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pAM4937 (pCV0049)
(Plasmid #120082)


Item Catalog # Description Quantity Price (USD)
Plasmid 120082 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 4372
  • Modifications to backbone
    Plasmid assembled from 3 CYANO-VECTOR modular devices: pCVD021 / CV-S7942NS2: The device comprises the chromosome integration site NSII for Synechococcus elongatus PCC7942. The device has an origin of replication for E. coli and a bom site for conjugal transfer, which are derived from pBR322. pCVD003 / CV-aph1: The device confers kanamycin or neomycin (Km/Nm) resistance. pCVD015 / CV-ccdB-SwaI: The device comprises the ccdB gene (toxic for commonly used E. coli cell line), which can be taken out with the SwaI endonuclease and swapped for the sequence(s) of interest using adaptors optimized for seamless assembly reaction.
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    ccdB Survival
  • Copy number


  • Gene/Insert name
    Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS2, CV-aph1, CV-ccdB-SwaI
  • Species
    Synechococcus elongatus PCC 7942

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAM4937 (pCV0049) was a gift from Susan Golden (Addgene plasmid # 120082 ; ; RRID:Addgene_120082)
  • For your References section:

    Broad-host-range vector system for synthetic biology and biotechnology in cyanobacteria. Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW. Nucleic Acids Res. 2014 Jul 29. pii: gku673. 10.1093/nar/gku673 PubMed 25074377