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Addgene

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We narrowed to 25 results for: dox

Showing: 1 - 20 of 25 results

  1. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...presence of Dox. In this case, your gene is repressed in steady state and is expressed when Dox is added ...absence of tetracycline or a derivative such as Doxycycline (Dox), the Tet-controlled transactivator (tTA, ...activates the expression of your gene. The addition of Dox in the culture will then repress your gene of interest...activity and it requires continuous administration of Dox to repress transgene expression. In contrast, in ...

  2. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...two PiggyBac integratable cassettes, both with doxycycline inducible promoters (Fig. 2a). The first cassette...contrast. In all the cell lines we tested, 1 µg/mL doxycycline was sufficient to saturate expression. When ...Next, perform a short (12-hour) induction with doxycycline prior to FACS, gating for the top quartile (or...producing cells. After FACS, return the cells to doxycycline-free media and expand the cells. Use a subset...engineered cells via regular intraperitoneal doxycycline injections to enable their tracking with ultrasound...

  3. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    Published
    Aug. 5, 2021, 12:15 p.m.
    ...from Abhi Aggarwal.   Brandán Pedre Pérez: Redox biosensors Brandán Pedre Pérez is a postdoc at ...Cancer Research Center (DKFZ) who has developed new redox sensitive GFP2 (roGFP2)-based biosensors for live...cysteines near the chromophore that are susceptible to redox modification, the MPST hydrosulfide can be transferred...improved biosensors or biosensors that detect new redox metabolites are vital for researchers to better ...

  4. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...genome editing system that could be regulated by doxycycline. Now, they’ve developed another AAV toolkit -...system is regulated not only temporally with doxycycline, but it can also be regulated spatially with ...

  5. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...expression by administering an inducing agent like doxycycline (Favre et al., 2002). They are useful when you...Tetracycline-Dependent Transactivator Correlates with Long-Term Doxycycline-Regulated Transgene Expression in Nonhuman Primates...

  6. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...control of the TRE promoter, which allows for doxycycline-inducible expression. The end result is genomic...controlled expression of the dCas9-VPR transgene by doxycycline in your target cells. This plasmid adds to the...

  7. New Viral Vectors - Summer 2024

    Type
    Blog Post
    Published
    July 9, 2024, 1:15 p.m.
    ...ubiquitous and constitutive promoter. Use this for doxycycline inducible (tet-off) gene expression! We've also...

  12. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ... per generation, and (2) the addition of the thioredoxin-binding domain (TBD) from bacteriophage T7 DNA...

  13. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...target cells by the addition of tetracycline/doxycycline to the grown media resulting in target gene knockdown...

  16. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...the Cre inducible (such as with tamoxifen or doxycycline), the Cre recombinase can be expressed only in...

  19. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...also choose to add smaller proteins, such as Thioredoxin A (TrxA) that improve disulfide bond formation...

  20. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...vector like pLenti CMV Puro DEST (w118-1) or the doxycycline-inducible pLIX_403. The chosen attR destination...
Showing: 1 - 20 of 25 results