Addgene

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pDONR221-ACE2

Plasmid

#158438

Purpose

Gateway Cloning compatible entry vector for the human ACE2 gene.

Depositor

Alejandro Chavez, Sho Iketani

Insert

ACE2 (ACE2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMtBS

Plasmid

#32226

Depositor

Christopher Buck

Article

Schowalter et al PLoS Pathog. 2011 Jul;7(7):e1002161. Epu

Insert

MCV Small t antigen

Use

Tags

Expression

Mammalian

Mutation

Splice site was silently mutated, and a restricti…

Promoter

Available since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only

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PET30B-PTEN

Plasmid

#20741

Depositor

Alonzo Ross

Article

Redfern et al Biochemistry. 2008 Feb 19. 47(7):2162-71

Insert

PTEN (PTEN Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

April 2, 2009

Availability

Academic Institutions and Nonprofits only
599 p3E-mcs1

Plasmid

#49004

Purpose

Multiple cloning site in 3' gateway entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Type

Empty backbone

Use

Gateway 3' entry

Tags

Expression

Mutation

Promoter

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pICSL11137

Plasmid

#191783

Purpose

Kanamycin resistance in plants

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

35S::nptII-T-Nos

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLibacceptorV2

Plasmid

#106250

Purpose

Recombination-mediated cassette exchange with target genomic loci

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pAN3944

Plasmid

#74702

Purpose

Circuit plasmid: 0x1C

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

Circuit 0x1C

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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AgAK

Plasmid

#71290

Purpose

Expression of A. gambiae adenosine kinase

Depositor

Vern Schramm

Article

Cassera et al Biochemistry. 2011 Mar 22;50(11):1885-93

Insert

Adenosine Kinase

Use

Tags

6X Histidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pQacR-Q2

Plasmid

#74690

Purpose

QacR-Q2 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSQ2-QacR-PQacR-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pAN3945

Plasmid

#74699

Purpose

Circuit plasmid: 0x41

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

Circuit 0x41

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAN4037

Plasmid

#74703

Purpose

Output plasmid: PPhlF-PBetI-YFP

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PPhlF-PBetI-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pET28b-PaODH

Plasmid

#110802

Purpose

Expresses PaODH and opine dehydrogenase from Pseudomonas aeruginosa

Depositor

Audrey Lamb

Article

McFarlane et al Biochemistry. 2017 Nov 14;56(45):5967-59

Insert

Pa4835

Use

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAN4023

Plasmid

#74701

Purpose

Output plasmid: PBM3R1-YFP

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PBM3R1-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pmEOS2-VSVG

Plasmid

#98286

Purpose

mammalian expression of VSVG-mEOS2

Depositor

Mike Heilemann

Article

Fricke et al Sci Rep. 2015 Sep 11;5:14072. doi: 10.10

Insert

VSVG

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pBXC3GH

Plasmid

#47070

Purpose

FX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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His-NKX3.1

Plasmid

#42896

Depositor

Edward Gelmann

Article

Ju et al Biochemistry. 2009 Nov 10;48(44):10601-7

Insert

NKX3.1 (NKX3-1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

April 9, 2013

Availability

Academic Institutions and Nonprofits only
pQacR-Q1

Plasmid

#74689

Purpose

QacR-Q1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSQ1-QacR-PQacR-YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pSARM-luciferase

Plasmid

#67794

Purpose

Use together with VSV-G to package M-PMV reporter virus

Depositor

Stephen Goff

Article

Wang et al J Virol. 2015 Mar;89(5):2813-9. doi: 10.

Insert

firefly luciferase (gag )

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only
pICXNH3

Plasmid

#49020

Purpose

FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag and 3C protease cleavage site

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Yeast

Mutation

Promoter

AOX1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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Flag-Paxillin

Plasmid

#15212

Depositor

Rick Horwitz

Article

Webb et al J Cell Sci. 2005 Nov 1. 118(Pt 21):4925-

Insert

Paxillin (PXN Chicken)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only