We narrowed to 106 results for: 62988

1. pSpCas9(BB)-2A-Puro (PX459) V2.0

   Plasmid

   #62988

   Purpose

   Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9-2A-Puro V2.0
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. pX461-ABE8e-nSpCas9

   Plasmid

   #237460

   Purpose

   Transfection-based all-in-one base editor plasmid that expresses both adenine base editor (ABE8e-nSpCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA.

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

   Insert

   3xFlag_ABE8e_SpCas9(D10A)_6xNLS
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   chicken beta-actin promoter & U6

   Available Since

   Jan. 12, 2026

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX461-evoCDA1-nSpCas9

   Plasmid

   #237461

   Purpose

   Transfection-based all-in-one base editor plasmid that expresses both cytosine base editor (evoCDA1-nSpCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA.

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

   Insert

   3xFlag_evoCDA1_ssDBD_nickase SpCas9 _6xUGI
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   chicken beta-actin promoter & U6

   Available Since

   Jan. 12, 2026

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX462-ABE8e-nSaCas9

   Plasmid

   #237462

   Purpose

   Transfection-based all-in-one base editor plasmid that expresses both adenine base editor (ABE8e-nSaCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA.

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

   Insert

   3xFlag_ABE8e_SaCas9 -6xNLS
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   chicken beta-actin promoter & U6

   Available Since

   Jan. 12, 2026

   Availability

   Academic Institutions and Nonprofits only

   

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4. PX459-CTNNA1(α-catenin CRISPR KO)

   Plasmid

   #229708

   Purpose

   Knockout of a-catenin in mammalian cells using CRISPR-Cas9 technology

   Depositor

   Alpha Yap

   Article

   Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

   Insert

   CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX459-sgAAVS1

   Plasmid

   #184403

   Purpose

   pX459 (sgRNA and Cas9 expressing plasmid, RRID:Addgene_62988) with targeting sequence specific to AAVS1; targeting sequence is: GGGGCCACTAGGGACAGGAT

   Depositor

   Robert Bradley

   Article

   Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

   Insert

   AAVS1-specific sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 promoter

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-U6-sgRNA_NUDT5_ex4-EF1α-Cas9-NLS-FLAG-GSG-P2A-PuroR-WPRE

   Plasmid

   #250374

   Purpose

   Lentiviral expression of sgRNA against human NUDT5 exon 4 under U6 promoter and Cas9-NLS-FLAG-GSG-P2A-PuroR under EF1α core promoter.

   Depositor

   Stefan Kubicek

   Article

   Nguyen et al Science. 2025 Nov 6:eadv4257. doi: 10.11

   Insert

   sgNUDT5 (NUDT5 Human)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   Feb. 3, 2026

   Availability

   Academic Institutions and Nonprofits only

   

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