Addgene

We narrowed to 209 results for: prion

Showing: 101 - 120 of 209 results

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p415TEF-NYFP

Plasmid

#99555

Purpose

Expresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].

Depositor

Liming Li

Article

Hines et al PLoS Genet. 2011 Feb;7(2):e1001309. doi:

Insert

Swi1 N-rich region (AA1-327) (SWI1 Budding Yeast)

Use

Tags

YFP

Expression

Yeast

Mutation

Promoter

TEF1

Available since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pRS416 Gal-RNQ1 L94A-YFP

Plasmid

#18692

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 L94A (RNQ1 Budding Yeast)

Use

Tags

YFP

Expression

Yeast

Mutation

Leucine 94 mutated to Alanine (L94A)

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pJC25R1+4stop

Plasmid

#17723

Depositor

Susan Lindquist

Article

Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

Insert

Sup35R1+4 (SUP35 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

E.coli expression plasmid for NM-region of Sup35 …

Promoter

Available since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

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pDEST17-His10-ccdB

Plasmid

#166145

Purpose

Gateway destination vector for generation of N-terminal His10-tagged proteins in E. coli.

Depositor

Daniel Jarosz

Article

Itamar Harel et al BioRxiv

Type

Empty backbone

Use

Tags

His10

Expression

Bacterial

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pGAN201

Plasmid

#129204

Purpose

yTRAP sensor of [RNQ+], detects aggregation of Rnq1

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

RNQ1 (RNQ1 Budding Yeast, Synthetic)

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

SUP35

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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cytosolic moPrP (3F4)

Plasmid

#1321

Depositor

Susan Lindquist

Article

Ma et al Proc Natl Acad Sci U S A 2001 Dec 18;98(

Insert

PrP (Prnp Mouse)

Use

Tags

Expression

Mammalian

Mutation

N-term truncation (to make gene cytosolic), vario…

Promoter

Available since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only
pSK226

Plasmid

#129268

Purpose

dual yTRAP, RNQ1 fusion to aTF2 reporting on [RNQ+] state with mNeonGreen reporter

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

Rnq1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pRS306Sup35R1

Plasmid

#17715

Depositor

Susan Lindquist

Article

Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

Insert

pRS306Sup35R1 (SUP35 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

An pRS306 based integrating plasmid designed for …

Promoter

Available since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

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pWJPrP1

Plasmid

#22109

Depositor

Susan Lindquist

Article

Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

Insert

mouse PrP ORF insert (Prnp Mouse)

Use

Construction of targeting vector

Tags

Expression

Mutation

mutations at aa 108 and 111 to make 3F4 epitope (…

Promoter

Available since

Oct. 16, 2009

Availability

Academic Institutions and Nonprofits only
pWJPrP5

Plasmid

#22106

Depositor

Susan Lindquist

Article

Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

Insert

mouse PrP ORF insert (Prnp Mouse)

Use

Construction of targeting vector

Tags

Expression

Mutation

mutated codon 177 (D to N)

Promoter

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only
pAG415GPD-EGFP

Plasmid

#166137

Purpose

EGFP expression under the control of the GPD promoter yeast

Depositor

Daniel Jarosz

Article

Itamar Harel et al BioRxiv

Insert

EGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

GDP

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAG415GPD-mRuby3

Plasmid

#166138

Purpose

mRuby3 expression under control of the GPD promoter in yeast

Depositor

Daniel Jarosz

Article

Itamar Harel et al BioRxiv

Insert

mRuby3

Use

Tags

Expression

Yeast

Mutation

Promoter

GDP

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pX335_HR_Prnp_3

Plasmid

#78622

Purpose

pX335 vector encoding SpCas9n and a chimeric guide RNA targeting Prnp coding sequence

Depositor

Walker Jackson

Article

Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

Insert

HR_Prnp_3 (Prnp Mouse)

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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sgRNA(MS2)_Prnp_SAM2

Plasmid

#78625

Purpose

Encodes sgRNA2.0 targeting 138nt upstream of TSS

Depositor

Walker Jackson

Article

Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

Insert

sgRNA(MS2)_Prnp_SAM2 (Prnp Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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sgRNA(MS2)_Prnp_SAM1

Plasmid

#78624

Purpose

Encodes sgRNA2.0 targeting 158nt upstream of TSS

Depositor

Walker Jackson

Article

Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

Insert

sgRNA(MS2)_Prnp_SAM2 (Prnp Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-EGFP

Plasmid

#166140

Purpose

EGFP expression under the control of the Galactose-inducible promoter yeast. Contains CEN/ARS element for low copy within yeast.

Depositor

Daniel Jarosz

Article

Itamar Harel et al BioRxiv

Insert

EGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-EGFP

Plasmid

#166143

Purpose

EGFP expression under the control of the Galactose-inducible promoter in yeast. Contains 2 um element for high copy within yeast.

Depositor

Daniel Jarosz

Article

Itamar Harel et al BioRxiv

Insert

EGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-mRuby3

Plasmid

#166144

Purpose

mRuby3 expression under the control of the Galactose-inducible promoter in yeast. Contains 2 um element for high copy within yeast.

Depositor

Daniel Jarosz

Article

Itamar Harel et al BioRxiv

Insert

mRuby3

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pYES2/103Q

Plasmid

#1385

Depositor

Michael Sherman

Article

Meriin et al J Cell Biol 2002 Jun 10;157(6):997-1004.

Insert

huntingtin (103Q) (HTT Human)

Use

Tags

EGFP and FLAG

Expression

Yeast

Mutation

Exon1 sequences containing the first 17 amino aci…

Promoter

Available since

Feb. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pYES2/25Q

Plasmid

#1384

Depositor

Michael Sherman

Article

Meriin et al J Cell Biol 2002 Jun 10;157(6):997-1004.

Insert

huntingtin (25Q) (HTT Human)

Use

Tags

EGFP and FLAG

Expression

Yeast

Mutation

Exon1 sequences containing the first 17 amino aci…

Promoter

Available since

Feb. 12, 2009

Availability

Academic Institutions and Nonprofits only

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