We narrowed to 4,720 results for: donor

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SGP11_Halo_N_allele

Plasmid

#178171

Purpose

Donor vector for endogenous tagging of human SGP11 at the N-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (SPG11 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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ITM2B_Halo_C_allele

Plasmid

#178160

Purpose

Donor vector for endogenous tagging of human ITM2B at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (ITM2B Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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GCH1_Halo_N_allele

Plasmid

#178159

Purpose

Donor vector for endogenous tagging of human GCH1 at the N-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (GCH1 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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KCNC3_Halo_N_allele

Plasmid

#178129

Purpose

Donor vector for endogenous tagging of human KCNC3 at the N-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (KCNC3 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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PLEKHG4_Halo_C_allele

Plasmid

#178128

Purpose

Donor vector for endogenous tagging of human PLEKHG4 at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (PLEKHG4 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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COQ2_Halo_N_allele

Plasmid

#178154

Purpose

Donor vector for endogenous tagging of human COQ2 at the N-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (COQ2 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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TIA1_Halo_N_allele

Plasmid

#178149

Purpose

Donor vector for endogenous tagging of human TIA1 at the N-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (TIA1 Human)

Use

Donor vector

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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DAO_Halo_C_allele

Plasmid

#178155

Purpose

Donor vector for endogenous tagging of human DAO at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (DAO Human)

Use

Donor vector

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-T2A-Gal80-3xP3-RFP

Plasmid

#127562

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal80-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

Gal80

Use

CRISPR

Expression

Insect

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-T2A-Gal4DBD-3xP3-RFP

Plasmid

#127564

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4DBD-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

Gal4DBD

Use

CRISPR

Expression

Insect

Available Since

July 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-T2A-QF2-3xP3-RFP

Plasmid

#127559

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-QF2-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

QF2

Use

CRISPR

Expression

Insect

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pIW1

Plasmid

#52530

Purpose

serves as PCR-template to generate HR donors for C-terminal Twin-Strep tag

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

Twin-STREP-tag

Use

Blunt cloning kit

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

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pDOC-K-glmS-lacIZ

Plasmid

#158061

Purpose

Donor plasmid for insertion of the lacIZ operon and lac promoter into the S. Typhimurium chromosome

Depositor

Mark Webber

Article

Holden et al Microbiology (Reading). 2020 Nov 23. doi

Insert

lacIZ operon

Use

Synthetic Biology

Expression

Bacterial

Promoter

lac

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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R777-E285 Hs.ROCK2

Plasmid

#70569

Purpose

Gateway ORF clone of human ROCK2 [NM_004850.3] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

ROCK2 (ROCK2 Human)

Use

Gateway Cloning

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-T2A-FLPo-3xP3-RFP

Plasmid

#127561

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-FLPo-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

FLPo

Use

CRISPR

Expression

Insect

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pRB20

Plasmid

#52559

Purpose

serves as PCR-template to generate HR donors for C-terminal YFP-tag

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

eYFP and Blasticigin resistance cassette

Use

Blunt cloning kit

Available Since

April 23, 2014

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-T2A-p65-3xP3-RFP

Plasmid

#127565

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-p65-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

p65

Use

CRISPR

Expression

Insect

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pJRT9

Plasmid

#68386

Purpose

PHA metabolic engineering

Depositor

Trevor Charles

Type

Empty backbone

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pAP1638

Plasmid

#137089

Purpose

Plasmid for PCR-generated donor DNA for rapid tagging of proteins with 4*(GFP11)

Depositor

Geraldine Seydoux

Article

Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

Insert

4*(GFP11)

Use

Pcr template for donor dna

Expression

Bacterial

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAP1836

Plasmid

#137087

Purpose

Plasmid for PCR-generated donor DNA for rapid tagging of proteins with mCherry

Depositor

Geraldine Seydoux

Article

Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

Insert

mCherry

Use

Pcr template for donor dna

Expression

Bacterial

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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