polyubiquitin
    Available Since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    174537-plasmid-map-sequence-id-343241

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  8. pANY4-pL18-ccdB

    Plasmid
    #164898
    Purpose
    Two valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pL18-ccdB.
    Depositor
    Yingfeng An
    Insert
    ccdB cassette
    Tags
    His-tag
    Expression
    Bacterial
    Available Since
    April 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    164898-plasmid-map-sequence-id-327989

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  9. HCP2

    Plasmid
    #166104
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination
    Depositor
    Matthias Heinemann
    Insert
    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166104-plasmid-map-sequence-id-330375

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  10. HCP3

    Plasmid
    #166105
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination
    Depositor
    Matthias Heinemann
    Insert
    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166105-plasmid-map-sequence-id-330319

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  11. HCP7

    Plasmid
    #166109
    Purpose
    This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Whi5 and the other targets the C-terminus of Hta2.
    Depositor
    Matthias Heinemann
    Insert
    Whi5-sg106 (WHI5 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166109-plasmid-map-sequence-id-330317

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  12. pNSU299_APL3

    Plasmid
    #166073
    Purpose
    Plasmid for constituive spCas9 and tet-inducible APL1-targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    APL3 (APL3 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tet-inducible
    Available Since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166073-plasmid-map-sequence-id-329133

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  13. pNSU299_PPE1

    Plasmid
    #166083
    Purpose
    Plasmid for constitutive spCas9 and tet-inducible PPE1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    PPE1 (PPE1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tet-inducible
    Available Since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166083-plasmid-map-sequence-id-329122

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  14. thoc5.018.389

    Plasmid
    #137266
    Purpose
    Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.
    Depositor
    Tara Davis
    Insert
    thoc5 (THOC5 Human)
    Tags
    (His)6-TEV
    Expression
    Bacterial
    Mutation
    018-389
    Available Since
    Feb. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    137266-plasmid-map-sequence-id-323966

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  15. NKAP.001.140

    Plasmid
    #137206
    Purpose
    Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.
    Depositor
    Tara Davis
    Insert
    NKAP (NKAP Human)
    Tags
    (His)6-TEV
    Expression
    Bacterial
    Mutation
    001-140
    Available Since
    Jan. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    137206-plasmid-map-sequence-id-322594

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  16. Nup54-mEGFP494(2)

    Plasmid
    #163428
    Purpose
    Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of the mEGFP
    Depositor
    Sanford Simon
    Insert
    Nup54 (NUP54 Human)
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163428-plasmid-map-sequence-id-321665

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  17. pGEX4-4T-1 Pontibacillus AGSC

    Plasmid
    #161814
    Purpose
    Expresses AGSC domain from Pontibacillus A/G cyclase enzyme
    Depositor
    Douglas Feldman
    Insert
    AGSC domain
    Tags
    His and TEV
    Expression
    Bacterial
    Available Since
    Dec. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161814-plasmid-map-sequence-id-319428

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  18. pLIX403-EBFP2-ccdB-EYFP

    Plasmid
    #158558
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EBFP2
    Expression
    Mammalian
    Available Since
    Oct. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    158558-plasmid-map-sequence-id-309874

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  19. pXZ13lacZalphaKanR

    Plasmid
    #160230
    Purpose
    Like pXZ13lacZalpha, but adds second level of selection with Kanamycin resistance.
    Depositor
    Ian Duncan
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    lac
    Available Since
    Oct. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    160230-plasmid-map-sequence-id-314765

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  20. YARS2_pNIC-CTB10H

    Plasmid
    #153060
    Purpose
    Expression construct for producing Avi tagged Tyrosyl-tRNA synthetase 2
    Depositor
    Susanne Gräslund
    Insert
    YARS2 (YARS2 Human)
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153060-plasmid-map-sequence-id-304480

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Showing: 11681 - 11700 of 60327 results