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Plasmid#113082PurposeEnhanced RNAi vektor for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites and a lacZ coding sequence.DepositorTypeEmpty backboneUseRNAiTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only
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pET28a-AncMEcoGII291
Plasmid#205024PurposeExpressing engineered M.EcoGII with higher thermostability and DNA methylation activityDepositorInsertAncestral M.EcoGII 291
UseTagsHis6x tagExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-2A-eGFP(W58X) pcDNA3.1(-)
Plasmid#172229PurposeExpresses an mCherry-2A-eGFP dual reporter that contains a UAG codon in position 58 (W58X) of eGFP, in mammalian cellsDepositorInsertmCherry-2A-eGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
LRTi
Plasmid#214072PurposeLuminescent reporter system to measure EJC-enhanced NMD activity. No intron in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationLinker sequence present between firefly luciferas…PromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMUH_unc84_2XGFP
Plasmid#46023DepositorInsertUNC-84 (unc-84 )
Use10xuas-phi31-expression vectorTags2XGFPExpressionMutationTwo amino acid mutations (F279L and L500P) compar…Promoter10XUASAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDae029 (eMutaT7[TadA-8e])
Plasmid#187620PurposeExpresses eMutaT7[TadA-8e] for mutagenizing genes downstream from a T7 promoter in e.coliDepositorInsertTadA-8e-T7RNAP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiRT_RTctrl
Plasmid#214070PurposeFluorescent reporter system, readthrough control, NMD negative control. Intron present in 5'UTRDepositorInsertTurboRFP
UseTagsExpressionMammalianMutationLinker sequence present between RFP and GFPPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AncMEcoGII250
Plasmid#205025PurposeExpressing engineered M.EcoGII with higher thermostabilityDepositorInsertAncestral M.EcoGII 250
UseTagsHis6x tagExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
LRTX_ctrl
Plasmid#214073PurposeLuminescent reporter system, NMD negative control. No intron in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationRemnant of linker sequence present at the end of …PromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYZ463
Plasmid#187971PurposeTEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4tDepositorInsertCas9 (codon optimized for human)
UseSynthetic BiologyTagsSV40ExpressionYeastMutationPromoterTEF1Available sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSEVA63_Helper
Plasmid#190432PurposeM13 helper genes cloned into the pSEVA631 vector. This plasmid does not carry an M13 packaging signal. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ297.)DepositorInsertM13 genes: I-XI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSB1K3_M13ps_LacZα_gIII
Plasmid#190435PurposeM13 packaging signal, LacZα gene, and M13 gIII gene (also called gp3, or g3p) cloned into the pSB1K3 vector.DepositorInsertsM13 packaging signal
M13 gIII
LacZα
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYZ462
Plasmid#187970PurposeTEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4tDepositorInsertCas9 (codon optimized for human)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterTEF1Available sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBTK622
Plasmid#180592Purposetdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1DepositorInsertstdk
kanR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
ptRNAfMet-A37
Plasmid#196657PurposePlasmid for overexpression of native initiator tRNA (A37)DepositorInserttRNAfMet-A37 expression cassette
UseTagsExpressionBacterialMutationPromotermetY native promoterAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
LRT_RTctrl
Plasmid#214074PurposeLuminescent reporter system, readthrough control, NMD negative control. No intron in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationLinker sequence present between firefly luciferas…PromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
LRT
Plasmid#214071PurposeLuminescent reporter system to measure EJC-independent NMD activity. No intron in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationLinker sequence present between firefly luciferas…PromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD_Kan-LacI
Plasmid#79826PurposeArabinose induced expression of Lac repressorDepositorInsertLacI
UseTagsHis6ExpressionBacterialMutationPromoteraraBpAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYZ038
Plasmid#200227Purposeuniversal template to build tRNA-gRNA array for multiplex editing CREEPYDepositorInserttRNA-gRNA scaffold
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmax-3xFLAG_3xNLS_dPspCas13b(AAAA)_2xNLS-Clover
Plasmid#196829PurposeExpress dpdpCas13b with AAAA mutation and CloverDepositorInsertdpspCas13b(AAAA)
UseTags3XFLAG and NESExpressionMammalianMutationK367A, D369A and K370APromoterAvailable sinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only