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LentiCRISPRv2bleo-Nectin-2

Plasmid

#170295

Purpose

A knock-out vector for the mouse Nectin-2

Depositor

Michiyuki Matsuda

Publication

Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

Insert

A gRNA targeting the mouse Nectin-2 gene.

Use

CRISPR and Lentiviral

Available since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2bleo-Necl-5

Plasmid

#170294

Purpose

A knock-out vector for the mouse Necl-5

Depositor

Michiyuki Matsuda

Publication

Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

Insert

A gRNA targeting the mouse Necl-5 gene.

Use

CRISPR and Lentiviral

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD-DiB3

Plasmid

#168802

Purpose

Fluorogen activating protein (FAP) DiB3

Depositor

Alexander Mishin

Publication

Bozhanova et al Chem Sci. 2017 Oct 1;8(10):7138-7142. do

Insert

Fluorogen activating protein DiB3

Tags

His-tag

Expression

Bacterial

Promoter

araBAD

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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KG#471

Plasmid

#110935

Purpose

Expresses proteins in a subset of neurons in C. elegans, including isolated expression in the DA9 cholinergic motor neuron in the tail

Depositor

Kenneth Miller

Publication

Edwards et al Genetics. 2015 Sep;201(1):91-116. doi: 1

Inserts

mig-13 promoter
unc-54 3' control region

Expression

Bacterial

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX304-ARF5-T161A-mNeonGreen

Plasmid

#162026

Purpose

Fast cycling ARF mutant

Depositor

David Bryant

Publication

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

ARF5 (ARF5 Human)

Use

Lentiviral

Mutation

T161A

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSWD55

Plasmid

#158618

Purpose

Short Fibronectin FRET standard

Depositor

Seth Childers

Publication

Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

Insert

Short FRET Standard

Expression

Bacterial

Promoter

Xylose

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSWD56

Plasmid

#158619

Purpose

Long Fibronectin FRET Standard

Depositor

Seth Childers

Publication

Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

Insert

Long FRET Standard

Expression

Bacterial

Promoter

Xylose

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pZA45

Plasmid

#158523

Purpose

In planta gene expression of Golden gate compatible N. benthamiana ZAR1-eGFP. Km plant selection marker.

Depositor

Sophien Kamoun

Publication

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-eGFP

Tags

eGFP

Expression

Plant

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pZA37

Plasmid

#158515

Purpose

In planta gene expression of Golden gate compatible N. benthamiana ZAR1-6xHA. Km plant selection marker.

Depositor

Sophien Kamoun

Publication

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-6xHA

Tags

6xHA

Expression

Plant

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pZA24

Plasmid

#158502

Purpose

In planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V/L17E. Km plant selection marker.

Depositor

Sophien Kamoun

Publication

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1

Expression

Plant

Mutation

L17E, D481V

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pZA29

Plasmid

#158507

Purpose

In planta gene expression of Golden gate compatible N. benthamiana ZAR1-4xMyc. Km plant selection marker.

Depositor

Sophien Kamoun

Publication

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1-4xMyc

Tags

4xMyc

Expression

Plant

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pTKhc-yibDp-GFP-β20cp6

Plasmid

#127060

Purpose

Low phosphate inducible circular permutation of GFP, which is a substrate of the lon protease

Depositor

Michael Lynch

Publication

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

GFPcp6-beta20

Tags

His tag

Expression

Bacterial

Promoter

yibD

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-ILVBLpA-G10C-C21G

Plasmid

#120207

Purpose

Human ILVBL poly(A) site reporter, stem loop mutant rescue

Depositor

David Bartel

Publication

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

ILVBL poly(A) site

Expression

Mammalian

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-TFE3pA-G9C-C19G

Plasmid

#120203

Purpose

Human TFE3 poly(A) site reporter, stem loop mutant rescue

Depositor

David Bartel

Publication

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

TFE3 poly(A) site

Expression

Mammalian

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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BII-B3HTK

Plasmid

#133389

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

Depositor

Blair Madison

Publication

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Expression

Mammalian

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS3_K227R_Keima

Plasmid

#127144

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3_Keima (RPS3 Human)

Use

Lentiviral

Mutation

K227R

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4.13/AUG(30)-FFLuc-3XFLAG

Plasmid

#127336

Purpose

Express truncated 3xFLAG tagged firefly luciferase (FFLuc) from AUG at codon 30

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

Firefly luciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

SV40

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4.13/AUG(59)-FFLuc-3XFLAG

Plasmid

#127337

Purpose

Express truncated 3xFLAG tagged firefly luciferase (FFLuc) from AUG at codon 59

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

Firefly luciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

SV40

Available since

June 21, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-shRPS6

Plasmid

#125780

Purpose

constitutive expression of a short-hairpin RNA targeting human RPS6 (RNAi positive control)

Depositor

Francisca Vazquez

Publication

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shRPS6 (RPS6 Human)

Use

RNAi

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pPLtetO-FtsB

Plasmid

#107559

Purpose

Plasmid for titrating synthesis of FtsB with doxycycline

Depositor

Uwe Sauer

Publication

Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

Insert

ftsB

Use

Synthetic Biology

Promoter

pLtetO

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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