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p358_DG

Plasmid

#234701

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-bdNEDD8

Depositor

Matthew Wohlever

Publication

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-bdNEDD8

Expression

Bacterial

Mutation

No

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p360_DG

Plasmid

#234703

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-mNeongreen-bdNEDD8

Depositor

Matthew Wohlever

Publication

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-mNeongreen-bdNEDD8

Expression

Bacterial

Mutation

No

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p355_DG

Plasmid

#234698

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-Thrombin

Depositor

Matthew Wohlever

Publication

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-Thrombin

Expression

Bacterial

Mutation

No

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-CasRx-non-targeting (pEZ18)

Plasmid

#229049

Purpose

Cloning backbone for CasRx crRNA (U6 promoter)

Depositor

S Chul Kwon

Publication

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-PspCas13b-non-targeting (pJK5)

Plasmid

#229052

Purpose

Cloning backbone for PspCas13b crRNA (U6 promoter)

Depositor

S Chul Kwon

Publication

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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p155ME-loxP-dsR-loxP-Cas9-t2A-GFP

Plasmid

#227774

Purpose

Middle-entry vector with dsRed flanked by loxP sites and spCas9-GFP for Gateway cloning

Depositor

Bushra Raj

Publication

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Insert

loxP-dsR-loxP

Use

CRISPR

Available since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pgRNA_AT

Plasmid

#196243

Purpose

Backbone plasmid for cloning sgRNA

Depositor

Andrea Puhar

Publication

Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR/zeo-MACROD1

Plasmid

#172572

Purpose

For gateway cloning of N-terminal tagged MACROD1 constructs.

Depositor

Karla Feijs

Publication

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD1 (MACROD1 Human)

Use

Gateway Cloning

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pADH1-GW

Plasmid

#209372

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. MCS upstream of gateway, driven by ADH1 promoter

Depositor

Lucia Strader

Publication

Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

Type

Empty backbone

Expression

Plant

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPT-TAR C1

Plasmid

#141246

Purpose

Transformation Associated Recombination (TAR) cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 1

Depositor

Bogumil Karas

Publication

Cochrane et al Algal Research, 2020. 50, 102014

Insert

Mitochondrial Genome of Phaeodactylum tricornutum

Use

Synthetic Biology; Diatom expression

Expression

Bacterial and Yeast

Mutation

Minor additional mutations described in manuscript

Available since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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U6-TM2Emp-6T_iBlue

Plasmid

#200235

Purpose

Backbone for cloning short RNA triggers that activate iSBH-sgRNAs

Depositor

Tatjana Sauka-Spengler

Publication

Pelea et al bioRxiv 2023.05.08.539738

Type

Empty backbone

Expression

Mammalian

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMA3735

Plasmid

#184848

Purpose

gRNA cloning using blue-white screening for recombinants

Depositor

Mikhail Alexeyev

Publication

Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

Insert

LacZalpha

Use

CRISPR

Expression

Mammalian

Mutation

alpha-fragment of LacZ

Promoter

U6

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK086

Plasmid

#171358

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

P-ANtRNA[Arg21]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR/zeo-MACROD2

Plasmid

#172573

Purpose

For gateway cloning of N-terminal tagged MACROD2 constructs.

Depositor

Karla Feijs

Publication

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD2 (MACROD2 Human)

Use

Gateway Cloning

Available since

April 7, 2022

Availability

Academic Institutions and Nonprofits only

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p{ie1-DsRed::GW,attB}

Plasmid

#182173

Purpose

fluorescent reporter gene with intron containing Gateway cloning cassette

Depositor

David Stern

Publication

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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p{ie1-eGFP::GW,attB}

Plasmid

#182172

Purpose

fluorescent reporter gene with intron containing Gateway cloning cassette

Depositor

David Stern

Publication

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

eGFP

Expression

Insect

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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p{Mhc-F4-678-DsRed::GW,attB}

Plasmid

#182171

Purpose

fluorescent reporter gene with intron containing Gateway cloning cassette

Depositor

David Stern

Publication

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK063

Plasmid

#171335

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

dSpCas9(m4) (for fusion)

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK062

Plasmid

#171334

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

dSpCas9(m2) (for fusion)

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK061

Plasmid

#171333

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Publication

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

SpCas9 (for fusion)

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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