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pAAV-CAG-dLight1.1

Plasmid

#111067

Purpose

To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has Service

AAV1 and AAV5

Insert

dLight1.1

Use

AAV

Tags

Flag tag

Expression

Mutation

Promoter

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-dLight1.1

Plasmid

#111066

Purpose

To generate Adeno-Associated viruses for expression of dLight1.1 under synapsin promoter

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has Service

AAV1

Insert

dLight1.1

Use

AAV

Tags

Flag tag

Expression

Mutation

Promoter

Available since

July 23, 2019

Availability

Academic Institutions and Nonprofits only
IMPT-10314:GABBR2

Plasmid

#157851

Purpose

HA-3xFLAG-VFT-TMD-CC in pFastBac1 for Sf9 expression

Depositor

Raymond Stevens, Vadim Cherezov

Article

Shaye et al Nature. 2020 Jun 17. pii: 10.1038/s41586

Insert

GABBR2 (GABBR2 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-dLight1.3b

Plasmid

#135762

Purpose

For expression of the genetically encoded dopamine sensor dLight1.3b

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has Service

AAV9

Insert

dLight1.3b

Use

AAV

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-dLight1.2

Plasmid

#111068

Purpose

To generate Adeno-Associated viruses for expression of dLight1.2 under synapsin promoter

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has Service

AAV1 and AAV5

Insert

dLight1.2

Use

AAV

Tags

Flag tag

Expression

Mutation

Promoter

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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p416-TEF-membrane localization signal-tagBFP-wrmScarlet11-TEF terminator

Plasmid

#158585

Purpose

Expresses membrane localized tagBFP with wrmScarlet11 C-terminal fusion in S. cerevisiae

Depositor

Cynthia Kenyon

Article

Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

Insert

membrane localization signal-tagBFP-wrmScarlet11

Use

Tags

tagBFP

Expression

Yeast

Mutation

GGGS linker

Promoter

TEF

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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IMPT-2070

Plasmid

#172092

Purpose

pFastBac1 vector with GP64 promoter replacing polH promoter expressing A2aAR+BRIL GPCR

Depositor

Raymond Stevens

Article

Liu et al Science. 2012 Jul 13;337(6091):232-6. do

Insert

Homo sapiens adenosine A2a receptor (ADORA2A) (ADORA2A Human)

Use

Tags

cytochrome b562RIL

Expression

Insect

Mutation

Promoter

gp64

Available since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-dLight1.2

Plasmid

#111069

Purpose

To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has Service

AAV1

Insert

dLight1.2

Use

AAV

Tags

Flag tag

Expression

Mutation

Promoter

Available since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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His-ZZ-TEV-BICDR1

Plasmid

#111859

Purpose

Full length BICDR1 for expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8x-His-ZZ.

Depositor

Andrew Carter

Article

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

BICDR1 (Bicdl1 Mouse)

Use

Tags

His-tag, TEV site, and ZZ

Expression

Insect

Mutation

Promoter

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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Sandgrouse+R111Q–insD

Plasmid

#191229

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al Mol Biol Evol. 2022 Dec 6:msac258. doi:

Inserts

ATP1A1
ATP1B1

Use

Tags

Expression

Insect

Mutation

removed aspartic acid at 121 and replaced arginin…

Promoter

PH and p10

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-dLight1.3b

Plasmid

#125560

Purpose

For expression of the genetically encoded dopamine sensor dLight1.3b

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has Service

AAV1, AAV5, and AAV9

Insert

dLight1.3b

Use

AAV

Tags

Flag tag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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His-ZZ-TEV-SNAPf-BICDR1

Plasmid

#111858

Purpose

N-terminal SNAPf tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.

Depositor

Andrew Carter

Article

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

BICDR1 (Bicdl1 Mouse)

Use

Tags

His-tag, SNAPf-tag, TEV site, and ZZ

Expression

Insect

Mutation

R250Q, Optimised for Sf9 expression

Promoter

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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RAT

Plasmid

#187416

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al bioRxiv 2021.11.29.470343

Inserts

ATP1A1 (Atp1a1 Rat)
ATP1B1 (Atp1b1 Rat)

Use

Tags

Expression

Insect

Mutation

Promoter

PH and p10

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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His-ZZ-TEV-BICDR1-GFP

Plasmid

#111657

Purpose

C-terminal GFP tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-GFP N-terminal tag, TEV site to cleave 8xHis-ZZ.

Depositor

Andrew Carter

Article

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

BICDR1-GFP (Bicdl1 Mouse)

Use

Tags

8xHis-tag, GFP, TEV site, and ZZ

Expression

Insect

Mutation

Optimised for Sf9 expression

Promoter

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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His-ZZ-TEV-BICDN(1-400)-GFP

Plasmid

#111862

Purpose

C-terminal GFP tag on BICDN (amino acids 1-400) for baculovirus expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8xHis-ZZ.

Depositor

Andrew Carter

Article

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

BICDN(1-400)-GFP (Bicd2 Mouse)

Use

Tags

8xHis tag, GFP, and ZZ tag

Expression

Insect

Mutation

Codon optimized for Sf9 expression

Promoter

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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Hook3(1-522)-SNAPf-Psc-StrepII

Plasmid

#111860

Purpose

C-terminal SNAPf tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal SNAPf-Psc-StrepII tag.

Depositor

Andrew Carter

Article

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

Hook3(1-552)-SNAPf (HOOK3 Human)

Use

Tags

Psc, SNAPf, and Strep-II

Expression

Insect

Mutation

Optimised for expression in Sf9 cells

Promoter

Available since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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Hook3(1-522)-GFP-Psc-StrepII

Plasmid

#111861

Purpose

C-terminal GFP tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal GFP-Psc-StrepII tag.

Depositor

Andrew Carter

Article

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

Hook3(1-552)-GFP (HOOK3 Human)

Use

Tags

GFP, Psc tag, and Strep tag

Expression

Insect

Mutation

Optimised for expression in SF9 cells

Promoter

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ASAP3-WPRE

Plasmid

#132331

Purpose

expresses ASAP3(ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vector

Depositor

Michael Lin

Article

Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

Insert

ASAP3

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSynapsin

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-FAB(PX-LD)

Plasmid

#214422

Purpose

Detection of peroxisome-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

PMP34-FKBP-V5-RspAN_IRES_CFAST-20nm-Halo-6xHp NC (SLC25A17 Human)

Use

Lentiviral

Tags

CFAST-Halo and FKBP-V5-RspAN

Expression

Mutation

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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RAT +D122H

Plasmid

#187426

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al bioRxiv 2021.11.29.470343

Inserts

ATP1A1 (Atp1a1 Rat)
ATP1B1 (Atp1b1 Rat)

Use

Tags

Expression

Insect

Mutation

changed aspartic acid at 129 for histidine

Promoter

PH and p10

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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