165,191 results

1. psPAX2-D64V

   Plasmid

   #63586

   Purpose

   psPAX2-D64V is derived from psPAX2 plasmid. It is a packaging plasmid for making Integrase deficient lentiviral vectors since has a point mutation in the integrase gene.

   Depositor

   David Rawlings, Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   Packaging plasmid for integrase deficient lentiviral vectors
   

   Use

   Integrase deficient lentiviral packaging vector

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-CMV-jGCaMP8m

   Plasmid

   #162372

   Purpose

   Mammalian expression of ultrafast protein calcium sensor

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Insert

   jGCaMP8m
   

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   A25G F286Y

   Promoter

   CMV

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiSAMv2

   Plasmid

   #75112

   Purpose

   lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 and EF1A

   Available Since

   May 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNL [NlucP/ISRE/Hygro] Vector

   Plasmid

   #236834

   Purpose

   Express ISRE response element in luciferase reporter assay

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   ISRE
   

   Use

   Luciferase

   Tags

   NanoLuc (R)

   Mutation

   None

   Promoter

   minP

   Available Since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pcDNA3.1-2xFLAG-2xSTREP (empty vector)

   Plasmid

   #172604

   Purpose

   Empty vector for the cloning of an N-terminal 2xFLAG-2xSTREP-tagged gene of interest, and expression in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Type

   Empty backbone

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. SB100X in pCAG globin pA

   Plasmid

   #127909

   Purpose

   Sleeping Beauty Transposase expression vector driven by the CAG promoter

   Depositor

   Mark Groudine

   Article

   Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

   Insert

   Sleeping Beauty Transposase
   

   Expression

   Mammalian

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-GFP-LC3-RFP-LC3ΔG

   Plasmid

   #168997

   Purpose

   Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells and zebrafish to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Tags

   EGFP and mRFP1

   Expression

   Mammalian

   Available Since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. dCas9-KRAB-MeCP2

   Plasmid

   #110821

   Purpose

   Improved dCas9 repressor-dCas9-KRAB-MeCP2

   Depositor

   Alejandro Chavez, George Church

   Article

   Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

   Insert

   dCas9-KRAB-MeCP2
   

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CMV

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXPR_120

   Plasmid

   #96917

   Purpose

   for CRISPRa, lentiviral expression of dCas9-VPR 2A BlastR

   Depositor

   David Root, John Doench

   Article

   Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

   Insert

   dCas9
   

   Use

   Lentiviral

   Tags

   VPR

   Expression

   Mammalian

   Mutation

   D10A, N863A

   Promoter

   EF1a

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCardinal-N1

    Plasmid

    #54590

    Purpose

    Localization: N1 Cloning Vector, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Type

    Empty backbone

    Tags

    mCardinal

    Expression

    Mammalian

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pCRIS-PITChv2-BSD-dTAG (BRD4)

    Plasmid

    #91792

    Purpose

    PITCh dTAG donor vector for BSD-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

    Depositor

    James Bradner, Behnam Nabet

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Insert

    BSD-P2A-2xHA-FKBP_F36V
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    Promoterless

    Available Since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. iSpyMac

    Plasmid

    #155014

    Purpose

    Expresses the codon-optimized, engineered iSpyMac Cas9 nuclease in mammalian cells

    Depositor

    Joseph Jacobson

    Article

    Chatterjee et al Nat Commun. 2020 May 18;11(1):2474. doi:

    Insert

    iSpyMac
    

    Expression

    Mammalian

    Mutation

    PID grafted onto SpyCas9 N-terminal domain, R221K…

    Promoter

    EF1ɑ

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYD1

    Plasmid

    #73447

    Purpose

    expression vector designed for expression, secretion, and display of proteins on the extracellular surface of Saccharomyces cerevisiae cells

    Depositor

    Dane Wittrup

    Article

    Kieke et al Protein Eng. 1997 Nov;10(11):1303-10.

    Type

    Empty backbone

    Tags

    6xHis, AGA2, T7 peptide, V5 tag, and Xpress tag

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. PARP1 FL

    Plasmid

    #169815

    Purpose

    Expresses codon optimised full length His tagged PARP1 in bacterial cells

    Depositor

    David Neuhaus

    Article

    Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226

    Insert

    Poly[ADP-ribose] polymerase 1 (PARP1 Human, Synthetic, Codon optimised)
    

    Tags

    MKHHHHHHMKQ

    Expression

    Bacterial

    Mutation

    Valine 762 mutated to an alanine

    Promoter

    T7 promoter

    Available Since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET15b-AavLEA1

    Plasmid

    #53093

    Purpose

    AavLEA1 expression (in BL21 DE3 E. coli strain)

    Depositor

    Claude Férec

    Article

    Tripathi et al PLoS One. 2014 Aug 13;9(8):e104970. doi:

    Insert

    AavLEA1
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCA24N-ligase

    Plasmid

    #87741

    Purpose

    IPTG-inducible expression of T4 DNA ligase for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T5-lac

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. minCMV-1xmNG-dMCP

    Plasmid

    #231091

    Purpose

    Encodes a 1xmNeonGreen-dMCP under control of a weak promoter (minCMV).

    Depositor

    John Ngo

    Article

    Kuffner et al Nat Methods. 2025 Sep 22. doi: 10.1038/s

    Insert

    1xmNG-dMCP
    

    Expression

    Mammalian

    Available Since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti6.2_mNeonGreen2

    Plasmid

    #113727

    Purpose

    Expresses the fluorescent protein mNeonGreen2 in a lentiviral backbone

    Depositor

    Vanessa LaPointe

    Article

    Wieland et al Front Bioeng Biotechnol. 2021 Sep 9;9:72

    Insert

    mNeonGreen2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Anti-PSD-95 [K28/43R]

    Plasmid

    #128618

    Purpose

    Mammalian Expression Plasmid of anti-PSD-95 (Human). Derived from hybridoma K28/43.

    Depositor

    James Trimmer

    Article

    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    Anti-PSD-95 (Human) recombinant mouse monoclonal antibody (DLG4 Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCasSA

    Plasmid

    #98211

    Purpose

    CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9

    Depositor

    Quanjiang Ji

    Article

    Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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