Addgene

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pJW4.17

Plasmid

#62872

Purpose

Bacterial expression of MBP-MBK-2 K196R

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

MBP-MBK-2 K196R

Use

Tags

MBP

Expression

Bacterial

Mutation

K196R

Promoter

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only
pNH002

Plasmid

#42145

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

N-TAP TAG::[(G4S)3]::mTFP1

Use

Tags

TAP TAG

Expression

Worm

Mutation

Promoter

Available since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pNH026

Plasmid

#42148

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1::linker::TAP TAG

Use

Tags

TAP-Tag

Expression

Mutation

Promoter

Available since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pNH001

Plasmid

#42144

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

TAP-tag

Use

Tags

TAP-tag

Expression

Worm

Mutation

Promoter

Available since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pCFJ1000

Plasmid

#44475

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, PuroR, peel-1, [4-3] Gateway

Use

Gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pAS3-rab-3P::AI::lite-1G::SL2::his-24::tagBFP

Plasmid

#232610

Purpose

Pan-neuronal expression of 'LITE-1' using a genomic fragment and fluorophore 'tagBFP', regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

LITE-1 (lite-1 Nematode)

Use

Tags

Expression

Worm

Mutation

N/A

Promoter

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAS3-rab-3P::AI::gur-3G::SL2::his-24::tagBFP

Plasmid

#232608

Purpose

Pan-neuronal expression of 'GUR-3' using a genomic fragment and fluorophore 'tagBFP', regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

GUR-3 (gur-3 Nematode)

Use

Tags

Expression

Worm

Mutation

N/A

Promoter

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAS3-rab-3P::AI::prdx-2G::SL2::his-24::tagRFP

Plasmid

#232609

Purpose

Pan-neuronal expression of 'PRDX-2' using a genomic fragment and fluorophore 'tagRFP', regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

PRDX-2 (prdx-2 Nematode)

Use

Tags

Expression

Worm

Mutation

N/A

Promoter

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pHW1221

Plasmid

#229873

Purpose

gene trap RMCE plasmid with QF-act-4 3' UTR::SEC for RMCE cloned in the destination vector pHW1133 to swap driver via RMCE

Depositor

Han Wang

Article

Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

Insert

QF-act-4 3' UTR::SEC

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pHW1143

Plasmid

#229866

Purpose

Entry vector with FRT-SA-ArtificialExon-3xStopCodons-SL2-AAAA in slot 4 (for SapTrap assembly)

Depositor

Han Wang

Article

Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

Insert

FRT-SA-ArtificialExon-3xStopCodons-SL2-AAAA

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pMS18

Plasmid

#215675

Purpose

Cas9 + guide plasmid for inserting ChrI split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

eef-1A.1p::Cas9 + U6p::GAAATCGCCGACTTGCGAGG

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMS62

Plasmid

#215676

Purpose

Cas9 + guide plasmid for inserting ChrIII split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

eef-1A.1p::Cas9 + U6p::GTCGTTCTTCCGTTCTCGGG

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pZCS41 (U6p::GCGAAGTGACGGTAGACCGT)

Plasmid

#193050

Purpose

Encodes guide RNA expression targeting PX740 landing pad

Depositor

Patrick Phillips

Article

Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

Insert

Guide RNA

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pHIT318

Plasmid

#204508

Purpose

rpl-21 promoter-driven C04F12.1 construct tagged with 2xHA for MosSCI integration at locus ttTi5605 on Chr II in nematode

Depositor

Yuji Kohara

Article

Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

Insert

rpl-21p::2xHA::C04F12.1 (C. elegans)

Use

Tags

2xHA

Expression

Worm

Mutation

Promoter

rpl-21 (C. elegans)

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pHIT246

Plasmid

#204507

Purpose

csr-1 construct tagged with 2xFLAG for MosSCI integration at locus ttTi5605 on Chr II in nematode

Depositor

Yuji Kohara

Article

Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

Insert

2xFLAG::csr-1 (C. elegans) (csr-1 Nematode)

Use

Tags

2xFLAG

Expression

Worm

Mutation

Promoter

csr-1 (C. elegans)

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2-W390A_P

Plasmid

#147236

Purpose

Insect Expression of CeAIN2-W390A

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

CeAIN2-W390A

Use

Tags

Expression

Insect

Mutation

Deletion of two AA SD 378-379 compared to the seq…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-CeAIN1_1-504_P

Plasmid

#147238

Purpose

Insect Expression of CeAIN1_1-504

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

CeAIN1_1-504 (ain-1 Nematode)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-CeNot1_P

Plasmid

#147239

Purpose

Insect Expression of CeNot1

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

CeNot1

Use

Tags

Expression

Insect

Mutation

one non silent mutation A610T compared to the seq…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-CePAN3_1-244_P

Plasmid

#147243

Purpose

Insect Expression of CePAN3_1-244

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

CePAN3_1-244

Use

Tags

Expression

Insect

Mutation

Insertion of two AA (FQ) at position 172 compared…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN1_1-504_P

Plasmid

#147227

Purpose

Insect Expression of CeAIN1_1-504

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al Nucleic Acids Res. 2012 Jul;40(12):5651-

Insert

CeAIN1_1-504 (ain-1 Nematode)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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