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pLV-SV40-puro-EF1a-HA-NLGN1dAChE-bc

Plasmid

#220437

Purpose

Expresses HA-tagged human NLGN1 with K578A/V579A substitutions

Depositor

Paul Blainey

Article

Le et al Cell Rep. 2025 Jun 24;44(7):115889. doi:

Insert

Neuroligin-1 (NLGN1 Human)

Use

Lentiviral

Tags

HA-tag

Expression

Mammalian

Mutation

K578A and V579A substitutions

Promoter

EF1a

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-LbCpf1-crF1

Plasmid

#213046

Purpose

Guide RNA expression vector with the synthetic crRNA crF1 targeting the SWITCHER construct with the target site TF1

Depositor

Petr Bartunek

Article

Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

Insert

crF1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-LbCpf1-crR2

Plasmid

#213047

Purpose

Guide RNA expression vector with the synthetic crRNA crR2 targeting the SWITCHER construct with the target site TR2

Depositor

Petr Bartunek

Article

Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

Insert

crR2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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POC1426

Plasmid

#221573

Purpose

Plasmid containing the Insert 1 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1427

Plasmid

#221574

Purpose

Plasmid containing the Insert 2 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1428

Plasmid

#221575

Purpose

Plasmid containing the Insert 3 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1429

Plasmid

#221576

Purpose

Plasmid containing the Insert 4 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB027

Plasmid

#234636

Purpose

pET-28a(+) based plasmid for expression of H16_B1102 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_B1102 (H16_RS24270 )

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB028

Plasmid

#234637

Purpose

pET-28a(+) based plasmid for expression of H16_B1148 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_B1148 (H16_RS24495 )

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB029

Plasmid

#234638

Purpose

pET-28a(+) based plasmid for expression of H16_B1264 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_B1264 (H16_RS25065 )

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB030

Plasmid

#234639

Purpose

pET-28a(+) based plasmid for expression of H16_B1335 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_B1335 (H16_RS25400 )

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB024

Plasmid

#234633

Purpose

pET-28a(+) based plasmid for expression of H16_A0285 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_A0285 (H16_RS01400 Cupriavidus necator H16)

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB026

Plasmid

#234635

Purpose

pET-28a(+) based plasmid for expression of H16_A3514 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_A3514 (H16_RS17530 )

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA63_sgRNA-B0_M13ps

Plasmid

#231421

Purpose

M13 phagemid constitutively expressing sgRNA-B0. sgRNA-B0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ552.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-B0

Use

Synthetic Biology

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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P-Bf-fusA2

Plasmid

#235658

Purpose

Reports Cur activity in Bacteroides fragilis

Depositor

Guy Townsend

Article

Kabonick et al bioRxiv [Preprint]. 2024 Nov 13:2024.11.

Insert

Bacteroides fragilis fusA2 promoter

Mutation

The B. thetaiotaomicron fusA2 promoter lacking &q…

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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DriD-wHTH-DNABD

Plasmid

#237851

Purpose

The wHTH DNA binding domain used to understand the allosteric mechanism

Depositor

Maria Schumacher

Article

Cannistraci et al Commun Biol. 2025 Apr 29;8(1):679. doi:

Insert

DriD wHTH DNABD

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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DriD-3HB-mutant

Plasmid

#237852

Purpose

DriD 3HB surface mutant used to interrogate the allosteric mechanism

Depositor

Maria Schumacher

Article

Cannistraci et al Commun Biol. 2025 Apr 29;8(1):679. doi:

Insert

DriD 3HB mutant

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHK401

Plasmid

#235482

Purpose

Repression of gene VIII by Plux/tet hybrid promoter

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

phage M13 geneVIII, cI repressor

Expression

Bacterial

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pHK201

Plasmid

#235481

Purpose

Repression of gene VIII by Ptac promoter

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

phage M13 geneVIII, cI repressor

Expression

Bacterial

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pHK328

Plasmid

#235479

Purpose

Inducible gene VIII by Pbad/lac hybrid promoter

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

phage M13 geneVIII

Expression

Bacterial

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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