Addgene

We narrowed to 4,184 results for: Pet2

Showing: 1241 - 1260 of 4184 results

Results per page:

Sort results by:

pET21-pelB-LaTdT-9

Plasmid

#228977

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-9, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-9

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaTdT-39

Plasmid

#228980

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-39

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-SUMO-SeKaiA

Plasmid

#229218

Purpose

For expression of S. elongatus KaiA protein

Depositor

Andy LiWang

Insert

kaiA (kaiA S. elongatus)

Use

Tags

FLAG and SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET20b-mPTER-R204A

Plasmid

#222196

Purpose

Bacterial expression of mouse PTER-R204A protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

R204A

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-acatenin.22-906

Plasmid

#186460

Purpose

Expresses human alpha-catenin (residues 22-906) with an N-terminal His tag and a PRescission Protease cleavage site

Depositor

Tina Izard

Article

Rangarajan et al Commun Biol. 2023 Mar 16;6(1):276. doi:

Insert

CTNNA1 (CTNNA1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-CsHPAD_beta-gamma

Plasmid

#226981

Purpose

Expressing Clostridium scatologenes hydroxyphenylacetate decarboxylase (HPAD) beta/gamma subunits in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

CsHPAD_beta-gamma

Use

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-OuIAD(W392A)

Plasmid

#226980

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) W392A variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(W392A)

Use

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

W392A

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-OuIAD(W392F)

Plasmid

#226979

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) W392F variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(W392F)

Use

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

W392F

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-OuIAD(F401A)

Plasmid

#226978

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) F401A variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(F401A)

Use

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

F401A

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-OuIAD(L616E)

Plasmid

#226977

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) L616E variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(L616E)

Use

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

L616E

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-OuIAD(R226K)

Plasmid

#226976

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) R226K variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(R226K)

Use

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

R226K

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-OuIAD(R226M)

Plasmid

#226975

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) R226M variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(R226M)

Use

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

R226M

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-OuIAD(R226E)

Plasmid

#226974

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) R226E variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(R226E)

Use

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

R226E

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-OuIAD(H514E)

Plasmid

#226972

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) H514E variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(H514E)

Use

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

H514E

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-OuIAD(H514A)

Plasmid

#226971

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) H514A variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(H514A)

Use

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

H514A

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-OuIAD(C500S)

Plasmid

#226970

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) C500S variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(C500S)

Use

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

C500S

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-OuIAD(G853A)

Plasmid

#226969

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) G853A variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(G853A)

Use

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

G853A

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a(+)-NEDD4L_W4-HECT

Plasmid

#187710

Purpose

Express his-tagged NEDD4L W4-HECT in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 543-975 (NEDD4L Human)

Use

Tags

His-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-542

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a(+)-NEDD4L_W3-W4-HECT_C874only

Plasmid

#187711

Purpose

Express his-tagged NEDD4L W3-W4-HECT_C874only in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 492-975 with mutations (NEDD4L Human)

Use

Tags

His-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-491; mutations C702A, C776…

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28:LanIV-Streptomyces

Plasmid

#226250

Purpose

Expresses precursor peptide and lanthipeptide synthetase from Streptomyces LanIV (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanIV-Streptomyces)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 1241 - 1260 of 4184 results

Results per page:

Sort results by: