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Plasmid#66190Purposeexpression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), basta selectionDepositorInsertCas9
UseTagsExpressionPlantMutationplant-codon optimized with higher GC contents (62…Promotercauliflower mosaic virus 35S promoter (P35S)Available sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-Mts
Plasmid#195065PurposeExpresses FLAG-tagged Drosophila mts proteinDepositorInsertmicrotubule star (mts Fly)
UseTags3xFLAGExpressionInsectMutationPromoterUbi-p63eAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-Pp2A-29B
Plasmid#195066PurposeExpresses FLAG-tagged Drosophila Pp2A-29B proteinDepositorInsertProtein phosphatase 2A at 29B (Pp2A-29B Fly)
UseTags3xFLAGExpressionInsectMutationPromoterUbi-p63eAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMDC43-FIT2-FLL[157-159]AAA
Plasmid#96994PurposeExpress GFP-tagged mutated mouse FIT2 gene in plants.DepositorInsertMmFIT2 (Fitm2 Mouse)
UseTagsGFPExpressionPlantMutationAmino acid residues 157-159 were mutated (FLL[157…Promoter35SAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMDC32-FIT2-FLL[157-159]AAA
Plasmid#96991PurposeExpress mouse FIT2 gene in plants.DepositorInsertMmFIT2 (Fitm2 Mouse)
UseTagsExpressionPlantMutationAmino acid residues 157-159 were mutated (FLL[157…Promoter35SAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-SNAP-hCB2_IRES_EGFP
Plasmid#223506PurposeExpression of CB2 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection markerDepositorInsertSNAP-tag-CNR2 and EGFP (CNR2 Synthetic, Human)
UseTagsSNAP-tagExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-Mts D85N
Plasmid#208403PurposeExpresses FLAG-tagged Drosophila mts protein with D85N mutationDepositorInsertmicrotubule star (mts Fly)
UseTags3xFLAGExpressionInsectMutationD85NPromoterUbi-p63eAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-Mts H59Q
Plasmid#208402PurposeExpresses FLAG-tagged Drosophila mts protein with H59Q mutationDepositorInsertmicrotubule star (mts Fly)
UseTags3xFLAGExpressionInsectMutationH59QPromoterUbi-p63eAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-Mts H241A
Plasmid#208404PurposeExpresses FLAG-tagged Drosophila mts protein with H241A mutationDepositorInsertmicrotubule star (mts Fly)
UseTags3xFLAGExpressionInsectMutationH241APromoterUbi-p63eAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-tws
Plasmid#195068PurposeExpresses FLAG-tagged Drosophila tws proteinDepositorInserttwins (tws Fly)
UseTags3xFLAGExpressionInsectMutationPromoterUbi-p63eAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCX-DI-2A
Plasmid#74671PurposeMammalian expression of DI-2A (hCD73/F2A/hCD39)DepositorUseTagsF2AExpressionMammalianMutationPromoterpCAGGSAvailable sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-membrane-mEGFP
Plasmid#105526PurposeT7 promotor drives in vitro mRNA transcription of a mEGFP-tagged membrane reporterDepositorInsertGNAI2 (GNAI2 Human)
UseIn vitro transcriptionTagsmEGFPExpressionMutationfragment encoding the plasma membrane targeting s…PromoterT7Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
hum alpha ENAC promoter a25-a23
Plasmid#83427Purposepromoter reporter construct to test 5' flanking sequencesDepositorInserthuman aENaC gene 5' flanking seq + 55 nt of the 5' UTR for exon 1A (SCNN1A Human)
UseLuciferaseTagsLuciferaseExpressionMutationPromoteralpha ENACAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p1-GST-ArcΔCTD
Plasmid#119878PurposeBacterial Expression construct for the production and purification of Arc missing C-terminal DomainDepositorInsertActivity Regulated Cytoskeletal Protein (Arc Rat)
UseTagsGSTExpressionBacterialMutationDeletion of nt 832-1110PromotertacAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgNT.3#3/Cre
Plasmid#173662PurposeExpresses a NT.3-targeting gRNA and Cre-recombinaseDepositorInsertNon-targeting gRNA
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgNT.2#2/Cre
Plasmid#173661PurposeExpresses a NT.2-targeting gRNA and Cre-recombinaseDepositorInsertNon-targeting gRNA
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract
Plasmid#138525PurposeExpresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.DepositorInsertControl non-PRC2 binding A-tract repeat RNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-casBCDE(g8)
Plasmid#89727PurposeExpresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing 33-nt spacer (referred to as 'wt') matching a protospacer in the M13 phage genome.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_SNORD27h_2ntmut
Plasmid#73067Purposeexpression clone for human mutated SNORD27 (C/D box snoRNA U27)DepositorInsertSNORD27 (E2F7 Human)
UseTagsno tagExpressionMammalianMutation2 nt mutation in AS box that binds E2F7 pre-mRNAPromoterAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMCh2008_pLenti_Syn_mChe-cdc42E7-E63'UTR
Plasmid#118622Purposeexpression of mChe-tagged cdc42E7-E6-3'UTR under pSyn promoter for primary neuronsDepositorInsertcdc42E7-E6 3'UTR (Cdc42 Mouse)
UseLentiviralTagsmCherryExpressionMammalianMutationchanged ttgcttt to cggtaag (496 - 502 nt in 3…Promotersynapsin I (rat)Available sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only