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B9VQB0_ARTSF pSecTag2(pm) (pBS0257)

Plasmid

#185146

Purpose

For the mammalian expression of the brine shrimp protein B9VQB0_ARTSF. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

B9VQB0_ARTSF

Expression

Mammalian

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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A0A1D1UNQ6_RAMVA pSecTag2(pm) (pBS0256)

Plasmid

#185145

Purpose

For the mammalian expression of the tardigrade protein A0A1D1UNQ6_RAMVA. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

A0A1D1UNQ6_RAMVA

Expression

Mammalian

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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Dehydrin_Q84N45_9LILI pSecTag (pBS0255)

Plasmid

#185144

Purpose

For the mammalian expression of the xerophyta (Xerophyta zambiana) protein Dehydrin_Q84N45_9LILI. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

Dehydrin_Q84N45_9LILI

Expression

Mammalian

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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LEA1_APHAV_Chakrabortee pSecTag (pBS0254)

Plasmid

#185143

Purpose

For the mammalian expression of the nematode (Aphelenchus avenae) protein LEA1_APHAV. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

LEA1_APHAV

Expression

Mammalian

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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MAHS_RAMVA_Tanaka pSecTag (pBS0253)

Plasmid

#185142

Purpose

For the mammalian expression of the tardigrade protein MAHS_RAMVA. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

MAHS_RAMVA

Expression

Mammalian

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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CAHS7_HYPDU_Boothby pSecTag (pBS0251)

Plasmid

#185141

Purpose

For the mammalian expression of the tardigrade protein CAHS7_HYPDU. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS7_HYPDU

Expression

Mammalian

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
SAHS10_A0A1D1UJR2_RAMVA pSecTag (pBS0250)

Plasmid

#185140

Purpose

For the mammalian expression of the tardigrade protein SAHS1_A0A1D1UJR2. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

SAHS1_A0A1D1UJR2

Expression

Mammalian

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-nCoV2019-N-YBBR-2xStrep-IRES-Puro

Plasmid

#185450

Purpose

Expresses SARS-CoV-2 nucleocapsid protein with a c-terminal YBBR and Strep tag in mammalian cells

Depositor

Ahmet Yildiz

Article

Jack et al PLoS Biol. 2021 Oct 11;19(10):e3001425.

Insert

Nucleocapsid (N SARS-CoV-2)

Use

Lentiviral

Tags

Strep-Tag II and YBBR

Expression

Mammalian

Promoter

EF1alpha

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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ApoE3_D125I_mCh-2xFKBP (pBS1157)

Plasmid

#185330

Purpose

For the mammalian expression of the human protein ApoE3_D125I attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE3_D125I

Expression

Mammalian

Mutation

D125I

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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ApoE3_mCh-2xFKBP (pBS1156)

Plasmid

#185329

Purpose

For the mammalian expression of the human protein ApoE3 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE3

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
ApoE3_D125I_mCh-SspB (pBS1145)

Plasmid

#185327

Purpose

For the mammalian expression of the human protein ApoE3_D125I attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE3_D125I

Expression

Mammalian

Mutation

D125I

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
ApoE4_mCh-SspB (pBS1143)

Plasmid

#185325

Purpose

For the mammalian expression of the human protein ApoE4 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE4

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CAHS1_PARRC_101-189_mCh-2xFKBP (pBS1136)

Plasmid

#185320

Purpose

For the mammalian expression of the tardigrade protein CAHS1_PARRC_101-189 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS1_PARRC_101-189

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
SYUA_HUMAN_mCh-2xFKBP (pBS1135)

Plasmid

#185319

Purpose

For the mammalian expression of the human protein SYUA_HUMAN attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

SYUA_HUMAN

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pvLEA22mer_mutant_3_mCh-2xFKBP (pBS1123)

Plasmid

#185318

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_3 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_3

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PvLEA4_repeats_k5_1_mCh-2xFKBP (pBS1122)

Plasmid

#185317

Purpose

For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k5_1 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k5_1

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pvLEA22mer_mutant_10_mCh-2xFKBP (pBS1121)

Plasmid

#185316

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_10 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_mutant_10

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CAHS2_PARRC_98-130_mCh-2xFKBP (pBS1120)

Plasmid

#185315

Purpose

For the mammalian expression of the tardigrade protein CAHS2_PARRC_98-130 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS2_PARRC_98-130

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PvLEA4_repeats_k3_26_mCh-2xFKBP (pBS1118)

Plasmid

#185313

Purpose

For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k3_26 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k3_26

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
DHN_VrDHN1a_mCh-2xFKBP (pBS1117)

Plasmid

#185312

Purpose

For the mammalian expression of the riverbank grape plant protein DHN_VrDHN1a attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DHN_VrDHN1a

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge