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pCX-NTOM-SpiCee

Plasmid

#140902

Purpose

mammalian expression of NTOM-SpiCee, a mitochondria-targeted variant of the calcium buffer SpiCee that prevents downstream signaling

Depositor

Xavier Nicol

Publication

Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

Insert

NTOM-SpiCee

Tags

mRFP

Expression

Mammalian

Promoter

CAG

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pF3BGX-7xGFP11

Plasmid

#138397

Purpose

RMCE vector for generating C-terminal fusion of seven tandem GFP11 tags (split GFP)

Depositor

Shu Kondo

Publication

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

7xGFP11

Expression

Insect

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2 puro - CRBN (#2)

Plasmid

#166241

Purpose

sgRNA (#2) to generate a knockout of human CRBN by targeting the start region of Exon1

Depositor

Günter Schneider

Publication

Lier et al Bioorg Chem. 2021 Nov 20:105505. doi: 10

Insert

CRBN (CRBN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Dec. 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLEX307-HCoV-NL63-3CL

Plasmid

#160286

Purpose

Allows for constituitive mammalian expression of the HCoV-NL63 3CL protease via transfection or lentivirus

Depositor

Alejandro Chavez

Publication

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pSN001 pME minprom_mCerulean

Plasmid

#195959

Purpose

Gateway pME fluorophore

Depositor

Alexa Burger, Christian Mosimann

Publication

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

mouse beta-globin minimal promoter mCerulean

Use

Other

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCK011 p5E exorh

Plasmid

#195949

Purpose

Gateway p5E vector

Depositor

Christian Mosimann

Publication

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

exorh regulatory elements

Use

Other

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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766 p3E-2A-nlsmCherrypA

Plasmid

#195972

Purpose

Gateway p3E 2A fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Publication

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-nlsmCherry (nuclear)

Use

Other

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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768 pME mApple-CAAX

Plasmid

#195961

Purpose

Gateway pME fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Publication

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

mApple-CAAX (membrane)

Use

Other

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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764 pME nls-mApple

Plasmid

#195962

Purpose

Gateway pME fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Publication

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

mApple nuclear

Use

Other

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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PG13-mCherry

Plasmid

#195291

Purpose

mCherry reporter containing 13 copies of the p53-binding consensus sequence

Depositor

Hideyuki Oguro

Publication

Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

Insert

mCherry

Expression

Mammalian

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR_zeo_sgNT135

Plasmid

#183181

Purpose

pLentiCRISPR that is selectable with zeocin with a non-targeting sgRNA

Depositor

William Kaelin

Publication

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

NT135

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-monSTIM1

Plasmid

#161795

Purpose

Mammalian expression plasmid of monSTIM1 (ultra-photosensitive optogenetically-activated STIM1 protein)

Depositor

Won Do Heo

Publication

Kim et al Nat Commun. 2020 Jan 10;11(1):210. doi:

Insert

MonSTIM1 (STIM1 Human, Mustard Weed)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pMZ0002

Plasmid

#102239

Purpose

Expresses Chinese hamster NSF in bacteria

Depositor

Axel Brunger

Publication

Zhao et al Nature. 2015 Feb 5;518(7537):61-7. doi:

Insert

N-ethylmaleimide sensitive factor

Tags

6x His tag

Expression

Bacterial

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSP-G2

Plasmid

#64737

Purpose

Yeast expression plasmid

Depositor

Jens Nielsen

Publication

Partow et al Yeast. 2010 Nov;27(11):955-64. doi: 10.1

Type

Empty backbone

Expression

Yeast

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-G-Cdc42

Plasmid

#180577

Purpose

ddGFP sensor of Cdc42

Depositor

Won Do Heo

Publication

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Cdc42

Expression

Mammalian

Available since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAG364-greenFAST

Plasmid

#160353

Purpose

expresses greenFAST in mammalian cells

Depositor

Arnaud Gautier

Publication

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

greenFAST

Expression

Mammalian

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-DEPTOR-WT

Plasmid

#159159

Purpose

Expresses DEPTOR in mammalian cells

Depositor

Michele Pagano

Publication

Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

Insert

DEPTOR (DEPTOR Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pK263.CAG-loxP-stop-loxP-NLSturboRFP-ires-tTA-WPRE

Plasmid

#89587

Purpose

For nucleus-specific turboRFP labeling in single cells, pK263 should be used with pK031.

Depositor

Takuji Iwasato

Publication

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

nuclear localization signal (nls) turboRFP

Expression

Mammalian

Available since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLIB-TetR-KRAB-IRES-BLAS

Plasmid

#55820

Purpose

retroviral bicistronic expression vector for TetR-KRAB and Blasticidin S resistance

Depositor

Stephan Geley

Publication

Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

Insert

TetR-KRAB

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available since

Nov. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt16-V5

Plasmid

#35942

Depositor

Marian Waterman

Publication

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt16 (WNT16 Human)

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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