We narrowed to 22,307 results for: CRISPR

1. pCR1003

   Plasmid

   #71821

   Purpose

   Express Streptococcus pyogenes dCas9 (D10A, H840A)

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

   Insert

   T7pr_10xHis-MBP-TEV-SPydCas9 (D10A, H840A)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. peft-3::cas-9::tbb-2 3'UTR

   Plasmid

   #48960

   Purpose

   this plasmid contains the codon optimized gene encoding Cas9 for C. elegans.

   Depositor

   Mario de Bono

   Article

   Chen et al Nucleic Acids Res. 2013 Sep 5.

   Insert

   cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   eef-1A.1 (eft-3)

   Available since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEPOR0SP0013

   Plasmid

   #117521

   Purpose

   Level0 Golden Gate part: CDS, SpCas9-p

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   SpCas9-p (with stop codon)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMB67

   Plasmid

   #47947

   Purpose

   Expresses C. elegans optimized Cas9 from the hsp-16.48 promoter

   Depositor

   Mike Boxem

   Article

   Waaijers et al Genetics. 2013 Aug 26.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and egl-13 NLS

   Expression

   Worm

   Mutation

   Promoter

   hsp-16.48

   Available since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. PX458_GABPA_2

   Plasmid

   #64255

   Purpose

   Expresses gRNA against human GABPA along with Cas9 with 2A GFP

   Depositor

   Richard M. Myers, Eric Mendenhall

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. NAX_CAGGS_CAS9_EBFP2

   Plasmid

   #167857

   Purpose

   PiggyBac compatible plasmid expressing spCas9

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. PX458_GABPA_1

   Plasmid

   #64254

   Purpose

   Expresses gRNA against human GABPA along with Cas9 with 2A GFP

   Depositor

   Richard M. Myers, Eric Mendenhall

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJA50

   Plasmid

   #59931

   Purpose

   gRNA for cleavage at unc-58(e665) locus in C elegans

   Depositor

   Andrew Fire

   Article

   Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

   Insert

   unc-58(e665) gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Sept. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBLlSh2

   Plasmid

   #170981

   Purpose

   Expression of ShCAST under control of lacI Plac promoter/repressor pair.

   Depositor

   Christopher Reisch

   Article

   Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

   Inserts

   lacI
   ShTnsB, ShTnsC, ShTniQ, ShCas12k
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLlac01

   Available since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCR1055

    Plasmid

    #71823

    Purpose

    Express Streptococcus pyogenes nCas9 (H840A) nickase carrying two C-terminal SV40 NLS

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

    Insert

    T7pr_6xHis-MBP-TEV-SPynCas9 (H840A)-2xNLS
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAct-dCBEevoAPOBEC1

    Plasmid

    #195514

    Purpose

    Ubiquitous expression of dCBEevoAPOBEC1 in Drosophila

    Depositor

    Michael Boutros

    Article

    Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

    Insert

    dCBE-evoAPOBEC1
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    act5C

    Available since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSCBE3-HF

    Plasmid

    #133967

    Purpose

    Expresses HF-BE3 CRISPR base editor and gRNA scaffold in Streptomyces

    Depositor

    Yuhui Sun

    Article

    Zhong et al bioRxiv 630137

    Insert

    HF-BE3
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-GBP-dCas9-mRFP

    Plasmid

    #102933

    Purpose

    Expression construct for dCas9 fused to GFP-binding Protein (N-terminally) and mRFP (C-terminus)

    Depositor

    Sebastian Bultmann

    Article

    Anton et al Nucleus. 2017 May 4;8(3):279-286. doi: 1

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    GBP (GFP binding protein) and mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

14. NAX_CAGGS_CAS9_EYFP

    Plasmid

    #167858

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJA54

    Plasmid

    #60212

    Purpose

    cleavage near e1350 locus in sqt-1

    Depositor

    Andrew Fire

    Article

    Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

    Insert

    sqt-1(e1350) gRNA1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. PX458_ATF1_1

    Plasmid

    #64690

    Purpose

    Expresses gRNA against human ATF1 along with Cas9 with 2A GFP

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJA59

    Plasmid

    #59932

    Purpose

    gRNA for cleavage at unc-109(n499) locus in C elegans

    Depositor

    Andrew Fire

    Article

    Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

    Insert

    unc-109(n499) gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLdSaCN

    Plasmid

    #123261

    Purpose

    Expresses Staphylococcus aureus Cas9 (SaCas9) and its gRNA in Leishmania

    Depositor

    Greg Matlashewski

    Article

    Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e004

    Insert

    gRNA and SaCas9
    

    Use

    CRISPR; Leishmania

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. JME4393

    Plasmid

    #129657

    Purpose

    LYS5ex_CrisprCas9-yl_RFP: CAS9 vector with LYS5ex marker for gRNA cloning using GoldenGate

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJA55

    Plasmid

    #59935

    Purpose

    gRNA for cleavage near sqt-1(e1350) locus in C elegans

    Depositor

    Andrew Fire

    Article

    Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

    Insert

    sqt-1(e1350) gRNA2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAN-PBAD-sgRNA-scramble

    Plasmid

    #62285

    Purpose

    expression of scramble sgRNA from the arabinose-inducible promoter

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    scramble sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCF823_EFS-Cas9-PuroR_lenti

    Plasmid

    #138317

    Purpose

    Lenti expression of SpyCas9 with Puro selection

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    SpyCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSL1166

    Plasmid

    #129520

    Purpose

    pDEF-SpCas9::GFP, For deletion of pyalba4, PyBiP-full promoter, 2nd Generation

    Depositor

    Scott Lindner

    Article

    Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

    Insert

    SpCas9
    

    Use

    Plasmodium, rodent-infectious species

    Tags

    3XFLAG-NLS

    Expression

    Mutation

    Promoter

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pKEW321-MbCas12a-RFP-GFP-array

    Plasmid

    #115661

    Purpose

    Encodes an CRISPR array for MbCas12a targeting superfolder GFP and mRFP1

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    GFP and RFP array for MbCas12
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBBK31 pHomL-TDH3p-Scarlet(rfp)-Link-GFPdropout-SSA1t-HomR (AmpR)

    Plasmid

    #179015

    Purpose

    Parent vector for markerless yeast integration cassettes with N-terminal Scarlet(rfp)-tagged genes

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pX330_HR_Prnp_3

    Plasmid

    #78621

    Purpose

    pX330 vector encoding SpCas9 and a chimeric guide RNA targeting Prnp coding sequence

    Depositor

    Walker Jackson

    Article

    Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

    Insert

    pX330_HR_Prnp_3
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLsCas13a-gRNA-1

    Plasmid

    #164865

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for LsCas13a in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    LsCas13a-gRNA-1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAN-PBAD-sgRNA-A2T

    Plasmid

    #62258

    Purpose

    expression of A2T sgRNA from the arabinose-inducible promoter

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    A2T sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEPOR0SP0007

    Plasmid

    #117534

    Purpose

    Level0 Golden Gate part: CDS, LbCas12a

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    LbCas12a (with stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAN-PBAD-sgRNA-A1T

    Plasmid

    #62256

    Purpose

    expression of A1T sgRNA from the arabinose-inducible promoter

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    A1T sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge