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1pBB3-ScVip1-536-1107-C793A

Plasmid

#238324

Purpose

1ScVip1-536-1107-C793A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-C793A

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-186-522

Plasmid

#238311

Purpose

ScVip1-186-522 protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-186-522

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-H548A

Plasmid

#238319

Purpose

ScVip1-536-1107-H548A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-H548A

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107

Plasmid

#238312

Purpose

ScVip1-536-1107 protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-K554A

Plasmid

#238329

Purpose

ScVip1-536-1107-K554A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-K554A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-R547A

Plasmid

#238318

Purpose

ScVip1-536-1107-R547A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-R547A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-R551A

Plasmid

#238320

Purpose

ScVip1-536-1107-R551A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-R551A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-E991A

Plasmid

#238321

Purpose

ScVip1-536-1107-E991A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-E991A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-H651A

Plasmid

#238323

Purpose

ScVip1-536-1107-H651A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-H651A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_Z11 gpN:SpyTag

Plasmid

#225196

Purpose

Used for adding C-terminal SpyTag to the Plesiomonas ZOR0011 P2 phage major capsid protein

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

gpN homology arms and SpyTag

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS∆tum

Plasmid

#225198

Purpose

Used to create a markerless deletion of the E. coli HS tum gene

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

tum homology arms

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS_lexA(Ind-)

Plasmid

#225199

Purpose

Used to create a non-inducible lexA by introducing a G85D mutation in E. coli HS

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

lexA homology arms

Expression

Bacterial

Mutation

lexA G85D

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS_gpN:SpyTag

Plasmid

#225193

Purpose

Used for adding C-terminal SpyTag to the E. coli HS P2 phage major capsid protein

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

gpN homology arms and SpyTag

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pEW66

Plasmid

#232794

Purpose

COMPASS Fragment 1 (Cps60, Cps50, Cps35, Cps25, Cps15) in PBIG1a

Depositor

Cynthia Wolberger

Article

Worden et al Elife. 2020 Jan 10;9. pii: 53199. doi: 1

Insert

COMPASS Fragment 1 (Cps60, Cps50, Cps35, Cps25, Cps15) (SWD1, BRE2, SWD2, SDC1, SHG1 Budding Yeast)

Tags

No tags

Expression

Insect

Promoter

polyhedrin promoter

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCIFR-6

Plasmid

#227665

Purpose

Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Gm) used for selecting for the integration can be removed in a later stage with pFNC.

Depositor

Pablo Ivan Nikel

Article

Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

Insert

msfGFP

Expression

Bacterial

Promoter

p14G-BCD2

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pFNC-6

Plasmid

#227669

Purpose

Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. GmR, easily curable via sucrose counterselection.

Depositor

Pablo Ivan Nikel

Article

Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

Inserts

BxbI phage integrase
sacB

Expression

Bacterial

Promoter

PEM7

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pACE SOX (ORF37) D221N E244Q

Plasmid

#192053

Purpose

Expresses a 2X catalytic mutant of (ORF37) D221N E244Q

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (D221N E244Q)

Use

Unspecified

Mutation

2X catalytic mutant D221N E244Q

Promoter

CMV

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pSG068

Plasmid

#214259

Purpose

pJN105 with PaftsH2SD and PaFtsH1 ORF cloned between NheI/XbaI

Depositor

Ute Romling

Article

Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

Insert

Core genome PaFtsH1 proteinase from Pseudomonas aeruginosa SG17M

Expression

Bacterial

Mutation

18 nucleotide 5' of the start codon (Shine-D…

Promoter

araBAD promoter

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pSC5GGv1_ShBle

Plasmid

#188603

Purpose

pSC5GGv1 with Sh ble insert for yeast selection

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Diatom expression

Expression

Bacterial and Yeast

Available Since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-BCAS2

Plasmid

#192294

Purpose

Gateway entry vector for an inducible 3xFLAG-BCAS2

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

BCAS2 (BCAS2 Human)

Use

Gateway Cloning

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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