Addgene

Showing: 5991 - 6000 of 78572 results

Results per page:

Sort results by:

pMSCV-hygro-STING HAQ

Plasmid

#102600

Purpose

Express STING HAQ for IFN-beta Luciferase reporter assay.

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

R71H-G230A-R293Q (HAQ)

Promoter

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-Hsp90 N(9-236)

Plasmid

#22481

Depositor

William Sessa

Article

Fontana et al Circ Res. 2002 May 3. 90(8):866-73.

Insert

Hsp90 alpha N domain (HSP90AA1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-nEF-Con/Fon-iC++-EYFP

Plasmid

#137155

Purpose

Intersectional viral expression of iC++-EYFP in cells expressing both Cre AND Flp

Depositor

INTRSECT 2.0 Project, Karl Deisseroth

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-iC++-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

n/a

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
BII-BCA-Prom-ASCL2

Plasmid

#133394

Purpose

Piggybac vector for heterologous promoter reporter assay of human ASCL2 promoter

Depositor

Blair Madison

Article

Strubberg et al Stem Cell Reports. 2018 Aug 14;11(2):410

Insert

destabilized tdTomato-NLS and destabilized NLS-GFP

Use

Tags

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free) drives expressi…

Available since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 MLL2 653

Plasmid

#11017

Depositor

Matthew Meyerson

Article

Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

Insert

MLL2 (KMT2B Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

truncation of MLL2, last 653 amino acids, from pr…

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pAcBac1-Mb-Fluoro-Phe D6

Plasmid

#197577

Purpose

Expression of Methanosarcina barkeri (Mb) Fluoro-Phe D6 tRNA synthetase/Pyl-tRNA pair for the encoding of fluorinated phenylalanine derivatives at TAG codons in HEK293 cells

Depositor

Ryan Mehl

Article

Galles et al Nat Commun. 2023 Jan 4;14(1):59. doi: 10

Inserts

Mb Pyl Fluoro-Phe B5 synthetase
Pyl-tRNA (4 copies)

Use

Tags

Nuclear export sequence + FLAG

Expression

Mammalian

Mutation

N311A, C313M, V366G, W382T

Promoter

CMV and U6/H1

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1+FLAG-KrasnatG12D

Plasmid

#206842

Purpose

To express mouse Kras encoded by native mouse codons and with a G12D mutation with an N-terminal FLAG tag.

Depositor

Christopher Counter

Article

Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

Insert

Kras (Kras Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Glycine 12 to Aspartic Acid

Promoter

Available since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-MIOS(1-875) in pRK5

Plasmid

#184561

Purpose

CMV-driven expression of an N-terminally Flag-tagged MIOS (core subunit of GATOR2) — native sequence for transient expression in mammalian cells.

Depositor

Kacper Rogala

Article

Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi:

Insert

MIOS (MIOS Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Ef1a-Coff/Fon-GCaMP6M

Plasmid

#137121

Purpose

Intersectional viral expression of GCaMP6M in cells expressing Flp AND NOT Cre

Depositor

INTRSECT 2.0 Project, Karl Deisseroth

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-GCaMP6M

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

Removed RSET tag

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Ef1a-Con/Fon-sRGECO

Plasmid

#137126

Purpose

Intersectional viral expression of sRGECO in cells expressing both Cre AND Flp

Depositor

INTRSECT 2.0 Project, Karl Deisseroth

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Fon-sRGECO

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

E217D

Promoter

EF1a

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge