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pNLRP11major.MYC

Plasmid

#131196

Purpose

Mammalian expression of human NLRP11 188S

Depositor

Thomas Kufer

Article

Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

Insert

NLRP11 188S (NLRP11 Human)

Tags

myc

Expression

Mammalian

Mutation

188S

Promoter

pCMV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry_eDHFR_SIM#1

Plasmid

#164646

Purpose

tag SIM with mCherry and eDHFR

Depositor

Michael Lampson, Huaiying Zhang

Article

Zhang et al Mol Biol Cell. 2020 Aug 15;31(18):2048-2

Insert

SIM

Use

Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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Dlx6

Plasmid

#15539

Depositor

John Rubenstein

Article

Liu et al Dev Dyn. 1997 Dec . 210(4):498-512.

Insert

Dlx6 (Dlx6 Mouse)

Expression

Bacterial

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
pMJ915v2+Nterm Spe1 +Cterm Age1 site

Plasmid

#88914

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCMX-PGC1

Plasmid

#6

Depositor

Bruce Spiegelman

Article

Puigserver et al Cell 1998 Mar 20;92(6):829-39.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Tags

Gal4

Expression

Mammalian

Available Since

June 27, 2005

Availability

Academic Institutions and Nonprofits only
pCRISPR_eGFP_314

Plasmid

#111822

Purpose

Knockout eGFP

Depositor

Steve Shih

Article

Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

Insert

gRNA targeting eGFP 294-314

Use

CRISPR

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-En2

Plasmid

#84310

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for En2. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

En2 (En2 Mouse)

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pHSP70B-CP6-TK

Plasmid

#165881

Purpose

Basic ganciclovir or acyclovir sensitvity cassette in Alpha2, lacks physical marker or attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Basic Selection Vector

Use

Synthetic Biology

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-RaACR_687-EYFP

Plasmid

#111077

Purpose

Anion channelrhodopsin from Rhodomonas abbreviata for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

Insert

Anion channelrhodopsin RaACR_687

Tags

EYFP

Expression

Mammalian

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-mROCK1-delta4

Plasmid

#101292

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Expression

Mammalian

Mutation

76-1354 aa

Promoter

CMV

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pHSP70B-CP6-pac

Plasmid

#165877

Purpose

Basic Puromycin resistance cassette in Alpha1, lacks physical marker or attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Basic Selection Vector

Use

Synthetic Biology

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-Foxd3 in situ

Plasmid

#37098

Depositor

Patricia Labosky

Article

Labosky et al Mech Dev. 1998 Aug;76(1-2):185-90.

Insert

Foxd3 (Foxd3 Mouse)

Use

In situ hybridization probe

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pcF-1

Plasmid

#131023

Purpose

Single spacer array with spacer #1 for FnCas12a.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Insert

single spacer array for FnCas12a

Use

CRISPR

Expression

Bacterial

Promoter

PJ23119

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4.11 promPDE4D3 Ebox mut

Plasmid

#27304

Depositor

David E. Fisher

Article

Khaled et al Genes Dev. 2010 Oct 15. 24(20):2276-81.

Insert

prom PDE4D3 (PDE4D Human)

Use

Luciferase

Mutation

Ebox CACATG changed to GAGATG

Available Since

Jan. 20, 2011

Availability

Academic Institutions and Nonprofits only

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enAsCas12a-mSA

Plasmid

#182954

Purpose

Express enAsCas12a-mSA

Depositor

Wen Xue

Article

Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

Insert

enAsCas12a-mSA

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-RNF17L

Plasmid

#35413

Depositor

Jeremy Wang

Article

Pan et al Development. 2005 Sep;132(18):4029-39. E

Insert

Rnf17l (Rnf17 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-hEFA6A

Plasmid

#206412

Purpose

Mammalian expression of hEFA6A

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Ueda et al FEBS Lett. 2013 Jun 5;587(11):1617-23. d

Insert

EFA6A (PSD Human)

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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mCerulean3-PMP-N-10

Plasmid

#55439

Purpose

Localization: Peroxisomes, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

PMP (PXMP2 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
pET/LEGH

Plasmid

#108226

Purpose

Bacterial expression of EGFP with N-terminal lanthanide binding tag and C-terminal His6 tag

Depositor

Didier Picard

Article

Bhattacharya et al Sci Rep. 2018 Feb 12;8(1):2801. doi: 10.

Insert

LBT-EGFP-His6

Expression

Bacterial

Promoter

T7, lacO

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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SNV-gfp-IRES-MCS

Plasmid

#53122

Purpose

Retrovirus vector backbone that co-expresses GFP and a gene of interest, driven by the Spleen Necrosis Virus promoter; for use in chicken cells

Depositor

Jeanette Hyer

Article

Venters et al Dev Dyn. 2008 Nov;237(11):3260-9. doi: 1

Type

Empty backbone

Use

Retroviral; For expression in chicken cells

Tags

EGFP-IRES

Promoter

SNV (spleen necrosis virus)

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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