We narrowed to 1,640 results for: plasmid for e coli

1. USP7 G392D catalytic

   Plasmid

   #227203

   Purpose

   Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   G392D; aa 207-560

   Promoter

   T7-promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. F118L USP7 TRAF

   Plasmid

   #227228

   Purpose

   Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   F118L; aa 62-205

   Promoter

   T7-promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. L469R FL USP7

   Plasmid

   #227225

   Purpose

   Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   L469R

   Promoter

   T7-promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. E345K USP7 catalytic

   Plasmid

   #227211

   Purpose

   Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   E345K; aa 207-560

   Promoter

   T7-promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. L373F USP7 catalytic

   Plasmid

   #227207

   Purpose

   Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   L373F; aa 207-560

   Promoter

   T7-promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. G392A USP7 catalytic

   Plasmid

   #227204

   Purpose

   Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   G392A; aa 207-560

   Promoter

   T7-promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. E345K FL USP7

   Plasmid

   #227224

   Purpose

   Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   E345K

   Promoter

   T7-promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. V485G FL USP7

   Plasmid

   #227222

   Purpose

   Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   V485G

   Promoter

   T7-promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pORTMAGE312B

   Plasmid

   #128969

   Purpose

   Broad-host-range KanR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid induction

   Depositor

   George Church

   Article

   Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

   Inserts

   PapRecT
   mutL E32K
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E32K

   Promoter

   xylS-Pm

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pOK12

    Plasmid

    #49789

    Purpose

    E. coli cloning vector (KanR, low copy, blue/white selection)

    Depositor

    Joachim Messing

    Article

    Vieira et al Gene. 1991 Apr;100:189-94.

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    lacZ

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNBU2_erm_SIE1

    Plasmid

    #136356

    Purpose

    Selection by Inducible Effectors Test Plasmid

    Depositor

    Andrew Goodman

    Article

    Bencivenga-Barry et al J Bacteriol. 2020 Jan 15;202(3). pii: JB

    Inserts

    Type VI effector bfe1
    Type VI effector bte1
    BT_4676
    tetR
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    ATG removed to add signal sequence before gene

    Promoter

    Available since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKD077

    Plasmid

    #136473

    Purpose

    SSB-mTur2 IDL fusion expression plasmid

    Depositor

    James Keck

    Article

    Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60

    Insert

    SSB-mTur2 (ssb E. coli)
    

    Use

    Tags

    mTurquoise2

    Expression

    Bacterial

    Mutation

    mTurquoise2 inserted between Phe148 and Ser149

    Promoter

    T7 promoter

    Available since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMM1162

    Plasmid

    #112535

    Purpose

    pZE2-hrtR-RBS3-chuA - LuxCDABE transcriptional unit deletion of plasmid pMM643, ColE1 origin, KanR

    Depositor

    Timothy Lu

    Article

    Mimee et al Science. 2018 May 25;360(6391):915-918.

    Inserts

    HrtR
    ChuA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23107 and ProD

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSCL.040

    Plasmid

    #184974

    Purpose

    Test effect of extending a1/a2 on ADE2 editing rates in yeast

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    ADE2 donor P272stop, a1/a2 length extended to 27 …

    Promoter

    Gal7

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSCL.109

    Plasmid

    #184978

    Purpose

    Test effect of extending a1/a2 on LYP1 editing rates in yeast

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 editing ncRNA and gRNA, LYP1 E27X, a1/a2 length: 27 v1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    LYP1 donor E27stop, a1/a2 length extended to 27 bp

    Promoter

    Gal7

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSCL.107

    Plasmid

    #184976

    Purpose

    Test effect of extending a1/a2 on CAN1 editing rates in yeast

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 editing ncRNA and gRNA, CAN1 G444X, a1/a2 length: 27 v1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    CAN1 donor G444stop, a1/a2 length extended to 27 …

    Promoter

    Gal7

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSCL.111

    Plasmid

    #184980

    Purpose

    Test effect of extending a1/a2 on TRP2 editing rates in yeast

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 27 v1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    TRP2 donor E64stop, a1/a2 length extended to 27 bp

    Promoter

    Gal7

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSCL.113

    Plasmid

    #184982

    Purpose

    Test effect of extending a1/a2 on FAA1 editing rates in yeast

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 27 v1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    FAA1 donor P233stop, a1/a2 length extended to 27 …

    Promoter

    Gal7

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSCL.039

    Plasmid

    #184973

    Purpose

    Test effect of extending a1/a2 on ADE2 editing rates in yeast

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    ADE2 donor P272stop, a1/a2 length extended to 27 …

    Promoter

    Gal7

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pQE31-6xHis-pHCountdown

    Plasmid

    #182300

    Purpose

    E. coli plasmid encoding pH-Countdown with a hexahistidine tag

    Depositor

    Andrew York

    Article

    Seidel et al (2021), doi: 10.5281/zenodo.5810930

    Insert

    pH Countdown
    

    Use

    Tags

    histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge