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pCMV2 Bxb1-c10

Plasmid

#242872

Purpose

Mammalian expression of hyperactive Bxb1 Integrase mutant (I87L, E434G)

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

Bxb1 Integrase

Expression

Mammalian

Mutation

I87L, E434G

Promoter

CMV

Available Since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV2 Bxb1-c2

Plasmid

#242871

Purpose

Mammalian expression of hyperactive Bxb1 Integrase mutant (I87L)

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

Bxb1 Integrase

Expression

Mammalian

Mutation

I87L

Promoter

CMV

Available Since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV2 HuOpt Bxb1

Plasmid

#242869

Purpose

Mammalian expression of Bxb1 Integrase

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

Bxb1 Integrase

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pJBL3986

Plasmid

#204132

Purpose

Thermotoga petrophila (T. pe) fluoride riboswitch, wild type

Depositor

Julius Lucks

Article

Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

Insert

T. pe fluoride riboswitch sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pJBL811

Plasmid

#204135

Purpose

Low-copy constitutively expressed sfGFP for E. coli

Depositor

Julius Lucks

Article

Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

Insert

sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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POC1431

Plasmid

#221578

Purpose

Assembled plasmid containing four inserts (from POC1426, POC1427, POC1428 and POC1429) assembled using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

Assembled construct flanked by methylation-protectable BsaI sites and containing four always-methylable internal BsaI sites

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-G-iRFP720

Plasmid

#229177

Purpose

lvl 0 5 gene

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

iRFP720

Use

Synthetic Biology

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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lvl1-Adapter4

Plasmid

#229205

Purpose

lvl 1 adapter

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

S5-SX - adapter

Use

Synthetic Biology

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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Gal4-pcVP16-1

Plasmid

#231570

Purpose

Expresses the single component, light-off regulated pcVP16-1 transcriptional regulator

Depositor

Dominik Niopek

Article

Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

Insert

Gal4-pcVP16-1

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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lvl1-Adapter1

Plasmid

#229202

Purpose

lvl 1 adapter

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

S2-SX - adapter

Use

Synthetic Biology

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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lvl1-Adapter3

Plasmid

#229204

Purpose

lvl 1 adapter

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

S4-SX - adapter

Use

Synthetic Biology

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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lvl1-Adapter2

Plasmid

#229203

Purpose

lvl 1 adapter

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

S3-SX - adapter

Use

Synthetic Biology

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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lvl1-U6tetO_shFF4

Plasmid

#229207

Purpose

lvl 1 expresion vector

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

Pol3 expresion of shFF4

Use

Synthetic Biology

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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lvl1-Adapter5

Plasmid

#229206

Purpose

lvl 1 adapter

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

S6-SX - adapter

Use

Synthetic Biology

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-I-2xcHS4

Plasmid

#229160

Purpose

lvl 0 insulator

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

2xcHS4

Use

Synthetic Biology

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA47_dCas9-GFP_AAN_4

Plasmid

#231415

Purpose

This AND-AND-NOT-gate plasmid expresses dCas9 from a constitutive promoter. Its sgRNAs X & Y are repressible by sgRNAs A & B, respectively. Its GFP gene is repressible by any of sgRNAs X, Y, and C.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

dCas9
GFP
sgRNA-X
sgRNA-Y

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-I-inert

Plasmid

#229159

Purpose

lvl 0 insulator

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

inert

Use

Synthetic Biology

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_Zdk3-VPR_P2A_dCas9-AsLOVx3-NLSx3

Plasmid

#231568

Purpose

Expresses components of the LOOMINA optogenetic transcriptional control system.

Depositor

Dominik Niopek

Article

Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

Insert

All-in-one LOOMINA construct

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_dCas9-AsLOV2x3-NLSx3

Plasmid

#231567

Purpose

Expresses components of the LOOMINA optogenetic transcriptional control system.

Depositor

Dominik Niopek

Article

Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

Insert

dCas9-AsLOV2x3

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-G-eBFP2

Plasmid

#229182

Purpose

lvl 0 5 gene

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

eBFP2

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl1-Adapter1-SapI

Plasmid

#229200

Purpose

lvl 1 adapter

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

T2-T4 - Sap1 adapapter

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl1-Adapter2-SapI

Plasmid

#229201

Purpose

lvl 1 adapter

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

T3-T4 - Sap1 adapapter

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-G-mKate2

Plasmid

#229180

Purpose

lvl 0 5 gene

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

mKate2

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-G-Sirius

Plasmid

#229181

Purpose

lvl 0 5 gene

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

Sirius

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-3-inert

Plasmid

#229190

Purpose

lvl 0 3 prime UTR

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

inert

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-G-rtTA

Plasmid

#229189

Purpose

lvl 0 5 gene

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

rtTA

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-P-CMV

Plasmid

#229165

Purpose

lvl 0 promoter

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

CMV

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-G-inert

Plasmid

#229175

Purpose

lvl 0 5 gene

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

inert

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-P-Gal4B3Hybrid

Plasmid

#229167

Purpose

lvl 0 promoter

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

Gal4B3Hybrid

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-P-hUBC

Plasmid

#229164

Purpose

lvl 0 promoter

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

hUBC

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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