We narrowed to 2,200 results for: dcas9

1. BB1_23_MS2bind_VP64_SV40NLS

   Plasmid

   #161479

   Purpose

   BB1 encoding a MS2 binding VP64 fusion protein with an additional SV40 NLS

   Depositor

   Brigitte Gasser, Diethard Mattanovich

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Insert

   MS2 binding protein + VP64 activation domain
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMM587

   Plasmid

   #133593

   Purpose

   Part Plasmid for dCas9, Part 3b(coding sequence)

   Depositor

   Megan McClean

   Article

   Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

   Insert

   dCas9
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMM586

   Plasmid

   #133592

   Purpose

   Part Plasmid for dCas9, Part 3(coding sequence)

   Depositor

   Megan McClean

   Article

   Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

   Insert

   dCas9
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMM729

   Plasmid

   #133598

   Purpose

   Part Plasmid for Mxi1-dCas9, Part 3(coding sequence)

   Depositor

   Megan McClean

   Article

   Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

   Insert

   Mxi1-dCas9
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMM767

   Plasmid

   #133604

   Purpose

   Part Plasmid for dCas9 NLS, Part 3(coding sequence)

   Depositor

   Megan McClean

   Article

   Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

   Insert

   dCas9 NLS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMM585

   Plasmid

   #133591

   Purpose

   Part Plasmid for dCas9, Part 3a(coding sequence)

   Depositor

   Megan McClean

   Article

   Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

   Insert

   dCas9
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMM768

   Plasmid

   #133605

   Purpose

   Part Plasmid for dCas9 NLS, Part 3b(coding sequence)

   Depositor

   Megan McClean

   Article

   Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

   Insert

   dCas9 NLS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMM769

   Plasmid

   #133606

   Purpose

   Part Plasmid for dCas9 NLS, Part 3a(coding sequence)

   Depositor

   Megan McClean

   Article

   Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

   Insert

   dCas9 NLS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA

   Plasmid

   #85756

   Purpose

   All in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).

   Depositor

   Lawrence Stanton

   Article

   Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

   Inserts

   Csy4-T2A-SpRFN-P2A-GFP
   gRNA
   

   Use

   CRISPR

   Tags

   Csy4, Csy4 recognition sequence, FokI fusion via …

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CMV and U6

   Available Since

   Jan. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLY70

    Plasmid

    #130948

    Purpose

    A CRISPR activation device with the necessary genes (dxcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter PrhaB), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dxcas9 (3.7)
    tetR
    rhaS
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9 for dCas9; Sy…

    Promoter

    Pcon, PpspA-LEA3B3, PrhaB, and Ptet

    Available Since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLY164

    Plasmid

    #184140

    Purpose

    Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 3)

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Pcon, PpspA-R3, PrhaB, and Ptet

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLY61

    Plasmid

    #130928

    Purpose

    A CRISPR activation device with the necessary genes (dxcas9 3.7 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dxcas9 (3.7)
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9 for dCas9; Sy…

    Promoter

    Anderson promoter: J23106, PpspA-LEA1B1, and Ptet

    Available Since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLY162

    Plasmid

    #184138

    Purpose

    Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 1)

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Pcon, PpspA-R1, PrhaB, and Ptet

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLY163

    Plasmid

    #184141

    Purpose

    Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 2)

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Pcon, PpspA-R2, PrhaB, and Ptet

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLY168

    Plasmid

    #184142

    Purpose

    Reporter circuit for hijacking mRNA of arsenic responsive gene cluster as CRISPR RNA (site 2)

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Pcon, PpspA-Ar2, PrhaB, and Ptet

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMCP-RecT

    Plasmid

    #183827

    Purpose

    pLenti-EF1A-MCP-EXTEN-RecT-NLS

    Depositor

    Le Cong

    Article

    Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

    Insert

    MCP-RecT_NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available Since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ampl43

    Plasmid

    #161830

    Purpose

    pRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.

    Depositor

    Saeed Tavazoie

    Article

    Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAC-SP6

    Plasmid

    #102418

    Purpose

    Ac transposase with SP6 promoter for in vitro transcription

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    Ac transposase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    SP6

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMCRi

    Plasmid

    #207524

    Purpose

    Plasmid for CRISPRi; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR

    Depositor

    Pablo Ivan Nikel

    Article

    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

    Insert

    xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR
    

    Use

    Pseudomonas vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMT423

    Plasmid

    #139382

    Purpose

    Constitutively expressed dCas9, LacI and TetR, pNBU2 backbone, AmpR, TetR

    Depositor

    Christopher Voigt

    Article

    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

    Insert

    Constitutively expressed dCas9, LacI and TetR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    P1

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge