166,712 results

1. PINK1 gRNA (BRDN0001147554)

   Plasmid

   #78035

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PINK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PINK1 (PINK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   June 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiMPH v2

   Plasmid

   #89308

   Purpose

   lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.

   Depositor

   Feng Zhang

   Article

   Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

   Insert

   MS2-P65-HSF1_2A_Hygro (RELA Synthetic, Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   N55K in MS2

   Promoter

   EF1a

   Available Since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-AncMEcoGII291

   Plasmid

   #205024

   Purpose

   Expressing engineered M.EcoGII with higher thermostability and DNA methylation activity

   Depositor

   Paola Laurino

   Article

   Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

   Insert

   Ancestral M.EcoGII 291
   

   Tags

   His6x tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAVU6+27-F30-2xdBroccoli

   Plasmid

   #66842

   Purpose

   Expresses two dimeric Broccoli aptamers in the F30 scaffold in mammalian cells

   Depositor

   Samie Jaffrey

   Article

   Filonov et al Chem Biol. 2015 May 21;22(5):649-60. doi

   Insert

   Two dimeric Broccoli aptamers in the F30 scaffold
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. cfSGFP2-col4a1

   Plasmid

   #160317

   Purpose

   cfSGFP2-tagged collagen4a1 for mammalian expression

   Depositor

   Ikuo Wada

   Article

   Matsui et al Cell Struct Funct. 2020 Jul 23;45(2):107

   Insert

   COL4A1 (COL4A1 Human)
   

   Tags

   cfSGFP2

   Expression

   Mammalian

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYFP-RPB1aAmr

   Plasmid

   #75284

   Purpose

   Expresses alpha-amanitin resistant EYFP tagged POLR2A in mammalian cells

   Depositor

   Xavier Darzacq, Robert Singer

   Article

   Darzacq et al Nat Struct Mol Biol. 2007 Sep;14(9):796-

   Insert

   POLR2A (POLR2A Human)
   

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   N792D alpha-amanitin–resistant

   Promoter

   CMV

   Available Since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-3'3xFlag

   Plasmid

   #208616

   Purpose

   Empty vector for mammalian expression, insert 3xFlag tag using BamHI andAflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with 3xFlag

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Orai1 CFP

   Plasmid

   #19757

   Depositor

   Anjana Rao

   Article

   Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

   Insert

   Orai1 (ORAI1 Human)
   

   Tags

   CFP

   Expression

   Mammalian

   Available Since

   Nov. 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti6.3-sRGN3.1-BlastR

   Plasmid

   #220964

   Purpose

   Expresses human codon-optimized sRGN3.1

   Depositor

   Hyongbum Kim

   Article

   Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

   Insert

   sRGN3.1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cas12f-GE ver4.1

    Plasmid

    #176544

    Purpose

    Expresses Cas12f-GE in mammalian cells

    Depositor

    Yong-Sam Kim

    Article

    Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

    Inserts

    Cas12f-GE ver4.1
    Cas12f-GE ver4.1
    

    Expression

    Mammalian

    Promoter

    U6 and chicken β-actin

    Available Since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-Neo-Bam p53 wt

    Plasmid

    #16434

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Insert

    p53 (TP53 Human)
    

    Expression

    Mammalian

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-Cyto-iPASnFR-L-mScarlet3-3xFLAG

    Plasmid

    #225242

    Purpose

    Mammalian expression of iPASnFR-L in the cytosol

    Depositor

    Loren Looger

    Article

    Tamura et al bioRxiv 2024.08.21.609037

    Insert

    iPASnFR-L
    

    Tags

    3xFLAG and mScarlet3

    Expression

    Mammalian

    Available Since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAS

    Plasmid

    #60847

    Purpose

    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast

    Depositor

    Jamie Cate

    Article

    Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife

    Inserts

    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    NLS/His8 and TTT 3' extension prior to sgRNA

    Expression

    Bacterial and Yeast

    Mutation

    L4 is UUCG tetraloop and guide targets LYP1 (CATA…

    Available Since

    Jan. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJT126 lenti pEF-rTetR(SE-G72P)-3XFLAG-LibCloneSite-T2A-mCherry-BSD-WPRE

    Plasmid

    #161926

    Purpose

    Lentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

    Insert

    rTetR(SE-G72P)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    pEF

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNIC28-DENV3-NS5FL

    Plasmid

    #86013

    Purpose

    Expression of dengue virus 3 NS5 protein

    Depositor

    Dahai Luo

    Article

    Zhao et al PLoS Pathog. 2015 Mar 16;11(3):e1004682.

    Insert

    DENV3-NS5FL
    

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    amino acid residues 6 to 895 of DENV3 NS5

    Promoter

    T7

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti.CAG.H2B-Dendra2.W

    Plasmid

    #51005

    Purpose

    Lentivector that expresses H2B-Dendra2 from internal CAG enhancer-promoter

    Depositor

    Rusty Lansford

    Insert

    H2B-Dendra2
    

    Use

    Lentiviral

    Tags

    histone2B

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-Pur-mEGFP

    Plasmid

    #186329

    Purpose

    Fluorescent reporter system

    Depositor

    Bertrand Collet

    Insert

    mEGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-Cyto-iPASnFR-H-mScarlet3-3xFLAG

    Plasmid

    #225241

    Purpose

    Mammalian expression of iPASnFR-H in the cytosol

    Depositor

    Loren Looger

    Article

    Tamura et al bioRxiv 2024.08.21.609037

    Insert

    iPASnFR-H
    

    Tags

    3xFLAG and mScarlet3

    Expression

    Mammalian

    Available Since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDisplay-GRAB_r5-HT1.0

    Plasmid

    #208713

    Purpose

    Expresses the red 5-HT sensor GRAB_r5-HT1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

    Insert

    Red fluorescent 5-HT sensor GRAB_r5-HT1.0
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJR85

    Plasmid

    #140095

    Purpose

    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.

    Depositor

    Jonathan Weissman

    Article

    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

    Insert

    sgRNA with cs1 in stem loop 2 of the constant region
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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