We narrowed to 214 results for: prion

1. pRS416 Gal-RNQ1 L94A-YFP

   Plasmid

   #18692

   Depositor

   Susan Lindquist

   Article

   Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

   Insert

   Rnq1 L94A (RNQ1 Budding Yeast)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   Leucine 94 mutated to Alanine (L94A)

   Promoter

   Available since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

2. pJC25R1+4stop

   Plasmid

   #17723

   Depositor

   Susan Lindquist

   Article

   Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

   Insert

   Sup35R1+4 (SUP35 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E.coli expression plasmid for NM-region of Sup35 …

   Promoter

   Available since

   April 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGAN201

   Plasmid

   #129204

   Purpose

   yTRAP sensor of [RNQ+], detects aggregation of Rnq1

   Depositor

   Ahmad Khalil

   Article

   Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

   Insert

   RNQ1 (RNQ1 Budding Yeast, Synthetic)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SUP35

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. cytosolic moPrP (3F4)

   Plasmid

   #1321

   Depositor

   Susan Lindquist

   Article

   Ma et al Proc Natl Acad Sci U S A 2001 Dec 18;98(

   Insert

   PrP (Prnp Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N-term truncation (to make gene cytosolic), vario…

   Promoter

   Available since

   Jan. 21, 2005

   Availability

   Academic Institutions and Nonprofits only

5. pSK226

   Plasmid

   #129268

   Purpose

   dual yTRAP, RNQ1 fusion to aTF2 reporting on [RNQ+] state with mNeonGreen reporter

   Depositor

   Ahmad Khalil

   Article

   Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

   Insert

   Rnq1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS306Sup35R1

   Plasmid

   #17715

   Depositor

   Susan Lindquist

   Article

   Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

   Insert

   pRS306Sup35R1 (SUP35 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   An pRS306 based integrating plasmid designed for …

   Promoter

   Available since

   April 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWJPrP1

   Plasmid

   #22109

   Depositor

   Susan Lindquist

   Article

   Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

   Insert

   mouse PrP ORF insert (Prnp Mouse)
   

   Use

   Construction of targeting vector

   Tags

   Expression

   Mutation

   mutations at aa 108 and 111 to make 3F4 epitope (…

   Promoter

   Available since

   Oct. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pWJPrP5

   Plasmid

   #22106

   Depositor

   Susan Lindquist

   Article

   Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

   Insert

   mouse PrP ORF insert (Prnp Mouse)
   

   Use

   Construction of targeting vector

   Tags

   Expression

   Mutation

   mutated codon 177 (D to N)

   Promoter

   Available since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pX335_HR_Prnp_3

   Plasmid

   #78622

   Purpose

   pX335 vector encoding SpCas9n and a chimeric guide RNA targeting Prnp coding sequence

   Depositor

   Walker Jackson

   Article

   Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

   Insert

   HR_Prnp_3 (Prnp Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. sgRNA(MS2)_Prnp_SAM2

    Plasmid

    #78625

    Purpose

    Encodes sgRNA2.0 targeting 138nt upstream of TSS

    Depositor

    Walker Jackson

    Article

    Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

    Insert

    sgRNA(MS2)_Prnp_SAM2 (Prnp Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. sgRNA(MS2)_Prnp_SAM1

    Plasmid

    #78624

    Purpose

    Encodes sgRNA2.0 targeting 158nt upstream of TSS

    Depositor

    Walker Jackson

    Article

    Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

    Insert

    sgRNA(MS2)_Prnp_SAM2 (Prnp Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pYES2/103Q

    Plasmid

    #1385

    Depositor

    Michael Sherman

    Article

    Meriin et al J Cell Biol 2002 Jun 10;157(6):997-1004.

    Insert

    huntingtin (103Q) (HTT Human)
    

    Use

    Tags

    EGFP and FLAG

    Expression

    Yeast

    Mutation

    Exon1 sequences containing the first 17 amino aci…

    Promoter

    Available since

    Feb. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST17-His10-ccdB

    Plasmid

    #166145

    Purpose

    Gateway destination vector for generation of N-terminal His10-tagged proteins in E. coli.

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Type

    Empty backbone

    Use

    Tags

    His10

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYES2/25Q

    Plasmid

    #1384

    Depositor

    Michael Sherman

    Article

    Meriin et al J Cell Biol 2002 Jun 10;157(6):997-1004.

    Insert

    huntingtin (25Q) (HTT Human)
    

    Use

    Tags

    EGFP and FLAG

    Expression

    Yeast

    Mutation

    Exon1 sequences containing the first 17 amino aci…

    Promoter

    Available since

    Feb. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PRNP-bio-His

    Plasmid

    #51811

    Purpose

    Expresses full-length Major prion protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    PRNP (PRNP Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGAN200

    Plasmid

    #129203

    Purpose

    yTRAP sensor of [PSI+], detects aggregation of Sup35

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Insert

    Sup35NM (SUP35 Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    N terminal and middle domains

    Promoter

    SUP35

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSK267

    Plasmid

    #129272

    Purpose

    "ZF only" yTRAP control to control for yTRAP reporter fluorescence changes not dependent on sensed protein

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Insert

    NLS-VP16-ZF 43-8-NES
    

    Use

    Tags

    6xHA

    Expression

    Yeast

    Mutation

    Promoter

    SUP35

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX459_HR_Prnp_3

    Plasmid

    #78623

    Purpose

    pX459 vector encoding SpCas9-2A-Puro and a chimeric guide RNA targeting Prnp coding sequence

    Depositor

    Walker Jackson

    Article

    Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

    Insert

    HR_Prnp_3 (Prnp Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221-DDX5

    Plasmid

    #166163

    Purpose

    Gateway entry vector for N. furzeriDDX5

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Insert

    DDX5
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR221-mRuby3

    Plasmid

    #166136

    Purpose

    Gateway entry vector for mRuby3

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Insert

    mRuby3
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge