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pcDNA3-mPC1_4148-3HA

Plasmid

#108398

Purpose

Express cytoplasmic tail mouse PC1 from amino acid 4148 to 4293

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

Pkd1 (Pkd1 Mouse)

Use

Tags

3HA

Expression

Mammalian

Mutation

variant ID rs13468401 missense variant R4237Q

Promoter

CMV

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mPC1_4148-eGFP

Plasmid

#108397

Purpose

Express cytoplasmic tail mouse PC1 from amino acid 4148 to 4293

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

Pkd1 (Pkd1 Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

variant ID rs13468401 missense variant R4237Q

Promoter

CMV

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mPC1_4148-eGFP-3HA

Plasmid

#108396

Purpose

Express cytoplasmic tail mouse PC1 from amino acid 4148 to 4293

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

Pkd1 (Pkd1 Mouse)

Use

Tags

eGFP-3HA

Expression

Mammalian

Mutation

variant ID rs13468401 missense variant R4237Q

Promoter

CMV

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3 SIRT1-NTerm Exon1-Exon3

Plasmid

#105681

Purpose

Bacterial Expression construct of N terminus of SIRT1: Exon1-Exon3 (deltaExon2)

Depositor

Ullas Kolthur-Seetharam

Article

Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

Insert

SIRT1-NTerm: Exon1-Exon3 (Sirt1 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Synonymous base changes not affecting the protein…

Promoter

tac_Promoter

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3- SIRT1-NTerm Exon1-Exon2-Exon3

Plasmid

#105680

Purpose

Bacterial Expression construct of N terminus of SIRT1: Exon1-Exon2-Exon3

Depositor

Ullas Kolthur-Seetharam

Article

Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

Insert

SIRT1-NTerm: Exon1-Exon2-Exon3 (Sirt1 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Synonymous base changes not affecting the protein…

Promoter

tac_Promoter

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe 3XFLAG-T34A-GATA6-3XAU1 puro

Plasmid

#72609

Purpose

For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single Threonine#34 to alanine mutation

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Retroviral

Tags

3XAU1 and 3XFLAG

Expression

Mammalian

Mutation

changed threonine34 to alanine

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
pBabe 3XFLAG-S33A-GATA6-3XAU1 puro

Plasmid

#72610

Purpose

For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single serine#33 to alanine mutation

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Retroviral

Tags

3XAU1 and 3XFLAG

Expression

Mammalian

Mutation

changed serine33 to alanine

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
pET-28b-RfxCas13d-His

Plasmid

#141322

Purpose

Plasmid for bacterial expression and purification of RfxCas13d protein

Depositor

Miguel Angel Moreno-Mateos, Ariel Bazzini

Article

Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

Insert

Rfx-Cas13d

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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Fucci(CA) hCdt1-iRFP hGeminin-TagBFP2

Plasmid

#190181

Purpose

Fluorescent reporter vector to visualize all of cell cycle phases.

Depositor

Toshiro Sato

Article

Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

Inserts

iRFP
hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
TagBFP2
hGeminin(1/110)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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Gephyrin scFv [L106/23]

Plasmid

#206775

Purpose

Mammalian Expression of Gephyrin scFV. Derived from hybridoma L106/23 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Gephyrin (Homo sapiens) recombinant scFV (GPHN Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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