Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 1,763 results for: MB;

Showing: 1181 - 1200 of 1763 results

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pUPD3_FULLPRM_PR

Plasmid

#243916

Purpose

GoldenBraid2.0 Temperature-Inducible promoter, including ribosome binding site. Lacks repressor, cI857.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PR without cI857

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_PT7

Plasmid

#243917

Purpose

GoldenBraid2.0 T7 RNA polymerase-dependent promoter, including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PT7

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_PT7lac

Plasmid

#243918

Purpose

GoldenBraid2.0 T7 RNA polymerase and IPTG/Lactose-dependent promoter, including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PT7lac

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pBSKo1_Stuffer1_Stuffer2

Plasmid

#243896

Purpose

GoldenBraid2.0 Full transcription unit stuffer in an omega-level cloning vector.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

Stuffer1 and Stuffer2

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pBSKo2_Stuffer2_Stuffer1

Plasmid

#243897

Purpose

GoldenBraid2.0 Full transcription unit stuffer in an omega-level cloning vector.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

Stuffer2 and Stuffer1

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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ptetgRNA-FRT

Plasmid

#243872

Purpose

sgRNA expression vector under tetR transcriptional control.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

sgRNA_FRT

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pBSKa1_Stuffer1

Plasmid

#243844

Purpose

GoldenBraid2.0 Full transcription unit stuffer in an alpha-level cloning vector.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

Stuffer1

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pBSKa2_Stuffer2

Plasmid

#243847

Purpose

GoldenBraid2.0 Full transcription unit stuffer in an alpha-level cloning vector.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

Stuffer2

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 E3GFP

Plasmid

#233008

Purpose

Galactose iduced expression of Gcn4 E+GFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 E+

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

D105E, D118E, D139E

Promoter

Gal1

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_FL Gcn4

Plasmid

#232955

Purpose

Galactose iduced expression of full-length Gcn4 in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4

Tags

TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 SATtoG KtoRHA

Plasmid

#232994

Purpose

Galactose iduced expression of Gcn4 SATtoG KtoRHAin yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 SATtoG KtoR

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Aro3+HA

Plasmid

#232990

Purpose

Galactose iduced expression of Gcn4 Aro3+HA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro3+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

D103Y, N112T, L113Y, S117F, K140R

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 WT 44merHA

Plasmid

#232988

Purpose

Galactose iduced expression of Gcn4 WT 44merHA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WT 44mer

Tags

3xHA and TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Aro2+HA

Plasmid

#232989

Purpose

Galactose iduced expression of Gcn4 Aro2+HA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro2+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

N116F, S117E, E119N, P129Q, T132Y

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 WW+HA

Plasmid

#232991

Purpose

Galactose iduced expression of Gcn4 WW+HA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WW+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

E109N, S117D, T121W, N126W, T132W

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 SATtoG KtoR

Plasmid

#232961

Purpose

Galactose iduced expression of Gcn4 ATtoG KtoR in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 SATtoG KtoR

Tags

TEV cleavage site

Expression

Yeast

Mutation

S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG415Gal_Gcn4 WW+GFP

Plasmid

#233003

Purpose

Galactose iduced expression of Gcn4 WW+GFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WW+

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

E109N, S117D, T121W, N126W, T132W

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG415Gal_Gcn4 Aro2+GFP

Plasmid

#233001

Purpose

Galactose iduced expression of Gcn4 Aro2+GFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro2+

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

N116F, S117E, E119N, P129Q, T132Y

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 S/ArotoL

Plasmid

#232979

Purpose

Galactose iduced expression of Gcn4 S/ArotoL in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 S/ArotoL

Tags

TEV cleavage site

Expression

Yeast

Mutation

S101L, S104L, F108L, Y110L, S117L, S136L, S144L

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 ILVtoED solvvol

Plasmid

#232958

Purpose

Galactose iduced expression of Gcn4 LVtoED solvvol in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED solvvol

Tags

TEV cleavage site

Expression

Yeast

Mutation

S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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