PurposeCyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 3 (NS3).
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||120083||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4705
Modifications to backbonePlasmid assembled from 3 CYANO-VECTOR modular devices: pCVD025 / CV-S7942NS3-RK2BOM: The device comprises the chromosome integration site NSIII for Synechococcus elongatus PCC7942. The device has an origin of replication for E. coli and a bom site for conjugal transfer, which are derived from pBR322. The device also comprises a Tetracycline resistance marker. pCVD001 / CV-aacC1: The device confers gentamicin (Gm) resistance. pCVD015 / CV-ccdB-SwaI: The device comprises the ccdB gene (toxic for commonly used E. coli cell line), which can be taken out with the SwaI endonuclease and swapped for the sequence(s) of interest using adaptors optimized for seamless assembly reaction.
Growth in Bacteria
Bacterial Resistance(s)Gentamicin, 10 μg/mL
Growth Strain(s)ccdB Survival
Gene/Insert namePlasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS3-RK2BOM, CV-aacC1, CV-ccdB-SwaI
SpeciesSynechococcus elongatus PCC 7942
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAM4941 (pCV0053) was a gift from Susan Golden (Addgene plasmid # 120083 ; http://n2t.net/addgene:120083 ; RRID:Addgene_120083)
For your References section:Broad-host-range vector system for synthetic biology and biotechnology in cyanobacteria. Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW. Nucleic Acids Res. 2014 Jul 29. pii: gku673. 10.1093/nar/gku673 PubMed 25074377