162,717 results

1. Lys-eMagB-iRFP

   Plasmid

   #162249

   Purpose

   Blue-light dependent heterodimerization system

   Depositor

   Pietro De Camilli

   Article

   Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

   Insert

   p18-eMagB-iRFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   beta-actin promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF0978

   Plasmid

   #141775

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   KLF7 (KLF7 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mCherry-N2-Frankenbody-HA

   Plasmid

   #175292

   Purpose

   Visualize endogeneous loci coupled with ZF probes harboring 10xHA tag

   Depositor

   Tim Stasevich

   Article

   Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

   Insert

   anti-HA frankenbody fused with mCherry
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET15TV-L-AldA WT

   Plasmid

   #187976

   Purpose

   Expresses human aldolase A

   Depositor

   Audrey Lamb

   Article

   Fenton et al Protein Sci. 2022 Feb;31(2):357-370. doi

   Insert

   AldA WT (ALDOA Human)
   

   Use

   Tags

   Hexahistag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. eMagB-iRFP-Mito

   Plasmid

   #162250

   Purpose

   Blue-light dependent heterodimerization system

   Depositor

   Pietro De Camilli

   Article

   Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

   Insert

   eMagB-iRFP-OMP25
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   beta-actin promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MSCV-N SV40 LT

   Plasmid

   #37857

   Depositor

   Karl Munger

   Article

   Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

   Insert

   SV40 LT (SV40gp6 Simian Virus 40)
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   PKG

   Available since

   July 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV-Dre-T2A-TagRFP

   Plasmid

   #167968

   Purpose

   Transient expression of Dre and TagRFP

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   Dre recombinase
   

   Use

   Tags

   TagRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pWPXL-c-Myc

   Plasmid

   #36980

   Depositor

   Bob Weinberg

   Article

   Guo et al Cell. 2012 Mar 2;148(5):1015-28.

   Insert

   c-Myc (Myc Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pHR_SFFv_4D5-Low-CAR_RHL001

   Plasmid

   #164824

   Purpose

   Lentiviral vector for constitutive expression of AntiHER2 4D5-Low CAR

   Depositor

   Wendell Lim

   Article

   Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

   Insert

   AntiHER2 4D5-Low Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3_LuxSit_i_mTagBFP2

    Plasmid

    #194300

    Purpose

    Mammalian expression of the de novo luciferase, LuxSit-i

    Depositor

    Andy Yeh, David Baker

    Article

    Yeh et al Nature. 2023 Feb;614(7949):774-780. doi:

    Insert

    LuxSit-i
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHAGE2 mCherry-Atl1

    Plasmid

    #86678

    Purpose

    Lentivirus to stably express fluorescent protein in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    ATL1 (ATL1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. 2HA-pDEST-N

    Plasmid

    #118373

    Purpose

    Gateway compatible mammalian expression vector with N-terminal 2HA to tag proteins of interest.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    2HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFP-hCaM

    Plasmid

    #47602

    Depositor

    Emanuel Strehler

    Insert

    CaM (CALM3 Human)
    

    Use

    Tags

    EGFP-HA-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TFORF2823

    Plasmid

    #143629

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    IKZF1 (IKZF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA-dCas9

    Plasmid

    #47106

    Purpose

    Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Flag and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive)

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIRES-H3.3-WT-FLAG-BFP

    Plasmid

    #206139

    Purpose

    Expression of a FLAG tagged human histone H3.3 gene with BFP

    Depositor

    Maria Castro

    Insert

    H3.3 (H3-3A Human)
    

    Use

    Tags

    FLAG-eBFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJ204-loxP-EF1a-tagBFP-HygR-lox2272-BGHpA-siteT9-HDR

    Plasmid

    #154827

    Purpose

    Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site T9)

    Depositor

    Lise Marie Grav

    Article

    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

    Inserts

    TagBFP
    HygR
    ZsGreen1-DR
    

    Use

    CRISPR and Cre/Lox

    Tags

    PEST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, EF1a, and SV40 early promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. eMagBF-TgRFPT

    Plasmid

    #162253

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    eMagBF-TgRFPT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-GFAP-hChR2(H134R)-EYFP

    Plasmid

    #27054

    Purpose

    AAV expression of humanized ChR2(H134R) fused to EYFP, for optogenetic excitation

    Depositor

    Karl Deisseroth

    Insert

    hChR2(H134R)
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    GFAP

    Available since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-ReaChR-citrine

    Plasmid

    #50956

    Purpose

    ReaChR-citrine in lentival transfer vector under human synapsin promoter

    Depositor

    Roger Tsien

    Article

    Lin et al Nat Neurosci. 2013 Oct;16(10):1499-508.

    Insert

    ReaChR-citrine
    

    Use

    Lentiviral

    Tags

    citrine

    Expression

    Mutation

    Promoter

    human synapsin promoter

    Available since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge