We narrowed to 28,549 results for: PLE;

1. pLenti-Synapsin-pHoenix-WPRE

   Plasmid

   #70111

   Purpose

   lentiviral expression of pHoenix (Synaptophysin-pHluorin-Arch3-mKate2) driven by human Synapsin I promoter for optogenetic acidification of synaptic vesicles

   Depositor

   Christian Rosenmund

   Article

   Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

   Insert

   pHoenix (Syp Rat, Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria)
   

   Use

   Lentiviral

   Tags

   Arch3, H+/K+ ATPase beta-subunit, mKate2, and pHl…

   Expression

   Mutation

   Promoter

   Synapsin

   Available since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLenti-FNLS-P2A-Puro

   Plasmid

   #110841

   Purpose

   Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA-FNLS
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D10A and NLS sequence at the N-terminus

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti-EGFP-P4M-SidMx2

   Plasmid

   #136997

   Purpose

   Lentiviral EGFP lipid sensor for PI(4)P

   Depositor

   Ken-Ichi Takemaru

   Insert

   EGFP-P4M-SidMx2
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   EF1 alpha

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLentiPGK Puro DEST ERKKTRClover

   Plasmid

   #90227

   Purpose

   Lentiviral vector to express ERK KTR mClover under PGK promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   ERK Kinase Translocation Reporter
   

   Use

   Lentiviral

   Tags

   mClover

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLEX-MCS PGK promoter

   Plasmid

   #128044

   Purpose

   Lentiviral vector with PGK promoter

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti Lifeact-EGFP BlastR

   Plasmid

   #84383

   Purpose

   Lentiviral expression of EGFP-tagged Lifeact with Blasticidin selection in cells including neurons

   Depositor

   Ghassan Mouneimne

   Article

   Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

   Insert

   Lifeact
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   CMV immediate early

   Available since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLentiPGK Hygro DEST ERKKTRmCerulean3

   Plasmid

   #90229

   Purpose

   Lentiviral vector to express ERK KTR mCerulean3 under PGK promoter (With Hygromycin Resistance)

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   ERK Kinase Translocation Reporter
   

   Use

   Lentiviral

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti-CD74-ROS1 nonmutant

   Plasmid

   #183813

   Purpose

   Express CD74-ROS1 fusion (C6;R34) in mammalian cells

   Depositor

   Aaron Hata

   Article

   Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

   Inserts

   ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
   CD74 molecule (CD74 Human)
   

   Use

   Lentiviral

   Tags

   The fusion protein of CD74 exon 1-6 and ROS1 exon…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Plasmid L5 attB::Pleft* mScarlet

   Plasmid

   #169410

   Purpose

   Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mScarlet

   Depositor

   Clifton Barry III

   Article

   Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

   Inserts

   pLeft
   mScarlet proper
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-Tet-MKK6(DD)-Puro

    Plasmid

    #86094

    Purpose

    Tet/Dox inducible (TetR) constitutively active mutant MKK6/MAP2K6 in lentiviral vector

    Depositor

    Cindy Miranti

    Article

    Frank et al J Cell Sci. 2017 Apr 26. pii: jcs.197152

    Insert

    MKK6 (MAP2K6 Human)
    

    Use

    Lentiviral

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Serine 207 and Threonine 211 both changed to Aspa…

    Promoter

    CMV/TetO

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLentiPGK Blast DEST JNKKTRmRuby2

    Plasmid

    #59154

    Purpose

    Lentiviral vector to express JNK KTR mRuby2 under PGK promoter (With Blasticidin Resistance)

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    JNK Kinase Translocation Reporter (MAPK8 Human)
    

    Use

    Lentiviral

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Sept. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CLYBL-TO-hNIL-BSD-mApple

    Plasmid

    #124230

    Purpose

    Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2-ISL1-LHX3 for differentiation of iPSCs into lower motor neurons. Contains a floxed BSD-NLS-mApple selection cassette.

    Depositor

    Michael Ward

    Insert

    NGN2, ISL1, LHX3 (NEUROG2, ISL1, LHX3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti X1 Puro DEST (694-6)

    Plasmid

    #17297

    Purpose

    3rd gen lentiviral promoter-less Gateway destination vector, Puro

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi; Destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PB-TO-NGN2-EMX1 BSD-mApple

    Plasmid

    #182311

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 and EMX1 expression, bsd selection, mApple

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Inserts

    hNGN2-P2A-EMX1 (NEUROG2, EMX1 Human)
    bsd-T2A-mycNLS-mApple
    

    Use

    Tags

    T2A-mycNLS-mApple

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and TRE3G

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-dCas9-T2A-GFP

    Plasmid

    #193781

    Purpose

    Tricistronic expression of dCas9-EGFP-BSD

    Depositor

    James Eshleman

    Article

    Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

    Insert

    dead Cas9
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    In frame with existing Cas9 promoter

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-PH-PLC-delta-EGFP

    Plasmid

    #136998

    Purpose

    Lentiviral EGFP lipid sensor for PI(4,5)P2

    Depositor

    Ken-Ichi Takemaru

    Insert

    PH-PLC-delta-EGFP
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    EF1 alpha

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLentiPGK Puro DEST p38KTRClover

    Plasmid

    #59152

    Purpose

    Lentiviral vector to express p38 KTR mClover under PGK promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Sept. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti SpBsmBI sgRNA Hygro

    Plasmid

    #62205

    Purpose

    An empty sgRNA expression vector for expression of sgRNA for Sp Cas9

    Depositor

    Rene Maehr

    Article

    Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLEX-EF1a ANXA11-mEmerald

    Plasmid

    #164210

    Purpose

    pLEX lentivirus backbone expresses mEmerald tagged ANXA11 under EF1a promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti- V6.3 Ultra-Chili

    Plasmid

    #106173

    Purpose

    lentiviral CMV driven tdTomato-P2A-T2A, Blasticidin selectable

    Depositor

    Ewa Snaar-Jagalska

    Insert

    dTomato
    

    Use

    Lentiviral

    Tags

    T2A, P2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge