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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,424 results for: NTS

Showing: 1321 - 1340 of 14424 results

  1. pT22-GluCNZ-6His

    Plasmid
    #213504
    Purpose
    Designed for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.
    Depositor
    John Denu
    Article
    Kuznetsov et al J Biol Chem. 2018 Aug 3;293(31):12271-12
    Insert
    Glucanase (glucan endo-1,3-beta-glucosidase)
    Tags
    6His tag
    Expression
    Bacterial
    Mutation
    1-36 Signal Sequence Deletion
    Promoter
    T7
    Available Since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    213504-plasmid-map-sequence-id-424932

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  2. pYPQAT25

    Plasmid
    #223397
    Purpose
    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by AtUBQ10 and sgRNA was driven by AtU3; Hygromycin for plants select.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    AtUBQ10-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223397-plasmid-map-sequence-id-453300

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  7. pGREAT26

    Plasmid
    #170914
    Purpose
    GoldenBraid Double Transcriptional Unit - pEstradiol_Inducible_E-Luc_NtEUt+pNOS_Red-F_NOSt
    Depositor
    Markita Landry
    Article
    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102
    Insert
    E-Luc
    Use
    Luciferase and Synthetic Biology
    Expression
    Plant
    Mutation
    c.375C>T silent mutation to remove BbsI site (…
    Promoter
    pEstradiol_Inducible
    Available Since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    170914-plasmid-map-sequence-id-334493

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  9. pYPQ230-RV

    Plasmid
    #188544
    Purpose
    Gateway entry clone (attL1 & attR5) for LbCas12a-RV mediated genome editing
    Depositor
    Yiping Qi
    Article
    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:
    Insert
    LbCas12a-RV
    Use
    CRISPR; Gateway compatible lbcas12a-rv entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    G146R, R182V
    Available Since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    188544-plasmid-map-sequence-id-369474

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Showing: 1321 - 1340 of 14424 results