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pET-SUMO mCER-Prx2

Plasmid

#158226

Purpose

Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (Recombinant bacterial expression)

Depositor

Tobias Dick

Publication

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

mCER-Prx2

Expression

Bacterial

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a - CPX

Plasmid

#89620

Purpose

[Edit] Expresses recombinant 6HIS tagged T. cruzi cytoplasmic tryparedoxin peroxidase protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Publication

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Cytosolic Tryparedoxin peroxidase

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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PCW57.1-ER-GshF-GFP

Plasmid

#257607

Purpose

Doxycycline-inducible expression of ER-targeted GshF-GFP

Depositor

Kivanc Birsoy

Publication

Liu et al Nat Cell Biol. 2026 Apr 17. doi: 10.1038

Insert

ER-GshF

Expression

Mammalian

Promoter

tight TRE promoter

Available since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pLRD26

Plasmid

#208273

Purpose

Production of levopimaradiene in E. coli MEV20

Depositor

Christopher Voigt

Publication

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
LPS

Expression

Bacterial

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLRD10

Plasmid

#208262

Purpose

Production of labda-7,13(16),14-triene in E. coli MEV20

Depositor

Christopher Voigt

Publication

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

grTPS2
sclav_p0491

Expression

Bacterial

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGWB602-TP-mCherry-UnaG

Plasmid

#203483

Purpose

For Agrobacterium transformation. A plastid transit peptide fused mCherry-UnaG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Depositor

Yutaka Kodama

Publication

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

Plastid transit peptide fused mCherry-UnaG

Expression

Bacterial and Plant

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Mesp2

Plasmid

#72693

Purpose

Doxycyline regulated expression of Mesp2 in mammalian cells

Depositor

Timothy Kamp

Publication

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Mesp 2 (Mesp2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GLRX2

Plasmid

#38865

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

GLRX2 (Glrx2 Human)

Tags

His Tev

Expression

Bacterial

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pHL1632

Plasmid

#62829

Purpose

Lim Lab plasmid: AmpR + PLlacO-1::RBS(st7) gfp::Asp terminator + ColE1

Depositor

Han Lim

Publication

Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

Insert

gfp

Use

Synthetic Biology

Tags

st7 RBS

Expression

Bacterial

Promoter

pLlacO-1

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
p413 TEF NAPstar1

Plasmid

#232738

Purpose

Expresses NADPH/NADP+ biosensor NAPstar1 in S. cerevisiae.

Depositor

Bruce Morgan

Publication

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

NAPstar1

Expression

Yeast

Available since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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p413 TEF NAPstar6

Plasmid

#232742

Purpose

Expresses NADPH/NADP+ biosensor NAPstar6 in S. cerevisiae.

Depositor

Bruce Morgan

Publication

Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

Insert

NAPstar6

Expression

Yeast

Available since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCYP10

Plasmid

#212843

Purpose

Expresses the Acidovorax avenae subsp. Avenae LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Publication

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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GLRX5

Plasmid

#39007

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

GLRX5 (GLRX5 Human)

Tags

His Tev

Expression

Bacterial

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pCarCBADE

Plasmid

#117757

Purpose

Codon optimized CarCBADE enzymes from Pectobacterium carotovorum in pBbaA5k backbone

Depositor

Greg Bokinsky

Publication

Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

Insert

Codon optimised CarCBA, Protein CarD, Ferredoxin CarE

Expression

Bacterial

Promoter

PLac(UV5)

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pTRC-APX(W41F) dimeric

Plasmid

#58499

Purpose

Bacterial expression of APX W41F inducible by IPTG

Depositor

Alice Ting

Publication

Dwyer et al Proc Natl Acad Sci U S A. 2014 May 20;11

Insert

pea APX W41F (dimeric)

Tags

His6

Expression

Bacterial

Mutation

W41F

Promoter

trc

Available since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only

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GLRX3

Plasmid

#39090

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

GLRX3 (GLRX3 Human)

Tags

His Tev

Expression

Bacterial

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pLRD18

Plasmid

#208270

Purpose

Production of ent-isokaurene in E. coli MEV20

Depositor

Christopher Voigt

Publication

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

ptmT2
osKSL6

Expression

Bacterial

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCfB4613

Plasmid

#126752

Purpose

Integrate pTRX2_5xUAS-CDC14-tADH1 into yeast genome EasyClone site X-4 with SpHIS5 marker

Depositor

Michael Jensen

Publication

Zhang et al ACS Synth Biol. 2016 Dec 16;5(12):1546-1

Insert

CDC14 (CDC14 Budding Yeast)

Use

Integrative plasmid

Expression

Yeast

Promoter

pTRX2_5xUAS

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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EcYbbN pET15b

Plasmid

#60690

Purpose

Recombinant protein expression of YbbN

Depositor

Mark Wilson

Publication

Lin et al J Biol Chem. 2011 Jun 3;286(22):19459-69

Insert

YbbN

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
GLRX3

Plasmid

#39102

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

GLRX3 (GLRX3 Human)

Tags

Tev His Flag

Expression

Bacterial

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only