We narrowed to 3,756 results for: biorxiv
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Plasmid#170034PurposeEncodes LacI-controlled expression of surface-displayed GNLY and PvirK-mNeonGreen reporterDepositorArticleInsertsUseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only
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pGL0_16 [2μ ORI]
Plasmid#198929PurposeLevel 0 partDepositorInsert2μ ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tol2_B-act_dCas9-Vp64-T2A-Citrine
Plasmid#200248PurposeZebrafish transgenics; Plasmid encodes dCas9-Vp64 and could be stably integrated in the zebrafish genome using Tol2 transgenesisDepositorInsertdCas9-Vp64-T2A-Citrine
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
ITPKA_3xmTurquoise
Plasmid#112961PurposeIn vivo visualization of F-actin (can be used for colocalization studies)DepositorArticleInsertITPKA
UseTags3x mTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_YTHDF1-Halo
Plasmid#175301PurposeProduction of HaloTag fusion proteinDepositorInsertYTHDF1 (YTHDF1 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSCBE3-HF
Plasmid#133967PurposeExpresses HF-BE3 CRISPR base editor and gRNA scaffold in StreptomycesDepositorArticleInsertHF-BE3
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL1_39
Plasmid#199046PurposeLevel 1 plasmid, barcoded ORIDepositorInsertpSa ORI plus NGS barcode 16
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL1_36
Plasmid#199043PurposeLevel 1 plasmid, barcoded ORIDepositorInsertpBBR1 ORI plus NGS barcode 13
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_17 [AMA1 short ORI]
Plasmid#198930PurposeLevel 0 partDepositorInsertAMA1 short ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_21 [UARS1 ORI]
Plasmid#198934PurposeLevel 0 partDepositorInsertUARS1 ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
dSp-VP64-RTA
Plasmid#99675PurposeExpresses dSp Cas9 fused at C term. To VP64 and RTA activation domainsDepositorArticleInsertdCas9
UseTagsVP64-RTAExpressionMammalianMutationdead Cas9PromoterAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL0_11 [pWKS1 ORI]
Plasmid#198925PurposeLevel 0 partDepositorInsertpWKS1 ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTH848-gst-disec
Plasmid#89589PurposePlasmid for bacterial expression of His-tagged GST encoded by non-preferred codonsDepositorArticleInsertglutathione-S transferase codon-dis-optimised for bacterial expression
UseTags8xhistidineExpressionBacterialMutationPromoterT7Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
dnCEBP: pLenti-TRE-A-C/EBP-PGK-rtTAA-2A-Puro
Plasmid#177201Purposeencoding a dominant negative form of CEBP under inducible promoterDepositorArticleInsertdnCEBP (A-C/EBP)
UseLentiviralTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmCherry2-CD86-mEYFP(Q69K)
Plasmid#190749PurposeExpression of CD86 double tagged with mCherry2 (N-term) and mEYFP(Q69K) (C-term) in mammalian cells.DepositorInsertCD86 (CD86 Human)
UseTagsmCherry2 and mEYFP(Q69K)ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TRE3G-NODAL-matMYC
Plasmid#115639PurposeFor targeted integration and inducible expression of human NODAL (with an N-terminal MYC tag on the mature peptide) using doxycyclineDepositorInsertNODAL (NODAL Human)
UseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUAS-kkv-mos::NG
Plasmid#138958PurposeUsed to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila where the active site is converted to the mosquito sequence. Expression by using Gal4DepositorArticleInsertkkv (NEWENTRY Fly)
UseUas transgene vectorTagsNeon Green fused to C terminus of KkvExpressionInsectMutation8 bp that are in the region that encodes the cata…PromoterUASAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
HisSumoOphP
Plasmid#189672PurposeHisSUMO tagged OphP to express OphP in pichia pastorias.DepositorInsertHisSumoOphP
UseTagsHisSumo taggedExpressionYeastMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKB234.2
Plasmid#170023PurposeEncodes LacI-controlled expression of surface-displayed hBD3 fragment lacking alpha-helix sequence and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
hBD3 Δ alpha-helix
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceDec. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TagBFP-P2A-Pre-SPHERE-sfVenus-iGluSnFR
Plasmid#187097PurposeGlutamate sensor for localization to membrane contatctDepositorArticleInsertPre-SPHERE-sfVenus-iGluSnFR
UseTagsTagBFP-P2AExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only