-
Plasmid#140036PurposeKO of PCC 11901 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoterDepositorInsertTruncated thioesterase gene
UseTagsExpressionBacterialMutationPromotercLac143Available sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPPi-30m
Plasmid#13556DepositorInsertFLIPPi T22A
UseTagsECFP and EYFPExpressionBacterialMutationT22A in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPPi-200u
Plasmid#13555DepositorInsertFLIPPi G162A
UseTagsECFP and EYFPExpressionBacterialMutationG162A in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPPi-5u
Plasmid#13554DepositorInsertFLIPPi S52A
UseTagsECFP and EYFPExpressionBacterialMutationS52A in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPPi-260n
Plasmid#13552DepositorInsertFLIPPi WT
UseTagsECFP and EYFPExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pC1.530
Plasmid#205441PurposeLevel 1 Position 1 pUC19 vector with sgRNA and GmR. Used for homologous recombination with Cas12a editing vector pC1.509. sgRNA targets pC1.509 for removal.DepositorInsertRepB gRNA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSZT025
Plasmid#140033PurposeKO of PCC 7002 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoterDepositorInsertTruncated thioesterase gene
UseTagsExpressionBacterialMutationPromotercLac143Available sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCas5
Plasmid#82378PurposesgRNA targeting YFP expressed from pJ23119 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPJ23119Available sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPPi-4u
Plasmid#13553DepositorInsertFLIPPi T163A
UseTagsECFP and EYFPExpressionBacterialMutationT163A in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCas2F
Plasmid#82377PurposedCas9 expressed from aTc-inducible promoter in acsA, F RBS: TGGGCGDepositorInsertdCas9
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas2D
Plasmid#82375PurposedCas9 expressed from aTc-inducible promoter in acsA, D RBS: AGGACGDepositorInsertdCas9
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas61
Plasmid#82397PurposeIPTG inducible, cLac143 (Markley 2015), LDH V39R with Gmr in glpKDepositorInsertLDH
UseTagsExpressionMutationV39RPromotercLac143Available sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
STREP-SUMO-FLAG-SeKaiC
Plasmid#218179PurposeWT SeKaiC for phosphorylation/dephosphorylation studyDepositorInsertSTREP-SUMO-FLAG-SeKaiC
UseTagsSTREP-TagExpressionBacterialMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0.425
Plasmid#203944PurposeUp Flank. Up flanking region of RSF1010-Cas12a vector for introducing gRNA expression cassette and swapping out the AbR.DepositorInsertCas12a UP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0.426
Plasmid#203945PurposeDown Flank. Down flanking region of RSF1010-Cas12a vector for introducing gRNA expression cassette and swapping out the AbR.DepositorInsertCas12a DOWN
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0.391
Plasmid#203948PurposeLevel 0 part. Cas12a coding sequence from Francisella novicida in CDS1 positionDepositorInsertFnCas12a
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCas2B
Plasmid#82373PurposedCas9 expressed from aTc-inducible promoter in acsA, B RBS: TCGAGADepositorInsertdCas9
UseTagsExpressionMutationPromoterPEZ3Available sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas8
Plasmid#82380PurposedCas9 without a promoter in acsADepositorInsertdCas9
UseTagsExpressionMutationPromoternoneAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas7
Plasmid#82379PurposedCas9 expressed from low constituve promoter, c225, in acsADepositorInsertdCas9
UseTagsExpressionMutationPromoterc225Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas2E
Plasmid#82376PurposedCas9 expressed from aTc-inducible promoter in acsA, E RBS: AGGGCGDepositorInsertdCas9
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only