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Showing: 1181 - 1200 of 1509 results

  8. pRSET.GltI253-cpGFP.L1LV/L2NP

    Plasmid
    #41733
    Purpose
    a single-wavelength intracellular glutamate sensor constructed from E. coli Gltl and cpGFP. For bacterial cytoplasmic expression
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Insert
    GltI based Glutamate Biosensor
    Use
    Glutamate biosensor
    Tags
    6xHis, T7 epitope, and Xpress tag
    Expression
    Bacterial
    Mutation
    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…
    Promoter
    T7
    Available Since
    Jan. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    41733-plasmid-map-sequence-id-56305

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  10. PDI-QUAD-V5-pcDNA3.1

    Plasmid
    #153550
    Purpose
    PDI-QUAD was generated from PDI-WT pcDNA3.1 (+) using two native active sites (CGHC, CGHC) tagged with V5, and mutated (to SGHS, SGHS) using site directed mutagenesis.
    Depositor
    Julie Atkin
    Article
    Parakh et al iScience. 2020 May 22;23(5):101097. doi:
    Insert
    Protein disulphide isomerase
    Tags
    V5 tag
    Mutation
    C53S, C56S, C397S, C400S
    Promoter
    pCMV
    Available Since
    Aug. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    153550-plasmid-map-sequence-id-308584

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  13. FcD265A-FLAG

    Plasmid
    #65420
    Purpose
    Mammalian expression plasmid for murine Fc-FLAG, used for monomeric Fc-IL2 production
    Depositor
    Dane Wittrup
    Article
    Zhu et al Cancer Cell. 2015 Apr 13;27(4):489-501.
    Insert
    FcD265A-FLAG
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D265A
    Promoter
    Modified CMV Promoter
    Available Since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. PhiC31-Neo-ins-5xTetO-pEF-H2B-Citrine-ins

    Plasmid
    #78099
    Purpose
    Nuclear fluorescent reporter in mammalian cells, phiC31 attB for integration, can be silenced with TetR-CR or rTetR-CR (e.g. CR can be KRAB or DNMT3B).
    Depositor
    Michael Elowitz
    Article
    Bintu et al Science. 2016 Feb 12;351(6274):720-4. do
    Insert
    Histone H2B - Citrine (H2BC21 Synthetic, Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    5xTetO pEF alpha
    Available Since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 1181 - 1200 of 1509 results