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Fucci(CA) hCdt1-iRFP hGeminin-iRFP

Plasmid

#190182

Purpose

Fluorescent reporter vector for visualization of G0 and other cell cycle phases.

Depositor

Toshiro Sato

Article

Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

Inserts

iRFP
hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
iRFP
hGeminin(1/110)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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IP3 receptor, type 1 scFv [L24/21]

Plasmid

#206790

Purpose

Mammalian Expression of IP3 receptor, type 1 scFV. Derived from hybridoma L24/21 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

IP3 receptor, type 1 (Rattus norvegicus) recombinant scFV (Itpr1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19_HDRT_TRAC_CD19-CAR

Plasmid

#183473

Purpose

Encodes for a homology-directed DNA repair template for the insertion of a second generation CD19-specific CAR into the TRAC gene

Depositor

Dimitrios Wagner

Article

Kath et al Mol Ther Methods Clin Dev. 2022 Apr 12;2

Inserts

TRAC (TRAC Human)
CD19-specific CAR transgene insert
TRAC (TRAC Human)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pInducer-myc-ERK1-R84S/S170D

Plasmid

#213593

Purpose

Doxycycline inducible expression of myc-tagged ERK1-R84S/S170D cDNA in mammilian cells

Depositor

Channing Der

Article

Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

Insert

MAPK3 (MAPK3 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

R84S, S170D

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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VGAT scFv [L118/14]

Plasmid

#206754

Purpose

Mammalian Expression of VGAT scFV. Derived from hybridoma L118/14 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

VGAT (Mus musculus) recombinant scFV (Slc32a1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-SPHKAP [L131/17R]

Plasmid

#177477

Purpose

Mammalian Expression Plasmid of anti-SPHKAP (Mouse). Derived from hybridoma L131/17.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-SPHKAP (Mus musculus) recombinant mouse monoclonal antibody (Sphkap Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-RPS14(WT)

Plasmid

#122297

Purpose

expresses RPS14 protein with a flag tag

Depositor

Gerardo Ferbeyre

Article

Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

Insert

RPS14 (RPS14 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+FLAG-KrascomG12D

Plasmid

#206844

Purpose

To express mouse Kras encoded by common mouse codons (to increase expression) and with a G12D mutation with an N-terminal FLAG tag.

Depositor

Christopher Counter

Article

Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

Insert

Kras (Kras Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Codons altered to most optimum based on mouse cod…

Promoter

Available since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pT3TS-RfxCas13d-NLS-HA

Plasmid

#141321

Purpose

Plasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)

Depositor

Miguel Angel Moreno-Mateos, Ariel Bazzini

Article

Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

Insert

Rfx-Cas13d (NLS-RfxCas13d-NLS)

Use

CRISPR

Tags

HA and NLS

Expression

Mutation

Promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-Neurofascin (extracellular) [A12/18R]

Plasmid

#177438

Purpose

Mammalian Expression Plasmid of anti-Pan-Neurofascin (extracellular) (Rat). Derived from hybridoma A12/18.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Pan-Neurofascin (extracellular) (Rattus norvegicus) recombinant mouse monoclonal antibody (Nfasc Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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