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Plasmid#219416PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysateDepositorInsertmBlueberry2(-9)
UseTagsMGHHHHHGGExpressionBacterialMutationK137E, K199E, R234EPromoterT7Available sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyCatcher -AVI
Plasmid#223635PurposeProtein purification of SpyCatcher003 (S49C) with AVI and 6xHis tags from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsAVI tag and His6ExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FKBP-empty
Plasmid#221332PurposeProtein overexpression in bacteriaDepositorInsert6xHis-3xFKBP empty scaffold
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ChmN-C47A
Plasmid#221367PurposeExpress C47A ChmN in E. coliDepositorInsertchmN-C47A
UseTagsHis6ExpressionBacterialMutationchanged Cys 47 to AlaPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3L-TwStrep
Plasmid#208649Purposefor E. coli expression of C-ter Twin Strep tagged human RPL3L (ENST00000268661), contain SNP rs753338989, rs746044959, rs752134949DepositorInserthuman RPL3L (ENST00000268661) (RPL3L Human)
UseTags6xHis and Twin StrepExpressionBacterialMutationcontain rs753338989, rs746044959, rs752134949, pr…PromoterT7Available sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-Flag-RBM24
Plasmid#208621Purposefor E. coli expression of Flag tagged human RBM24 (ENST00000379052)DepositorInserthuman RBM24 (ENST00000379052) coding region (RBM24 Human)
UseTags6xHis and FlagExpressionBacterialMutationPromoterT7Available sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-pro-IL-18_E192K_D193K
Plasmid#214322PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationE192K_D193KPromoterT7Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-pro-IL-18_I48N
Plasmid#214323PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationI48NPromoterT7Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Caspase-4_sheep_catalytic domain
Plasmid#214331PurposeProtein overexpression in bacteriaDepositorInsertCASP4
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_Caspase-11_humanized-mutant
Plasmid#214315PurposeProtein overexpression in bacteriaDepositorInsertCASP4 (Casp4 Mouse)
UseTagsHis-TEVExpressionBacterialMutationN152K_KLS288YKT_KASIHS348TPRAKAPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only