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Plasmid#174442Purposein vitro transcription template for dCas9-EGFPDepositorInsertCas9 Endonuclease Dead
UseIn vitro transcription templateTagsEGFPExpressionMutationPromoterT7 SP6Available sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P23
Plasmid#120034PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with HaloDepositorInsertHalo
UsePcr templateTagsHaloExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mas-KB1691-Nluc
Plasmid#204324PurposeGPCR/G protein BRET biosensorDepositorInsertKB1691-Nluc
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-hSyn-Galpha-q ONE-GO
Plasmid#204336PurposeGPCR/G protein BRET biosensorDepositorInserthSyn Galpha-q ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P34
Plasmid#120030PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xFLAGDepositorInsert3xFLAG
UsePcr templateTags3xFLAGExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
ArcLight-A242
Plasmid#36857DepositorInsertfluorescent protein voltage sensor
UseTagsExpressionMammalianMutationCi-VSP contains R217Q mutation; super ecliptic pH…Promotersemian CMVAvailable sinceAug. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P05
Plasmid#120016PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with eGFPDepositorInserteGFP
UsePcr templateTagseGFPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpCas9_sgRNA_expression_in_pBluescript
Plasmid#122089PurposeU6 driven SpCas9 sgRNA expression vector for cloning own guidesDepositorInsertSpCas9 tracr (NEWENTRY Synthetic, S. pyogenes)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
MmSphk1
Plasmid#118599PurposeExpresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in miceDepositorInsertMus musculus sphingosine kinase 1
UseTagsFLAGExpressionYeastMutationPromoterGAL1, 10Available sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
HsSPHK1
Plasmid#118091PurposeExpresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)DepositorInsertHomo sapiens Sphingosine Kinase 1
UseTagsFLAGExpressionYeastMutationPromoterGAL1, 10Available sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P28
Plasmid#120039PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xHADepositorInsert3xHA
UsePcr templateTags3xHAExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-mCh-empty
Plasmid#21907PurposeCre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.DepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2009AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA Donor_CAG
Plasmid#229780PurposeBxb1-GA donor plasmid for the cloning of payloads <20 kb with CAG promoter for expressing landing pad enrichment markerDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLVX-hSyn-Galpha-i3 ONE-GO
Plasmid#204334PurposeGPCR/G protein BRET biosensorDepositorInserthSyn Galpha-i3 ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCK01_PalkS_alkS
Plasmid#166502PurposeExpression of AlkS transcriptional regulator with L401G mutation. Sensor input plasmid for detection of n-butanol and branched-chain higher alcohols.DepositorInsertalkS
UseSynthetic BiologyTagsExpressionBacterialMutationL401GPromoterPalkSAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC0073 EiCsm6 His6-TwinStrep-SUMO-BsaI
Plasmid#115268PurposeExpresses EiCsm6 from a SUMO-tagged bacterial expression constructDepositorInsertEiCsm6
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV Puro-CCSP:GFP
Plasmid#68487PurposeRetrovirus expressing GFP driven by CCSP promoterDepositorInsertsPuromycin Resistance
GFP
UseRetroviralTagsExpressionMammalianMutationPromoterCCSP and LTRAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-cyto-KB1691-Nluc
Plasmid#204326PurposeGPCR/G protein BRET biosensorDepositorInsertcytosolic KB1691-Nluc
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_Z18-PDGFR_EF1a_eGFP
Plasmid#186304PurposePlasmid for inducible mammalian cell expression of the Z18 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction.DepositorInsertsZ18-PDGFR
eGFP
UseTagsHA, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
IL-8 toehold switch sensor
Plasmid#111908PurposeToehold switch sensor to detect IL-8 mRNA with GFP outputDepositorInsertIL8 toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only