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pBiT3.1-C_WDR31:1-367

Plasmid

#211326

Purpose

Mammalian expression of full-length human WDR31 isoform 2. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR31:M1-F367 (WDR31 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

isoform 2. 40-40: Missing

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_STRN:1-731

Plasmid

#211304

Purpose

Mammalian expression of full-length human STRN isoform 2. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

STRN:M1-V731 (STRN Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

isoform 2

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_FBXW12:1-394

Plasmid

#211282

Purpose

Mammalian expression of full-length human FBXW12 isoform 2. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

FBXW12 :M1-T394 (FBXW12 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

isoform 2

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_FBXW10:1-1061

Plasmid

#211281

Purpose

Mammalian expression of full-length human FBXW10 isoform 2. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

FBXW10:M1-I1061 (FBXW10 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

isoform 2. R275L annotated sequence conflict

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_WDR31:1-367

Plasmid

#211252

Purpose

Mammalian expression of full-length human WDR31 isoform 2. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR31:M1-F367 (WDR31 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

isoform 2. 40-40: Missing

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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piggyBac-rtTA (4th_Gen)-NFIB-SOX9-IRES-mKate2 (VK_1244)

Plasmid

#209082

Purpose

NFIB and SOX9 mediated astrocye induction

Depositor

Vikram Khurana

Article

Lam et al Neuron. 2024 Sep 4;112(17):2886-2909.e16

Inserts

NFIB-P2A-SOX9-V5 (NFIB, SOX9 Human)
mKate2

Expression

Mammalian

Promoter

TRE4G

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_PLCG1:1-1291

Plasmid

#211089

Purpose

Mammalian expression of full-length human PLCG1 isoform 2. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

PLCG1:M1-L1291 (PLCG1 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

isoform 2. I813T natural variant (VAR_011908)

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_SCAP:1-905

Plasmid

#211228

Purpose

Mammalian expression of full-length human SCAP isoform 2. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

SCAP:M1-D905 (SCAP Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

isoform 2. V798I natural variant (VAR_012203)

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_SCAP:1-905

Plasmid

#211302

Purpose

Mammalian expression of full-length human SCAP isoform 2. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

SCAP:M1-D905 (SCAP Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

isoform 2. V798I natural variant (VAR_012203)

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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piggyBac-rtTA (4th_Gen)-SNCA(3K)-sfGFP-IRES-NGN2-puro (VK_1120)

Plasmid

#209078

Purpose

Ngn2 mediated cortical neuron induction, along with SNCA(3K) over-expression

Depositor

Vikram Khurana

Article

Lam et al Neuron. 2024 Sep 4;112(17):2886-2909.e16

Inserts

SNCA (SNCA Human)
NGN2 (Neurog2 Mouse)

Tags

sfGFP

Expression

Mammalian

Mutation

3K mutant: E35K, E46K, E61K

Promoter

TRE4G

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123)

Plasmid

#209080

Purpose

Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-sfGFP over-expression

Depositor

Vikram Khurana

Article

Lam et al Neuron. 2024 Sep 4;112(17):2886-2909.e16

Inserts

SNCA (SNCA Human)
NGN2 (Neurog2 Mouse)

Tags

sfGFP

Expression

Mammalian

Mutation

A53T

Promoter

TRE4G

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_PML:1-781

Plasmid

#211090

Purpose

Mammalian expression of full-length human PML isoform 13. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

PML:M1-L781 (PML Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

isoform 13 . E224D annotated sequence conflict. …

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_MED16:1-860

Plasmid

#211215

Purpose

Mammalian expression of full-length human MED16 isoform 3. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

MED16:M1-P860 (MED16 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

isoform 3

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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piggyBac-rtTA (4th_Gen)-SNCA(A53T-DNAC)-sfGFP-IRES-NGN2-puro (VK_1124)

Plasmid

#209081

Purpose

Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-DeltaNAC-sfGFP over-expression

Depositor

Vikram Khurana

Article

Lam et al Neuron. 2024 Sep 4;112(17):2886-2909.e16

Inserts

SNCA (SNCA Human)
NGN2 (Neurog2 Mouse)

Tags

sfGFP

Expression

Mammalian

Mutation

A53T, NAC deletion (aa60-95)

Promoter

TRE4G

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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CROPseq-SFFV-eBFP2

Plasmid

#239601

Purpose

Expresses either functional CRISPR gRNAs from the hU6-promotor and empowers pairing of CRISPR screens with 3' and 5' scRNA-Seq capturing by FACS-sorting on eBFP2+ without prior resistance selection

Depositor

Christian Krebs

Article

Enk et al Sci Immunol. 2024 Jun 14;9(96):eadd6774.

Insert

spleen focus‑forming virus - promotor

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-NRAS(G12D)-Neo

Plasmid

#232953

Purpose

Expresses mutant form of NRAS

Depositor

Jianjun Chen

Article

Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

Insert

NRAS(G12D)-Neo (NRAS Human)

Use

Lentiviral

Mutation

G12D

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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18065-M03-412

Plasmid

#225666

Purpose

Lentivirus protein expression. Control plasmid.

Depositor

Dominic Esposito

Article

Sharma et al Nat Commun. 2021 Dec 15;12(1):7300. doi:

Inserts

dsCopGFP
delCD19 (CD19 Human)

Use

Lentiviral

Tags

3xFLAG

Mutation

C-terminally truncated (aa 1-333 only)

Promoter

WPRE-SV40p and mCMVp

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-SFFV-PIK3CA H1047R-WPRE-UbC-Emerald

Plasmid

#203798

Purpose

Lentiviral vector expressing human PIK3CA H1047R under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

Depositor

Richard Eva, Bart Nieuwenhuis

Article

Nieuwenhuis et al EMBO Mol Med. 2020 Aug 7;12(8):e11674. d

Inserts

PIK3CA (PIK3CA Human)
Emerald

Use

Lentiviral

Expression

Mammalian

Mutation

H1047R

Available Since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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18065-M02-412

Plasmid

#225665

Purpose

Lentiviral expression of FLAG-tagged fluorescent proteins for immunohistochemical detection in FFPE tissue

Depositor

Dominic Esposito

Article

Sharma et al Nat Commun. 2021 Dec 15;12(1):7300. doi:

Inserts

dsmCherry
delCD19 (CD19 Human)

Use

Lentiviral

Tags

3xFLAG

Mutation

C-terminally truncated (aa 1-333 only)

Promoter

SORE6-mCMVp and WPRE-SV40p

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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18065-M04-412

Plasmid

#225667

Purpose

Lentivirus protein expression. Control plasmid.

Depositor

Dominic Esposito

Article

Sharma et al Nat Commun. 2021 Dec 15;12(1):7300. doi:

Inserts

dsmCherry
delCD19 (CD19 Human)

Use

Lentiviral

Tags

3xFLAG

Mutation

C-terminally truncated (aa 1-333 only)

Promoter

WPRE-SV40p and mCMVp

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge