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We narrowed to 34 results for: dox

Showing: 1 - 20 of 34 results

  1. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...presence of Dox. In this case, your gene is repressed in steady state and is expressed when Dox is added ...absence of tetracycline or a derivative such as Doxycycline (Dox), the Tet-controlled transactivator (tTA, ...activates the expression of your gene. The addition of Dox in the culture will then repress your gene of interest...activity and it requires continuous administration of Dox to repress transgene expression. In contrast, in ...

  2. Tetracycline Inducible Expression

    Type
    Collection
    ...systems. Dox binds with high affinity to TetR and derivatives like rTetR, rtTA, and tTA. Dox has good ...downstream gene. If tet or one of its analogs like doxycycline (dox) is present, TetR binds to tetracycline and...find plasmids for your experiments using tet- or dox-inducible systems. Plasmid...upstream of a minimal CMV promoter, and other tet- or dox-dependent promoters (sometimes generally called Ptet...relatively inexpensive. The induction profiles for dox and other tetracycline derivatives can be found in...other applications, or search our collection for all dox-regulated plasmids . Looking for viral preps? Find...tetracycline systems or search our collection for all dox-regulated plasmids . ID Plasmid Description PI 63800...

  3. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    ...two PiggyBac integratable cassettes, both with doxycycline inducible promoters (Fig. 2a). The first cassette...contrast. In all the cell lines we tested, 1 µg/mL doxycycline was sufficient to saturate expression. When ...Next, perform a short (12-hour) induction with doxycycline prior to FACS, gating for the top quartile (or...producing cells. After FACS, return the cells to doxycycline-free media and expand the cells. Use a subset...engineered cells via regular intraperitoneal doxycycline injections to enable their tracking with ultrasound...

  4. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    ...from Abhi Aggarwal.   Brandán Pedre Pérez: Redox biosensors Brandán Pedre Pérez is a postdoc at ...Cancer Research Center (DKFZ) who has developed new redox sensitive GFP2 (roGFP2)-based biosensors for live...cysteines near the chromophore that are susceptible to redox modification, the MPST hydrosulfide can be transferred...improved biosensors or biosensors that detect new redox metabolites are vital for researchers to better ...

  5. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    ...genome editing system that could be regulated by doxycycline. Now, they’ve developed another AAV toolkit -...system is regulated not only temporally with doxycycline, but it can also be regulated spatially with ...

  6. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...expression by administering an inducing agent like doxycycline (Favre et al., 2002). They are useful when you...Tetracycline-Dependent Transactivator Correlates with Long-Term Doxycycline-Regulated Transgene Expression in Nonhuman Primates...

  7. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...of ER thiol redox. J Cell Biol. 2013 Apr 15;201(2):337-49. David Ron Redox/ROS Thiol redox-sensitive ratiometric... Cohen Redox/ROS Red fluorescent biosensor for glutathione redox changes Red fluorescent redox-sensitive...Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe. Nat ... with peroxiredoxin-based probes Real-time monitoring of basal H 2 O 2 levels with peroxiredoxin-based... Belousov NADH/NAD+ Peredox Fluorescent biosensor of the cytosolic NADH/NAD+ redox state Imaging Cytosolic... NADH/NAD+ Peredox fluorescent sensor protein for NADH/NAD+ ratio Cytosolic NADH-NAD Redox Visualized ...biosensor Peredox-mCherry in plants In Vivo NADH/NAD+ Biosensing Reveals the Dynamics of Cytosolic Redox Metabolism...

  8. Plasmids for Stem Cell Research

    Type
    Collection
    ...;318(5858):1917-20. Thomson Lentivirus Human Doxycycline-inducible expression of human Oct4, Sox2, Klf4...Cibelli Lentivirus Human Single polycistronic, doxycycline-inducible lentiviral vector for the expression...322(5903):945-9. Hochedlinger Lentivirus Mouse Doxycycline-inducible expression of mouse Oct4, Sox2, Klf4...-9. Jaenisch Lentivirus Mouse Polycistronic, doxycycline-inducible lentiviral vectors for the expression... 6. 106(1):157-62. Jaenisch Lentivirus Mouse Doxycycline-inducible lentiviral expression of mouse Oct4...Aug 25. 126(4):663-76. Yamanaka piggyBac Mouse Doxycycline-inducible piggyBac transposon reprogramming system... 2011 Mar;21(3):518-29. Cheng piggyBac Mouse Doxycycline-inducible piggyBac transposon reprogramming system...

  9. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...control of the TRE promoter, which allows for doxycycline-inducible expression. The end result is genomic...controlled expression of the dCas9-VPR transgene by doxycycline in your target cells. This plasmid adds to the...

  10. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...ubiquitous and constitutive promoter. Use this for doxycycline inducible (tet-off) gene expression! We've also...

  19. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...the Cre inducible (such as with tamoxifen or doxycycline), the Cre recombinase can be expressed only in...
Showing: 1 - 20 of 34 results