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3xFLAG-dCas9/pCMV-7.1 Citations (12)

Originally described in: Efficient isolation of specific genomic regions and identification of associated proteins by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR.
Fujita T, Fujii H Biochem Biophys Res Commun. 2013 Sep 13;439(1):132-6. doi: 10.1016/j.bbrc.2013.08.013. Epub 2013 Aug 11.
PubMed Journal

Articles Citing 3xFLAG-dCas9/pCMV-7.1

Isolation of specific genomic regions and identification of associated molecules by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR. Fujita T, Fujii H. Methods Mol Biol. 2015;1288:43-52. doi: 10.1007/978-1-4939-2474-5_4. PubMed
Isolation of Specific Genomic Regions and Identification of Their Associated Molecules by Engineered DNA-Binding Molecule-Mediated Chromatin Immunoprecipitation (enChIP) Using the CRISPR System and TAL Proteins. Fujii H, Fujita T. Int J Mol Sci. 2015 Sep 9;16(9):21802-12. doi: 10.3390/ijms160921802. PubMed
Regulation of the cohesin-loading factor NIPBL: Role of the lncRNA NIPBL-AS1 and identification of a distal enhancer element. Zuin J, Casa V, Pozojevic J, Kolovos P, van den Hout MCGN, van Ijcken WFJ, Parenti I, Braunholz D, Baron Y, Watrin E, Kaiser FJ, Wendt KS. PLoS Genet. 2017 Dec 20;13(12):e1007137. doi: 10.1371/journal.pgen.1007137. eCollection 2017 Dec. PubMed
Delete_enChIP systems using different CRISPR orthologues and epitope tags. Fujita T, Yuno M, Fujii H. BMC Res Notes. 2018 Feb 27;11(1):154. doi: 10.1186/s13104-018-3262-4. PubMed
Promoter-associated proteins of EPAS1 identified by enChIP-MS - A putative role of HDX as a negative regulator. Hamidian A, Vaapil M, von Stedingk K, Fujita T, Persson CU, Eriksson P, Veerla S, De Preter K, Speleman F, Fujii H, Pahlman S, Mohlin S. Biochem Biophys Res Commun. 2018 May 5;499(2):291-298. doi: 10.1016/j.bbrc.2018.03.150. Epub 2018 Mar 26. PubMed
Rational targeting of a NuRD subcomplex guided by comprehensive in situ mutagenesis. Sher F, Hossain M, Seruggia D, Schoonenberg VAC, Yao Q, Cifani P, Dassama LMK, Cole MA, Ren C, Vinjamur DS, Macias-Trevino C, Luk K, McGuckin C, Schupp PG, Canver MC, Kurita R, Nakamura Y, Fujiwara Y, Wolfe SA, Pinello L, Maeda T, Kentsis A, Orkin SH, Bauer DE. Nat Genet. 2019 Jul;51(7):1149-1159. doi: 10.1038/s41588-019-0453-4. Epub 2019 Jun 28. PubMed
Interrogation of enhancer function by enhancer-targeting CRISPR epigenetic editing. Li K, Liu Y, Cao H, Zhang Y, Gu Z, Liu X, Yu A, Kaphle P, Dickerson KE, Ni M, Xu J. Nat Commun. 2020 Jan 24;11(1):485. doi: 10.1038/s41467-020-14362-5. PubMed

Associated Plasmids

Coxiella effector protein CvpF subverts RAB26-dependent autophagy to promote vacuole biogenesis and virulence. Siadous FA, Cantet F, Van Schaik E, Burette M, Allombert J, Lakhani A, Bonaventure B, Goujon C, Samuel J, Bonazzi M, Martinez E. Autophagy. 2021 Mar;17(3):706-722. doi: 10.1080/15548627.2020.1728098. Epub 2020 Mar 1. PubMed
Alu retrotransposons modulate Nanog expression through dynamic changes in regional chromatin conformation via aryl hydrocarbon receptor. Gonzalez-Rico FJ, Vicente-Garcia C, Fernandez A, Munoz-Santos D, Montoliu L, Morales-Hernandez A, Merino JM, Roman AC, Fernandez-Salguero PM. Epigenetics Chromatin. 2020 Mar 14;13(1):15. doi: 10.1186/s13072-020-00336-w. PubMed
Mass spectrometry-based proteomic capture of proteins bound to the MACC1 promoter in colon cancer. Huang Y, Xiang Y, Xie Z, Cai Y, Yang Q, Huang H, Chen Z, Xiao Z, He Q. Clin Exp Metastasis. 2020 Aug;37(4):477-487. doi: 10.1007/s10585-020-10045-z. Epub 2020 Jul 1. PubMed
Dynamic and widespread control of poly(A) tail length during macrophage activation. Kwak Y, Daly CWP, Fogarty EA, Grimson A, Kwak H. RNA. 2022 Jul;28(7):947-971. doi: 10.1261/rna.078918.121. Epub 2022 May 5. PubMed
A polycistronic transgene design for combinatorial genetic perturbations from a single transcript in Drosophila. Teague AG, Quintero M, Karimi Dermani F, Cagan RL, Bangi E. PLoS Genet. 2023 Jun 2;19(6):e1010792. doi: 10.1371/journal.pgen.1010792. eCollection 2023 Jun. PubMed

Associated Plasmids

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