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pME nls BFP FLAG WPRE bGH pA (JDW 484)

Plasmid

#224539

Purpose

A Gateway compatible middle entry clone containing a 3x-NLS-mTagBFP2-FLAG for labeling the nucleus

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

nls-mTagBFP2-FLAG_WPRE-bGH-pA

Use

Gateway Cloning

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-WPRE-bGH-pA (JDW 1221)

Plasmid

#224527

Purpose

A Gateway compatible 3' entry clone containing a WPRE upstream of Bovine growth hormone polyA to stabilize mRN

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

WPRE-bGH

Use

Gateway Cloning

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pME_Golgi-BFP_P2A_H2A_tdiRFP (JDW 1079)

Plasmid

#224491

Purpose

Gateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-tdiRFP (BFP golgi reporter and tandem infrared RFP nuclear reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Golgi-mTagBFP-P2A-H2A-tdiRFP

Use

Gateway Cloning

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pME-rox-nls-mCherry-V5-2xStop-rox (JDW 1167)

Plasmid

#224524

Purpose

A Gateway compatible middle entry clone containing a Rox flanked 3xNLS-mCherry-V5-2xStop cassette

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

rox-3xNLS-mCherry-V5-2xSTOP-rox

Use

Gateway Cloning

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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p5E lexOP-c-fos min pro (JDW 1254)

Plasmid

#224477

Purpose

Gateway compatible 5' entry clone containing a Lex Operon upstream of a c-fos minimal promoter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexOp

Use

Gateway Cloning

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-dr_Unc (JDW 1365)

Plasmid

#224482

Purpose

Gateway compatible 5' entry clone containing the Zebrafish unc-503 promoter with a minimal c-fos promoter and b-globin intron

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Unc-503 promoter

Use

Gateway Cloning

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pME-myr-mTagBFP2-Flag (JDW 1183)

Plasmid

#224492

Purpose

Gateway compatible middle entry clone containing a Myristoylated mTagBP2 with a c-terminal Flag tag (cell membrane BFP marker)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

myr-mTagBFP2-Flag

Use

Gateway Cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-Lifeact-mCherry-3xHA (JDW 1321)

Plasmid

#224493

Purpose

Gateway compatible middle entry clone containing Lifeact-mCherry (RFP F-actin reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Lifeact-mCherry-3xHA

Use

Gateway Cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME nls EGFP-Cre (JDW 1329)

Plasmid

#224515

Purpose

A Gateway compatible middle entry clone containing a NLS-EGFP-Cre fusion

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

nls-EGFP-Cre

Use

Cre/Lox

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME iCre-I (JDW 1203)

Plasmid

#224520

Purpose

A Gateway compatible middle entry clone containing a loxP flanked, self inactivating Cre that contains an intron to prevent recombination in bacteria

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

iCre-I

Use

Cre/Lox

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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