We narrowed to 66,865 results for: TOR;

1. pEM040 [Multiome Perturb-seq sgRNA expression vector]

   Plasmid

   #224898

   Purpose

   Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA, engineered for high-efficiency ID assignment in Multiome Perturb-seq experiments

   Depositor

   Thomas Norman

   Article

   Metzner et al bioRxiv [Preprint]. 2024 Jul 27:2024.07.

   Inserts

   eGFP
   LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET His6 MBP TEV LIC cloning vector (2M-T)

   Plasmid

   #29708

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis, MBP, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 4-2

   Plasmid

   #177919

   Purpose

   Expresses ratiometric Squash-SAM sensor 4-2 as circular RNA in mammalian cells using the Tornado expression system.

   Depositor

   Samie Jaffrey

   Article

   Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

   Insert

   Tornado-F30-Broccoli-Squash-SAM-sensor 4-2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6+27

   Available since

   Feb. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 5-1

   Plasmid

   #177920

   Purpose

   Expresses ratiometric Squash-SAM sensor 5-1 as circular RNA in mammalian cells using the Tornado expression system.

   Depositor

   Samie Jaffrey

   Article

   Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

   Insert

   Tornado-F30-Broccoli-Squash-SAM-sensor 5-1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6+27

   Available since

   Feb. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cre-responsive Cas9 expressing transposon library vector

   Plasmid

   #164138

   Purpose

   Backbone for transposon stgRNA library construction (library 4) (SpCas9 expression is induced upon Cre-loxP recombination.)

   Depositor

   Hyongbum Kim

   Article

   Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6, EF-1a

   Available since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET His6 ProteinG TEV LIC cloning vector (1P)

   Plasmid

   #29660

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis, Protein G, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET His6 Sumo TEV LIC cloning vector (2S-T)

   Plasmid

   #29711

   Depositor

   Scott Gradia

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   6xHis, SUMO, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCV PIG (Puro IRES GFP empty vector)

   Plasmid

   #21654

   Purpose

   Retroviral backbone for cloning and gene expression. Select with puromycin or GFP.

   Depositor

   David Bartel

   Article

   Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   GFP and IRES

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. Ai162 (TIT2L-GC6s-ICL-tTA2) targeting vector

   Plasmid

   #114433

   Purpose

   Target a Cre-dependent GCaMP6s cassette and a tTA2 cassette into the mouse TIGRE locus

   Depositor

   Hongkui Zeng

   Article

   Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

   Insert

   GCaMP6s, tTA2
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   TRE2, CAG

   Available since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET MBP His6 LIC cloning vector (2Cc-T)

    Plasmid

    #37237

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET His6 NusA TEV LIC cloning vector (1N)

    Plasmid

    #29657

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, NusA, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. Ai148 (TIT2L-GC6f-ICL-tTA2) targeting vector

    Plasmid

    #114428

    Purpose

    Target a Cre-dependent GCaMP6f expression and a tTA2 cassette into the mouse TIGRE locus

    Depositor

    Hongkui Zeng

    Article

    Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

    Insert

    GCaMP6f
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    TRE2, CAG

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Ai139 (TIT2L-GFP-ICL-TPT) targeting vector

    Plasmid

    #114426

    Purpose

    Target a Cre-dependent GFP and a tdTomato-P2A-tTA2 expression cassette into the mouse TIGRE locus

    Depositor

    Hongkui Zeng

    Article

    Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

    Insert

    GFP, tdTomato, tTA2,
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    TRE2, CAG

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET His6 Mocr TEV LIC cloning vector (1O)

    Plasmid

    #29658

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, Mocr, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET His6 NusA TEV LIC cloning vector (2N-T)

    Plasmid

    #29709

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6-NusA-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET Trx His6 LIC cloning vector (2Tc-T)

    Plasmid

    #37239

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis and Thioredoxin (Trx)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBac Dual His6 TEV LIC cloning vector (5B)

    Plasmid

    #30122

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRetroX-PTuner DD-M.EcoGII-v5-Telomeric repeat-binding-factor1

    Plasmid

    #122084

    Purpose

    Express M.EcoGII fused to TRF1 with detabilization domain - inducible

    Depositor

    Laure Crabbe

    Article

    Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

    Insert

    DD-linker-M.EcoGII-V5-TERF1
    

    Use

    Retroviral

    Tags

    V5 tag on Nter of TERF1

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. MTK Lentivirus Multi Cassette Destination Vector (pAN2424)

    Plasmid

    #194221

    Purpose

    Backbone for constructing multi casette expression plasmids using the Mammalian Tookit

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRK5 TGF beta type I receptor (T202D) Flag

    Plasmid

    #14833

    Depositor

    Rik Derynck

    Article

    Feng et al J Biol Chem. 1996 May 31. 271(22):13123-

    Insert

    TGF beta type I receptor (Tgfbr1 Rat)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Threonine 202 mutated to aspartate. Constitutivel…

    Promoter

    Available since

    May 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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