We narrowed to 9,520 results for: try

1. pYPQ292 (AaCas12b)-D570A-TV

   Plasmid

   #136380

   Purpose

   CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AaCas12b (D570A)-TV
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D570A

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pG418R-GMRWhite

   Plasmid

   #165883

   Purpose

   G418 resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Transgenesis Ready Selection Vector
   

   Use

   Synthetic Biology

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBP-ORF

   Plasmid

   #72949

   Purpose

   Entry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction site

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Level 0 - ORFs
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEnt R3L2 TetO(sh) mCh-Rs1-2

   Plasmid

   #24417

   Depositor

   Edward Hsiao

   Article

   Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

   Insert

   Rs1
   

   Use

   Entry vector

   Tags

   FLAG and mCherry

   Mutation

   Human 5HT4B receptor with D100A mutation, generat…

   Available Since

   March 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCI-Flag-NEDD4L∆WW2

   Plasmid

   #166708

   Purpose

   expresses NEDD4L with inactivating mutation in second WW domain in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

   Insert

   NEDD4L W453A
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed tryptophan 453 to alanine

   Promoter

   CMV promoter

   Available Since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCI-Flag-NEDD4L∆WW3

   Plasmid

   #166709

   Purpose

   expresses NEDD4L with inactivating mutation in third WW domain in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

   Insert

   NEDD4L W525A
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed tryptophan 525 to alanine

   Promoter

   CMV promoter

   Available Since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYPQ255

   Plasmid

   #124310

   Purpose

   Gateway entry vector with rAPOBEC1 and pcoCas9D10A for C-T base editing

   Depositor

   Yiping Qi

   Article

   Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

   Insert

   rAPOBEC1-pcoCas9D10A-UGI
   

   Use

   CRISPR

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pENTR_Hu_Plk5(WT)

   Plasmid

   #136329

   Purpose

   Human Plk5 WT in Gateway pENTR

   Depositor

   Guillermo de Cárcer, Marcos Malumbres

   Article

   de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

   Insert

   PLK5 (PLK5 Human)
   

   Use

   Gateway entry vector

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p3x-Flag Gli3 37R3-2

   Plasmid

   #120305

   Purpose

   Expresses human protein Gli containing a 4HT-inducible intein evolved to operate at 37C

   Depositor

   David Liu

   Article

   Peck et al Chem Biol. 2011 May 27;18(5):619-30. doi

   Insert

   Intein 37R3-2, Gli3
   

   Use

   Synthetic Biology

   Tags

   3X Flag

   Expression

   Mammalian

   Mutation

   Contains mutations as reported in 2011 Chemistry …

   Promoter

   CMV

   Available Since

   Feb. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pENTR4_FLAG/HA_EWSR1_His6

    Plasmid

    #26364

    Depositor

    Thomas Tuschl

    Article

    Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    Entry vector

    Tags

    FLAG/HA and His6

    Mutation

    delta 657 (stop codon)

    Available Since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only