165,187 results

1. pIN1 (gpdA-cTerm-mGL-ptrA)

   Plasmid

   #188735

   Purpose

   Expresses mGreenlantern for C-terminal tagging

   Depositor

   Michael Bromley

   Article

   Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

   Insert

   gdpA-mGreenlantern PtrA
   

   Use

   Filamentous fungal expression

   Tags

   mGreenlantern

   Promoter

   gpdA

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIN2 (gpdA-nTerm-mGL-ptrA)

   Plasmid

   #188736

   Purpose

   Expresses mGreenlantern for N-terminal tagging

   Depositor

   Michael Bromley

   Article

   Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

   Insert

   gdpA-mGreenlantern PtrA
   

   Use

   Filamentous fungal expression

   Tags

   mGreenlantern

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. dCas9 fused to scFv (anti-spike)

   Plasmid

   #186421

   Purpose

   Expression of dCas9 with C-terminal nanobody fusion recognizing spike protein from SARS-CoV-2

   Depositor

   Stephen Elledge

   Article

   Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

   Insert

   dCas9-scFv fusion (anti-SARS-CoV-2 spike)
   

   Use

   CRISPR

   Tags

   6His

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNIC28-SENP1

   Plasmid

   #196354

   Purpose

   protein expression of the His-tagged catalytic domain

   Depositor

   Stefan Knapp

   Insert

   SENP1 catalytic domain
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   contains only E419-L644

   Promoter

   T7

   Available Since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVITRO1-mCherry-LaminB1

   Plasmid

   #186348

   Purpose

   Nuclear membrane-targeted mCherry expression in mammalian cells

   Depositor

   Chuan-Hsiang Huang

   Article

   Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

   Insert

   mCherry-LaminB1
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Rat EF1α

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTS2307-Tier1-2xcHS4-PPGK-NLuc_TB

   Plasmid

   #169550

   Purpose

   Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a tandem repeat of a 5' cHS4 insulator sequence (2xcHS4-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   cHS4-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
   

   Expression

   Mammalian

   Promoter

   PPGK

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. miniABEMax V82G nSaCas9

   Plasmid

   #135365

   Purpose

   Expression of SaCas9 nickase (D10A) with single evolved TadA monomer (ABEMax) with a V82G substitution

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   miniABEMax V82G nSaCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   (V82G)

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5-F-Med26-NTD R61A K62A

   Plasmid

   #49256

   Purpose

   Mammalian expression of flag-tagged human Med26-NTD R61A K62A

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

   Insert

   Med 26 (MED26 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   NTD R61A K62A

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA5-F-Med26-NTD

   Plasmid

   #49255

   Purpose

   Mammalian expression of flag-tagged Med26-NTD

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

   Insert

   Med 26 (MED26 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   NTD (aa 1-339)

   Available Since

   Dec. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA5-F-Med26-R61A K62A

    Plasmid

    #49254

    Purpose

    Mammalian expression of flag-tagged human Med26 R61A K62A

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

    Insert

    Med26 (MED26 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    R61A K62A

    Available Since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

11. HA-Ubiquitin

    Plasmid

    #18712

    Purpose

    Mammalian expression of human ubiquitin with HA tag

    Depositor

    Edward Yeh

    Article

    Kamitani et al J Biol Chem. 1997 Nov 7. 272(45):28557-6

    Insert

    ubiquitin (UBB Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    The HA-Ubiquitin insert is from the UBB gene. The…

    Available Since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-UbC-rtTA-Ngn2:2A:Ascl1

    Plasmid

    #127289

    Purpose

    all-in-one inducible LV expression construct for Ngn2:2A:Ascl1 (aka UNA; for direct neuronal conversion)

    Depositor

    Fred Gage

    Article

    Herdy et al Elife. 2019 May 17;8. pii: 41356. doi: 1

    Inserts

    Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
    Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
    

    Use

    Lentiviral

    Tags

    Ngn2 and Ascl1 linked via 2A

    Expression

    Mammalian

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmBaoJin-Actin

    Plasmid

    #229609

    Purpose

    Expresses mBaoJin-actin fusion in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

    Insert

    mBaoJin
    

    Expression

    Mammalian

    Available Since

    July 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLCAR-CD19-28z

    Plasmid

    #135991

    Purpose

    Modular CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Scott McComb

    Article

    Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

    Insert

    FMC63-28-3z-P2A-EGFP (CD28, CD247 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a-short

    Available Since

    June 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. CD63-pEGFP C2

    Plasmid

    #62964

    Purpose

    full length human CD63 cloned into pEGFP C2

    Depositor

    Paul Luzio

    Insert

    CD63 molecule (CD63 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CD63 has 1 silent mutation compared to the coding…

    Promoter

    CMV

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. TRUPATH Triple Gai2

    Plasmid

    #196049

    Purpose

    Encodes a G alpha subunit (GNAl2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphai2-RLuc8 (GNAI2 Human)
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. TRUPATH Triple (bidirectional) GasS

    Plasmid

    #196055

    Purpose

    Encodes a G alpha subunit (GNAS2) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphasS-RLuc8
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-NPC1(WT)-FLAG

    Plasmid

    #164972

    Purpose

    Lentiviral vector for NPC1 expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

    Insert

    NPC1 (NPC1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. mEGFP-NbALFA

    Plasmid

    #159986

    Purpose

    For the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Insert

    mEGFP-NbALFA
    

    Tags

    8histidine-HRV3C protease cleavage site

    Expression

    Bacterial

    Mutation

    3GS linker between mEGFP and NbALFA

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTRIPZ shNS

    Plasmid

    #127696

    Purpose

    Doxycyclin inducible pTRIPZ lentivirus vector containing a non-silencing sequence for both human and mouse

    Depositor

    Sandra Demaria

    Article

    Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

    Insert

    shNS
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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